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... and more. Luciferase Reporters Luciferase (Link opens in a new window) reporter assays use light produced...Find luciferase reporter vectors for sensitive gene expression analysis and fusion vectors containing...purpose-driven nonprofit, Addgene offers expert support, educational resources, and efficient material ... workflows. The collection contains luciferase reporter vectors for sensitive gene expression analysis...collection. For example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase"...includes specialized Promega technologies that support a range of applications. The technology includes... a new window) is a structural complementation reporter system composed of a Large BiT (LgBiT; 18 kDa)...

...CMV ALS Promega Corporation 237006 HaloTag-VCP Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET...research related to Alzheimer's Disease, ALS, Frontotemporal Dementia, Huntington's Disease, Parkinson's... (AD), Amyotrophic Lateral Sclerosis (ALS), Frontotemporal dementia (FTD), Huntington's Disease (HD), ... this page. Disease Alzheimer's Disease ALS Frontotemporal dementia Huntington's Disease Parkinson's Disease...'s Alexander Stark 71083 pFOSGE_CUS1A07 GRN Frontotemporal dementia (FTD) Alexander Stark 71433 pmGFP10C...Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI CACNA1A GFP U6, Cbh Spinocerebellar...)_Paragranulin+linker 1 GRN Flag, Strep CMV Frontotemporal dementia (FTD) Thomas Kukar 176917 pcDNA3.1...

...Envelope and Packaging Plasmids Reporter Plasmids A few examples of reporter plasmids that can be used for...-CoV-2 spike plasmids, packaging plasmids, and reporter plasmids for viral pseudotyping with SARS-CoV-...Plasmid Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials...44965 NL4-3 mCherry Luciferase Lentiviral dual reporter vector expressing mCherry and firefly luciferase...Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral reporter vector expressing firefly luciferase with neomycin... pHAGE-CMV-Luc2-IRES-ZsGreen-W Lentiviral dual reporter vector expressing firefly luciferase and ZsGreen...vector expressing GFP and luciferase for use as a reporter in pseudovirus production and infection. Dapeng...

...gRNA and HDR donor design. The current version supports SpCas9, SpCas9-VQR, and enAsCas12a Cas enzymes...Lab . Read more about it on the Addgene blog . CRISPOR (Link opens in a new window) This tool helps design...(2016) (Link opens in a new window) . Also try CRISPOR batch (Link opens in a new window) for lentiviral...input sequence and checks for off-target binding. Supports a range of Cas enzymes (Cas9 and Cas12) and organisms...regulation (repression and activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( ... or a sequence text file you upload. Currently supports a wide selection of organisms. From the Database...sequence and checks for off-target binding. Currently supports a wide range of organims. Developed by the Michael...

...luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase reporter plasmid...expression (Gateway) pYIC - Bicistronic fluorescent reporter gene with cap-dependent 3Myc-EYFP-HA-His6 and ...for viral vector delivery, genome modification, reporter assays, shRNA expression, transgenics, and more... the expression cassette of interest is incorporated into the host DNA. For mammalian cells, you ...page for more information Genome Modifications, Reporter Assays, mRNA Regulation, and More Find empty backbones...backbones for other uses such as genome modification, reporter assays, shRNA expression, transgenics, and more...TALEN array for genomic engineering Luciferase Reporter pGL3 Basic Vector - Firefly luciferase vector...

... has developed several tools useful for the incorporation of phosphoserine into your gene of interest.... where it recognizes the UAG amber codon and incorporates phosphoserine into the growing polypeptide chain...University changed the way researchers can explore important questions surrounding serine phosphorylation by... of the popular SepOTSλ (Plasmid #68292) . An important technical note: pSerOTS-C1* (V70) is a ROP minus...able to demonstrate unambiguous site-specific incorporation of phosphoserine in >36,000 phosphosites by ...expression vector encoding a split mCherry fluorescent reporter, enabling the detection of phosphorylation-dependent...phosphosite library is fused to the N-terminal portion of split mCherry, while a phosphobinding domain...

...testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference : Target...Predicted On-Target and Off-Target Activity Another important factor to keep in mind when designing your gRNA...In addition to off-target activity, it is also important to consider factors that maximize cleavage of ...generally perform by chemical transfection or electroporation. Expression can be transient, or you can generate... to target cells through microinjection or electroporation. The target cells translate the mRNA encoding...delivery the RNP to cells using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA... breaks will be repaired by NHEJ instead of incorporating your desired edit through HDR. Base and prime...

