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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 400 results for: pho;

Showing: 391 - 400 of 400 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...envelope, Phoenix-ECO (for infection of mouse and rat cells), or an amphotropic envelope, Phoenix-AMPHO (for...of the viral packaging cell line. For example, Phoenix™ is a popular second-generation gamma-retroviral... produced in just a few days. Another variant, Phoenix-gp, contains only gag-pol and enables additional...

  2. Chemogenetics Guide

    Type
    Guide
    ...activate neuronal firing through stimulating phospholipase C, which releases intracellular calcium stores...molecule driven by FRET-modified bioluminescence. Neurophotonics. 11:021005. PMID: 38450294 (Link opens in a...luminopsins with improved coupling efficiencies. Neurophotonics. 11:024208. PMID: 38559366 (Link opens in a...

  3. CRISPR Guide

    Type
    Guide
    ...SpCas9-HF1 - disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading...fusing the anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled...: Epigenetics Visualizing Genomic Loci Using Fluorophores CRISPR imaging has numerous advantages, including...11: Overview of visualizing genomic loci with fluorophores using CRISPR Browse Plasmids: Visualize Purify...Yoshimi, K., Okuzaki, Y., Gee, P., Kunihiro, Y., Sonpho, E., Xu, H., Sasakawa, N., Naito, Y., Nakada, S...

  4. Molecular Biology Reference

    Type
    Guide
    ...of restriction enzymes, DNA ligase, and gel electrophoresis allowed for the ability to move specific fragments...Delta(lacZYA-argF) U169 recA1 endA1 hsdR17(rk-, mk+) phoA supE44 thi-1 gyrA96 relA1 tonA JM109 Addgene; Promega...4 standard bases are tagged with a different fluorophore so they can be distinguished from one another...

  5. Promoters

    Type
    Guide
    ...promoter PGK Constitutive Mammalian promoter from phospholycerate kinase gene TRE Inducible Tetracycline response...

  6. Plan Your Experiment

    Type
    Guide
    ...gRNA Presence of a selectable marker (drug or fluorophore) Delivery method Expression System Components...identify DNA fragments. PCR amplification and gel electrophoresis (for HDR or NHEJ): For large deletions or insertions...

  7. Educational Resources

    Type
    Guide
    ...plasmid cloning, and virus, including: Gel Electrophoresis Bacterial Transformation Plasmid Cloning Creating...

  8. Addgene's eBook Collection

    Type
    Guide
    ...localization; an introduction to non-protein fluorophores; and much more! Close About Overlay Select Viral...

  9. Lentiviral Vector Guide

    Type
    Guide
    ... are other envelope gene options, including: Lymphocytic Choriomeningitis Virus (LCMV) – less toxic and...
Showing: 391 - 400 of 400 results