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We narrowed to 327 results for: phy

Showing: 301 - 320 of 327 results
  1. Typing CRISPR Systems

    Type
    Blog Post
    ...relies on a combination of sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding...
  2. Deep Dive: qPCR

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    Blog Post
    ...can up-regulate the expression of GAPDH. Biochem Biophys Res Commun. 2001 Mar 9;281(4):951-6. doi: 10.1006...
  3. Using AAV for Neuronal Tracing

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    Blog Post
    ... with other neuroscience methods such as electrophysiological recordings. The origins of these methods...
  4. 15 Hot Plasmids from 2017

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    Blog Post
    ...popular Optogenetics tools use light to alter cell physiology and molecular processes via genetically encoded...
  5. Optogenetics Guide

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    Guide
    ...dimerization of Phytochrome Interacting Factor 3 or 6 (PIF3 or PIF6) with PhyB 660 PhyA/FYH1 and PhyA/FHL Light-controlled...is also available 450 Phytochromes Name Description Peak excitation λ (nm) PhyB/PIF Light-controlled, ...Light-controlled reversible dimerization of PhyA with FHY1 or FHL; PhyA and FHY1 also known as REDMAP 660 BphP1...future developments. Exp Physiol, 96 (1), 19–25. https://doi.org/10.1113/expphysiol.2009.051961 PMID: 20621963...neural activity: The biophysics of microbial rhodopsins in neuroscience. Q Rev Biophys, 57 , e1. https://...Optogenetics has been broadly applied to study the physiology of the brain and nervous system to better map...ChR1 because ChR2 has higher conductance at physiological pH and traffics well to the membrane. The optogenetic...
  6. Chemogenetics Guide

    Type
    Guide
    ...and Neuronal Biology. Physiol Rev, 98 (1), 391–418. https://doi.org/10.1152/physrev.00009.2017 PMID: 29351511...neurons expressing the LMO, depending on its biophysical properties. While LMOs provide chemogenetic access...Luminopsins integrate opto- and chemogenetics by using physical and biological light sources for opsin activation.../pnas.1510899113 PMID: 26733686 Björefeldt, A., Murphy, J., Crespo, E. L., Lambert, G. G., Prakash, M....
  7. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...disease, spinal muscular atrophy, hemophilia A and B, and Duchenne muscular dystrophy. Many clinical trials...be included between the ITRs is limited by the physical space available inside of the small AAV capsid...smaller Cas9 orthologs, such as SaCas9 from Staphylococcus aureus . The Feng Zhang lab has developed plasmids...
  8. CRISPR Guide

    Type
    Guide
    ... Cas9 orthologs are available. SaCas9 (from Staphylococcus aureus ) has a coding sequence ~1 kb shorter...NGNG xCas9 3' NG, GAA, or GAT SpCas9-NG 3' NG Staphylococcus aureus (SA); SaCas9 3' NNGRRT or NNGRR(N) Acidaminococcus...Zhang, F. (2015). In vivo genome editing using Staphylococcus aureus Cas9. Nature , 520 (7546), 186–191. ...immunoprecipitation (enChIP) using CRISPR. Biochemical and Biophysical Research Communications . 439 (1), 132–136. https...
Showing: 301 - 320 of 327 results