We narrowed to 371 results for: FUS

...The first deposited PITCh plasmids were tested by fusing EGFP-2A-PuroR cassette to a nucleolar protein, ...

...genetically-encoded nuclear Cry2 fusion can thus direct a CIB1-protein fusion to the nucleus upon light activiation... An optogenetics procedure. A channelrhodopsin, fused to mCherry, is expressed in neurons (red dots). ...point mutations, codon optimization, and chimeric fusion of domains from two different ChRs. These ChR variants... given protein, such as the Cre recombinase, is fused to Cry2 and the other half to CIB1, light-stimulated... transcription factors, where Cry2 and CIB1 are fused to a transcriptional activation domain and DNA binding..., both LOV and Dronpa can control activity of a fused protein through allosteric interactions. Researchers...cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1 to control...

... of interest. Inducible: The recombinase may be fused to a ligand-binding domain such as the human estrogen...

...possible combinations of pFUS_B4 clones for Golden Gate assembly of 15-RVD TALENs. FusX TALEN Assembly System...and ligated in a single step resulting in a 4-bp fusion site in between. MoClo can be used for a variety...coding sequences, terminators, etc.) flanked by fusion sites into individual Level 0 plasmids, or choose...Expression Paola Branduardi Easy-Modular Integrative fuSion-ready Expression toolkit for improved heterologous... a Cas9 nuclease, nickase, dCas9, or dCas9-FokI fusion for use in human or mouse systems. Fragmid Genome... Genome Engineering, TALEN Takashi Yamamoto Nine pFUS array vectors and destination vectors designed for...TALEN Stephen Ekker 336 plasmids of pre-assembled pFusX clones allowing for Golden Gate assembly of 15-16...

...or Cas9 nickase fusions with different base editing proteins) Many base editors fused to Cas9 exert their...copying from a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair...editing guide RNA (pegRNA) Prime editor (Cas9 nickase fused to reverse transcriptase (RT)) Can edit within a...permanently modifying the genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB...permanently modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64...transcription start site. CRISPRa requires dCas9 to be fused to transcriptional activators that need to be within...

..., and Sox2 as VP-16 transcriptional activating fusions in single lentiviral vectors for human iPSC generation...

...overhangs, or which are both blunt-ended, can then be fused during a ligation reaction with T4 DNA ligase. Restriction...endogenous DNA repair pathways will produce the fused product. One major advantage is that much larger...of DNA that share no homology ends can still be fused in a seamless manner. To accomplish this, you can...transform into yeast the fragments of DNA to be fused along with custom synthesized DNA oligos that span...

...the transfer plasmid contains a chimeric 5’ LTR fused to a heterologous promoter (often CMV or RSV), eliminating...Hybrid promoter; 5' LTR is partially deleted and fused to a heterologous enhancer/promoter such as CMV ...DNA integration and its effect on clinical pre-infusion CAR T-cell products. Journal of Translational ...

...), or luminescent opsins, are opto-chemogenetic fusion proteins of a light-emitting luciferase and a light-sensing...modules, such as luciferase-fluorescent protein fusions, to more-sensitive opsins has led to improved LMOs...

...cultures using hybridomas, cell cultures created by fusing B cells with myeloma cells (immortalized B cells...fluorophores with minimal signaling overlap to avoid confusing data. Check that your machine has appropriate ...

...widely throughout the organism to target large or diffused biological structures. Specifically, researchers...