...Return to top Reporter Plasmids The table below highlights a selection of popular reporter plasmids that...plant-optimized plasmids for cloning, gene expression, reporter assays, genome editing, gene silencing, and more...Cloning & SynBio Genome Editing Gene Silencing Reporters Addgene’s repository contains many plasmid tools...from genome engineering and gene expression to reporter systems. Addgene’s collection includes plasmids...The following plasmid kits contain a variety of reporter plasmids for the study of protein–protein interactions... Golden Gate (MoClo) Benjamin Field Additional Reporter Plasmid Resources Visit our Fluorescent Protein...

...genomic instability and cell death. It is important to incorporate appropriate controls such as Cre-only ...Return to top Recombinase-Dependent Reporter Plasmids Incorporating a fluorescent protein into your expression...Plasmids Recombinase-Dependent Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase...recombinases , as well as useful empty backbones or reporters . Experimental Considerations Recombinase Recognition...visualize which cells have undergone recombination. Reporter plasmids can also be useful for lineage tracing...

...disorders. The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...and antibodies created with support from the BRAIN Initiative below. Support has been identified by depositors...The BRAIN Initiative supports the development of biomolecular tools for neuroscience research. Browse...plasmid encoding for Archon1 fluorescent voltage reporter, Cre-dependent expression Edward Boyden 108912...IRES-eGtACR1-ST Anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal...128485-AAV9 pAAV-CAG-iSeroSnFR-Nlgn Fluorescent reporter for serotonin (dendrite localization tag) Lin ...

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...insertions. When designing the donor template, incorporating mutations that prevent further Cas9 cleavage...Overview of HDR. In most applications, a large portion of the Cas9-induced DSBs will still be repaired...the desired HDR-edited allele. Therefore, it is important to confirm the presence of the desired edit experimentally...pairs to the 3’ flap, and the desired edit is incorporated into the DNA by reverse transcription. The edited...applications where orientation of the insert is important. However, type I-F CASTs favor insertion in the...

...necessary in cases where extreme specificity is important. Their epitope specificity allows binding to a...antibodies only bind a single epitope, so it is important to choose a monoclonal antibody that will recognize...using a secondary against a tag, such as His, incorporated in the sdAb. sdAbs can have weak signals due...with conjugating reactions are possible. It is important to research your reaction and buffer before conjugating... the basic steps needed for each application, important controls, and any special considerations to think...which are bacterial proteins that bind to the Fc portion of an antibody. However, they are not suitable ... assay, selection of the fluorophores used is important. It is best to inversely pair the brightness of...

...Collection The BRAIN Initiative supports the development of a diverse portfolio of biomolecular tools and emphasizes...The vast majority of cases (90–95%) of ALS are sporadic, having no prior family history. A small percentage...specific known cause) nature of the disease, a small portion of cases can be attributed to known genetic factors...This collection highlights plasmids created with support from the BRAIN Initiative. Jackson Laboratory (...a new window) . This resource was created with support from the Chan Zuckerberg Initiative (Link opens...

... cluster in the MBD and NID demonstrating the importance of binding to methylated DNA and the recruitment...syndrome at the molecular level. They are also an important tool for developing therapeutic strategies to ...MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell line (Link opens in a new window) PMID: 35148843...opens in a new window) MMRRC Repository - NIH-supported mouse and cell line repository (Link opens in ...Protocols.io - protocol sharing site Funding Opportunities (Link opens in a new window) Rett Syndrome Research... purposeful hand use in girls: Rett’s syndrome: report of 35 cases. Ann Neurol . 14, 471–479. (Link opens...

...drives expression of RFP as a reporter. See plasmid 17618 for GFP reporter. Linzhao Cheng 17452 pLenti ...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Luk...Expresses shRNA under mouse U6 promoter. CMV-EGFP reporter cassette is included to monitor expression. Stephan...86849 pBOB-EF1-FastFUCCI-Puro 3rd Encodes FUCCI reporters for cell cycle analysis. Puromycin selection. ...

...NOT Cre. B) This strategy has been expanded to incorporate VCre in order to enable three-feature cell targeting...recognition sequences enables directional control of the portion of the ORF that is sandwiched between the sites...working with INTRSECT recombinases, viruses, and important controls to consider as part of experimental design..., Poulin JF, Ansorge M, Awatramani R, Kang UJ, Rayport S. 2018. Dopamine neuron glutamate cotransmission... a new window) Mingote S, Amsellem A, Kempf A, Rayport S, Chuhma N. 2020. Dopamine-glutamate neuron projections...

...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified...agreement No 777372. This Joint Undertaking receives support from the European Union's Horizon 2020 research...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...knockout library targeting human transmembrane transporters. Feedback Please send your feedback to the Resolute...

