Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 387 results for: nans

Showing: 361 - 387 of 387 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...droplet digital PCR (ddPCR) to titer purified recombinant Adeno-associated viral vectors (AAV). This protocol...

  3. Finding Your Science Policy Path

    Type
    Blog Post
    ...writing for the Journal of Science Policy and Governance.  Get involved! The best way to learn about science...

  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ...cells. Pass the cells twice a week during the maintenance phase and do not allow cells to reach 100% confluence...

  8. Typing CRISPR Systems

    Type
    Blog Post
    ...https://doi.org/10.1038/nature24049 Altae-Tran, H., Kannan, S., Suberski, A. J., Mears, K. S., Demircioglu...

  17. Adenovirus Guide

    Type
    Guide
    ...in a higher background of non-recombinant adenoviral plasmids. Recombinant adenoviral plasmids are then...vectors for recombinant adenoviral vector production that do not contain GFP. Recombinant adenoviral vector...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...information about AAVs, read our AAV guide . Recombinant Adenoviral Vectors Wild-type adenoviruses have...have been modified by researchers to create recombinant adenoviral (rAdV) vectors in order to deliver genetic...recombination have not occurred. Once verified, the recombinant plasmid is linearized with PacI to create a linear.... Common Uses of Adenoviral Vectors Although recombinant adeno-associated viral (rAAV) vectors are often...

  18. Antibody Guide

    Type
    Guide
    ...Antibodies produced this way are called recombinant antibodies. Recombinant antibodies are created by cloning... time and labor intensive. Recombinant expression validation A recombinant expression strategy is used...cultured immune cells, or through the use of recombinant plasmids. Animals - Antibodies produced in animals... of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from plasmids...abundant isotype in blood, also found in tissues; predominant isotype in secondary immune response Monomer ...Single-domain antibodies (sdAbs), also called nanobodies, are ~12-15 kD single heavy domain fragments ...Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody (IgG, far left) compared...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...Guide . Recombinant AAV Vectors Wild-type AAVs have been modified by researchers to create recombinant adeno-associated...Parvoviridae family. AAVs were discovered as a contaminant of adenovirus preparations, which is how they...in order to deliver genetic cargo into cells. Recombinant AAV constructs are designed by replacing the ...Created with BioRender.com. Genome Integration Recombinant AAVs are generally considered to not integrate...cystic fibrosis and cancer. Neuronal Tracing Recombinant AAV vectors have been widely used by neuroscientists...P. J., Mandel, R. J., & Muzyczka, N. (2004). Recombinant AAV viral vectors pseudotyped with viral capsids...sites in AAV virions during AAV wild-type and recombinant AAV vector production . Journal of Virology, ...

  20. Molecular Biology Reference

    Type
    Guide
    ...encoded for by multiple codons. Plasmids and Recombinant DNA Technology Techniques in chemistry enable...This obstacle has been overcome by the field of recombinant DNA technology, which enables the preparation... reference to any extrachromosomal heritable determinant. Plasmids are fragments of double-stranded DNA...plasmid. These tools are essential to the field of recombinant DNA, in which many identical DNA fragments can...a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study DNA ...fragments of interest, such as genes. Creation of recombinant DNA Molecular Cloning Plasmids that are used ...used most commonly in the field of recombinant DNA technology have been optimized for their use of studying...

  21. Optogenetics Guide

    Type
    Guide
    ...196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I,...PMID 20621963 Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M...Saunders A, Johnson CA, Sabatini BL 2012. Novel recombinant adeno-associated viruses for Cre activated and...

  22. Cloning

    Type
    Guide
    ...Stitching Molecular cloning or the creation of recombinant DNA is an essential process used in scientific... most popular cloning methods used to create recombinant DNA. Restriction Enzyme Cloning Restriction enzyme...

  23. CRISPR Guide

    Type
    Guide
    ...Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli, C., & Concordet, J. P. (2018). CtIP fusion ...prime editing systems by manipulating cellular determinants of editing outcomes. Cell . 184 (22), 5635-5652...

  24. Chemogenetics Guide

    Type
    Guide
    ...Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019...

  25. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...Smith, K., Price, J., Srivastava, S., Hussain, R., Banani, M. A., Day, W., Stevenson, E., Madigan, M., Chen...

  26. Plan Your Experiment

    Type
    Guide
    ...expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host genome. Scientists...

  27. Lentiviral Vector Guide

    Type
    Guide
    ...integration site preferences. A major cellular determinant is LEDGF (also known as PSIP1 or p75 ), a lentiviral...
Showing: 361 - 387 of 387 results