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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 364 results for: gne

Showing: 341 - 360 of 364 results

  1. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...codons) with kanamycin cassettes and were carefully designed to allow for easy removal of the resistance cassette...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Rohatgi 133439 RG-001 EIF4G1 T7 Parkinson's Gerhard Wagner 133744 MSCV-human CSFI-R-L301S-IRES-GFP CSF1R MSCV...Rosalind Segal 167294 pLKO.1 puro_shTBK1_3185 TBK1 ALS Agnel Sfeir 169375 pET3d-SF-DCTN1-TS DCTN1 SplitFIAsH,...

  4. Water Bath Protocol

    Type
    Protocol
    ... of bacteria or fungi. There are disinfectants designed to be used in water baths with instructions on...

  6. Fluorescence Titering Assay

    Type
    Protocol
    ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  8. Colony Formation Titering Assay

    Type
    Protocol
    ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  9. Pipetting Protocol

    Type
    Protocol
    ...of the pipette. Select the pipette tip that is designed to fit your pipette. To load a tip onto a pipette...

  13. Western Blot

    Type
    Protocol
    ...Top Stack such that the electrical contacts are aligned with the corresponding electrical contacts on the...

  15. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Corning 25-015-CI) 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  16. Chemogenetics Guide

    Type
    Guide
    ...less than ideal. DREADDs Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), like RASSLs...Relationship Studies for Designer Receptors Exclusively Activated by Designer Drugs. ACS Chem Neurosci...1998). Controlling signaling with a specifically designed Gi-coupled receptor. Proc Natl Acad Sci USA. 95...functional characterization of a novel, arrestin-biased designer G protein-coupled receptor. Mol Pharmacol. 82:...

  17. Antibody Guide

    Type
    Guide
    ...agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic beads), collect...are two primary methods used, using agarose or magnetic beads, but both rely on the same general strategy...

  18. Molecular Biology Reference

    Type
    Guide
    ...Drives transcription of the insert. The promoter is designed to recruit transcriptional machinery from a particular...commonly used for plasmid selection Many plasmids are designed to include an antibiotic resistance gene, which...reaction. All the sequenced templates are then aligned to each other to assemble the entire sequence or...

  19. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...cargo into cells. Recombinant AAV constructs are designed by replacing the Rep and Cap regions between the...new window) Kwon, I., & Schaffer, D. V. (2008). Designer gene delivery vectors: molecular engineering and...., Schaffer, D. V., & Karpova, A. Y. (2016). A designer AAV variant permits efficient retrograde access...

  20. Guide to Using Pooled Libraries

    Type
    Guide
    ...effects of expressing multiple genes at once. A well-designed screen can help you begin to understand what genes...tube. Pooled libraries can be small, if they are designed to cover only a subset of genes, or very large...
Showing: 341 - 360 of 364 results