...Addgene assists importation by including thorough shipping documents and following import procedure, it ...it is the responsibility of the importer to pay for any additional customs fees or taxes, and to complete... that might be required by your country for the import of biological research materials. Please be aware...

...Endogenous Zebrafish Gene Targets Recent published reports have shown that engineered zinc finger nucleases... making targeted mutations in zebrafish. With support from the NIH and guidance from the academic community...Nuclease Expression Vectors for more details). IMPORTANT NOTE: All zinc finger arrays listed show transcriptional...greater in a well-characterized bacterial two-hybrid reporter system but have NOT yet been tested for activity...

...induced reprogramming to pluripotency. Stem Cell Reports. 2015 Apr 14;4(4):727-43. Woltjen Plasmid Mouse...differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl...self-regulating and nonintegrating viral vectors. Stem Cell Reports. 2014 Nov 6. pii: S2213-6711(14)00307-5. Jaenisch...Cells and B Lymphocytes to Neural Stem Cells. Cell Reports. 2014 Dec 11;9(5):1673-80. Parmar Fibroblasts Astrocytes...astrocytes by defined transcription factors. Stem Cell Reports. 2015 Jan 13;4(1):25-36. Broccoli Fibroblasts Sensory...

...(sodium/bile acid cotransporter family), member 2 ASBT, ISBT, NTCP2 TAP1 transporter 1, ATP-binding cassette...grouped into tables based on the Gene Lists from Immport . Click the links below to go to the gene table...perforin and granzyme B, two enzymes that create pores within an infected cell membrane, triggering cell...polypeptide FCRG FCGRT Fc fragment of IgG, receptor, transporter, alpha FCRN, alpha-chain HFE hemochromatosis ...FLJ41500, PSF1, RING4, TAP1*0102N, TAP1N TAP2 transporter 2, ATP-binding cassette, sub-family B (MDR/TAP...ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin ADIPOR1 adiponectin receptor 1 ACDCR1, CGI-45, CGI45, FLJ25385... FLJ25385, FLJ42464, PAQR1, TESBP1A ADIPOR2 adiponectin receptor 2 ACDCR2, FLJ21432, MGC4640, PAQR2 ADM...

...editing, empty backbones, fluorescent proteins, reporters, biosensors, and Cre expression plasmids. Large...studying diseases, are listed below: Lipid droplet reporters - Richard White Lab. An imaging platform to study...controlled Cre/loxP system that enables precise temporal and spatial control of gene expression in zebrafish...using red light. LipoGlo - Steven Farber Lab. A reporter system that allows direct assessment of lipoprotein...

...single-nanometer scale, scientists can use FRET to report on protein binding or conformational changes. Organic...our Biosensors Collection to find fluorescent reporters for metal ions, small molecules, and more! Popular...your experiment, be sure to refer to published reports and include appropriate controls to help interpret...Grefen Lab. FRET pairs and biosensors can also incorporate organic fluorophores via HaloTag or SNAP-Tag ...

...Browse plasmids supporting the evaluation of a diverse set of novel Alzheimer's disease targets....TREAT-AD centers are focused on diversifying the portfolio of drug targets in Alzheimer’s disease (AD) by...molecules for prioritized targets that are valued opportunities for further translational investment. All resources...

...whole range of expression levels for these two reporters simultaneously. By fusing sfGFP and/or mCherry...cross‐talk. (A) Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in...phage attachment sites. The levels of the two reporters, mCherry and sfgfp in this case, can be controlled...

...with EF1alpha addition of an mCherry reporter variant nuclear reporters via an H2B fusion shuttle plasmid...advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several ...

...research opportunities to their students and other members of the MCC community. They support educators...local hub of educators teaching MDH CUREs, and opportunities to mentor future MCC members. Find a regional...

...control) none Constitutive 1, 9 Yulong Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Archon1 EGFP Cre dependent 8 Edward Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...-ST none Cre dependent 1 GENIE Project Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ...none Constitutive 9 Francois St-Pierre Voltage Reporter: ASAP 225707 pAAV-hsyn-ASAP5-Kv2.1-WPRE Syn ASAP5...

...elegans genome is well studied and hundreds of reporters have been developed and characterized which allow...for building homologous repair templates that incorporate a self-excising drug selection cassette (SEC)... elegans. - Jane Hubbard Lab. Plasmids for the temporal and spatial control of gene expression in C. elegans...

...114407 NUP153-mEGFP AICSDP-50 mEGFP Nucleoporin Nup153 Nuclear pores 114410 FUS-mEGFP AICSDP-64 mEGFP RNA-binding... from the Allen Institute for Cell Science data portal (Link opens in a new window) , including: data ...