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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 372 results for: Fus

Showing: 341 - 360 of 372 results

  1. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... S. pyogenes Sternberg pJW1311 61254 Worm none, fusion PCR none S. pyogenes Ward pCFD1-dU6:1gRNA 49408... S. pyogenes Yang pUC gRNA Shuttle 47024 Plant inFusion none S. pyogenes Parrott pCBC-DT1T1 50590 Plant...

  2. Antibody Guide

    Type
    Collection
    ...cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized B cells...fluorophores with minimal signaling overlap to avoid confusing data. Check that your machine has appropriate ...

  3. Optogenetics Guide

    Type
    Guide
    ...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation... an optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). ...point mutations, codon optimization, and chimeric fusion of domains from two different ChRs. These ChR variants... given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated...-part transcription factors - Cry2 and CIB1 are fused to a transcriptional activation domain and DNA binding..., both LOV and Dronpa can control activity of a fused protein through allosteric interactions. Researchers...cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control...

  4. Modular Cloning Guide

    Type
    Guide
    ...possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs. FusX TALEN Assembly System...and ligated in a single step resulting in a 4-bp fusion site in between. MoClo can be used for a variety...coding sequences, terminators, etc.) flanked by fusion sites into individual Level 0 plasmids, or choose...Expression Paola Branduardi Easy-Modular Integrative fuSion-ready Expression toolkit for improved heterologous... a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use in human or mouse systems. Fragmid Genome... Genome Engineering, TALEN Takashi Yamamoto Nine pFUS array vectors and destination vectors designed for...TALEN Stephen Ekker 336 plasmids of pre-assembled pFusX clones allowing for Golden Gate assembly of 15-16...

  5. Tags and Other Markers

    Type
    Collection
    ...recombinant antibodies targeting epitope tags and common fusion proteins....

  6. Cloning

    Type
    Guide
    ...overhangs, or which are both blunt-ended, can then be fused together during a ligation reaction with T4 DNA ...different molecular biology techniques (such as fusing it with different tags, putting it under a variety...ready TOPO® vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase acts as a ligase...molecules using the 4 nucleotides and lastly DNA ligase fuses DNA strands together. In Gibson assembly DNA fragments.... Similar to Gibson, this method can efficiently fuse two (or more) fragments of dsDNA that have 30 or... of DNA that share no end homology can still be fused together in a seamless manner. To accomplish this... (or more) fragments of DNA that you would like fused along with custom ordered DNA oligos of 60-80 base...

  7. Zinc Finger Consortium Reagents

    Type
    Collection
    ...vectors for expressing zinc finger FokI nuclease fusions Related Genome Engineering Technologies TALEN Engineering...

  8. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...targeted epigenetic modifications, researchers have fused catalytically dead dCas9 to epigenetic modifiers...

  10. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Cas13a/C2c2 and Cas13b, target RNA rather than DNA. Fusing the catalytic domain of ADAR2(E488Q) adenosine ...

  12. Chemogenetics Plasmids

    Type
    Collection
    ... are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing...

  13. Optogenetics Plasmid Collection

    Type
    Collection
    ...light-oxygen-voltage-sensing (LOV) proteins. These fusions can then be used as an optical switch to control...

  16. Plan Your Experiment

    Type
    Guide
    ...or Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their...copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair...editing guide RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a...permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB...permanently modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64...transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need to be within...

  17. CRISPR Plasmids - C. elegans

    Type
    Collection
    ...PI Publication Activate Catalytically dead dCas9 fused to a transcriptional activator peptide can increase...

  18. AAV Molecular Tools

    Type
    Collection
    ...Cre-dependent expression of diphtheria toxin receptor fused to GFP for studying cell ablation. 2 Jessell , Azim...

  19. Antibody Plasmid Collection

    Type
    Collection
    ...the GCN4 peptide, from the popular SunTag system, fused to sfGFP for imaging. Learn more about antibodies...

  20. Distribution to Industry

    Type
    Collection
    ...Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-COV-2, ACE2, and CRISPR...
Showing: 341 - 360 of 372 results