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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 267 results for: ttl

Showing: 241 - 260 of 267 results

  1. Cloning

    Type
    Guide
    ...entry clone). The entry clone now has recombined attL sites flanking your DNA fragment of interest. Now...cloned into a donor plasmid, it can be rapidly shuttled into any compatible Gateway® Destination vector...

  2. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Loosely close the cap on the bottle (do NOT close all the way or the bottle may explode!) and then loosely...LB, weigh out the following into a 500 mL glass bottle: 4 g NaCl 4 g Tryptone 2 g Yeast Extract and dH...loosely cover the entire top of the bottle with aluminum foil. Autoclave and allow to cool to room temperature...temperature. Now screw on the top of the bottle and store the LB at room temperature. When ready to grow ...

  3. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...usually use one or the other, but there is very little difference between the two. Note: Make sure to ...increases the density of your DNA sample causing it settle to the bottom of the gel well, instead of diffusing...later use, use long-wavelength UV and expose for as little time as possible to minimize damage to the DNA....

  4. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...ideal conditions with very clean DNA, so using a little more enzyme is advisable. Reactions are often performed...enzyme than you will need, but that's okay because a little more enzyme is usually better. Mix gently by pipetting...

  5. Pipetting Protocol

    Type
    Protocol
    ...Containers to hold measured liquid (ex: microfuge tube, bottle, etc.) Labels for containers Reagents Liquid for...measured liquid (ex: another microcentrifuge tube, a bottle etc.). If there is already other liquid in the ...

  6. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...PEI-MAX powder to 900 mL deionized water in a 1 L bottle and stir on a magnetic stir plate. Stir until all...into a clean microcentrifuge tube. Pro-Tip Cells settle quickly and need to be resuspended before sampling...

  7. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...X PBS, 1X pH 7.4, VWR 45000-446 250 mL sterile bottles, Corning 430281 Deionized water IgG isotype standard...LC) proteins, respectively. There should be very little background staining. Pro-Tip A large shift in the...

  9. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ... Pipettes, 10 mL, VWR 89130-898 1 L polystyrene bottle, Corning 430518 PBS, 1X pH 7.4, VWR 45000-446 TMB... mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several times to mix. Carefully remove...

  10. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...especially in the corner of the well as cells tend to settle there. Once the cells have expanded but before ...

  11. Guide to Using Pooled Libraries

    Type
    Guide
    ...winners’. Negative Screen Negative screens are a little trickier than positive screens. In a negative screen...

  12. Plan Your Experiment

    Type
    Guide
    ...DNA to serve as template for the repair. Once you settle on your gRNA, you can design a donor DNA to have...

  14. Antibody Guide

    Type
    Guide
    ...produce large amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can...

  15. DNA Quantification

    Type
    Protocol
    ...accurately read DNA concentration and purity in as little as 1μl. Regardless of whether you have a NanoDrop...

  18. Fluorescence Titering Assay

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...

  19. General Transfection

    Type
    Protocol
    ...stable alternative such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...

  20. Lentivirus Production

    Type
    Protocol
    ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...
Showing: 241 - 260 of 267 results