We narrowed to 1,042 results for: URE

...endogenously-tagged cellular structures. Addgene ID Plasmid Allen Institute ID Tag Protein Structure 87420 PXN-EGFP ...fluorescently tagged proteins of key cellular structures in human cell lines. Depositor...fluorescently tagged proteins of key cellular structures in human cell lines of their choice. For a full...

...amplification, and high-quality quality control measures such as titering, purity assessment, sterility... comprehensive quality control processes to make sure it is ready for your experiments. Shipping 2–7 days...globally connected scientific community. The Data Hub captures valuable knowledge that often goes unshared, bridging...including purification, titration, and quality control measures. We have distributed preps around the globe and...

...and 63AA, respectfully). This next generation architecture has been shown to increase mutation induction... enables the evaluation of TALEN activities in cultured cells. Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate...systems including hard-to-transfect cell lines. Features include (i) Esp3I (BsmBI) restriction sites for...cells, (iv) an improved, truncated TALE backbone architecture as established by Miller et al. (PMID: 21179091...

...are primarily used for determining protein 3D structure and crystallography.... that aims to determine the three dimensional structures of proteins of medical relevance, and place them...empty backbones/controls) ID Plasmid GenBank Key Features 26092 p15TV-L EF456736 Hexahistidine tag with ...

...Addgene works with leading scientists in the field to ensure our CRISPR collections and resources are up-to-...below by function. Addgene's CRISPR collections feature plasmids for a variety of organisms, including ...fused to an epigenetic modifier alters chromatin structure/gene expression. RNA Targeting RNA Targeting Type...

...order. Experimental Disclaimer Due to the novel nature of experiments carried out with our materials, ...function of any viral vector. Addgene performs QC measures which verify the identity of the viral vector,...thorough shipping documents and following import procedure, it is the responsibility of the importer to pay...

...biology resources available at Addgene. Browse featured collections or depositing labs....Bacterial Plant Yeast Mammalian Worm Fungal Algal Featured SynBio Deposits Alper Lab Y. Lipolytica Promoters...papers on Syn Bio SEVA - Standard European Vector Architecture Open Plant - Plant SynBio resource Synthetic...

... into mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected...Plasmids The table below highlights plasmids that feature expression of antibodies, nanobodies or ScFvs. ...

...use light produced from an enzymatic reaction to measure subtle expression changes with high sensitivity...window) is an energy transfer technique designed to measure molecular proximity in living cells. NanoBRET PPI...protein. The apparent affinity of test compounds is measured by competitive displacement of a NanoBRET tracer...

...These mutations can be inherited or arise through exposure to mutagens like radiation or viruses (e.g., HPV...Thompson T, Karsenty G, Bradley A, Donehower LA. Nature. 2002 Jan 3;415(6867):45-53. PubMed PMID: 11780111...Ogryzko V, Gardner K, Giordano A, Levine AS, Kelly K. Nature. 1997 Jun 27;89(7):1175-84. PubMed PMID: 9215639...

...gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols, 2016 . Yamamoto ...very rapidly after the addition of auxin to the culture medium. Plasmids can be found associated with the...

...plasmid and the effects on gene expression can be measured by the luminescence output. The most popular types...substrate must be delivered intracellularly to measure luminescence. Gaussia luciferase is secreted and...altered emission, substrate use, stability, or other features. AkaLuc : Engineered to use the substrate Akalumine...

...-expressing BirA biotin ligase, as seen in the CAPTURE system . The locus is subsequently isolated by ...associated with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using...

...found in bacteria and archaea. While abundant in nature, Class 1 systems are largely underutilized compared...many still separate the individual Cas components. Figure 1: Overview of Cascade-Cas3 mechanism. Created ...

... Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection...

...regulation and developmental studies. Key plasmid features and pX458 modifications: replacement of the Cbh...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...

...2020 research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...is embedded in an integrated knowledgebase that features, among others, genetic variants and disease associations...

...for ammonium uptake. Nature, 446 (7132):195-8. https://doi.org/10.1038/nature05579 (Link opens in a new...

...shRNA design and delivery, see resources below. Figure 1: Overview of shRNA-mediated RNA interference....silence: conditional systems for RNA interference . Nature Methods, 3 (9), 682–688. PubMed (Link opens in ...

... change. Adenine DNA deaminases do not exist in nature, but have been created by directed evolution of...to activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort...

...driver for brain circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across...high-resolution optical imaging down to individual synapses. Figure 2: PRISM workflow. A) Stochastic infection of pooled...

...for distribution to the research community. Key features: Allows for visualization of multiple proteins...these tags. Plasmids Addgene ID Plasmid Substrate Feature 101137 pSNAP-tag (T7) Vector SNAP-tag Empty backbone...

...genome integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral...plasmids). When transfected into in vitro cell cultures, these plasmids direct the production of viral...

... Distribution to Industry Featured Collections Search Plasmids Plasmids Kits Pooled...Libraries Antibodies Ordering and MTA Information Featured Collections NEW NanoLuc and HaloTag Fusions from...

...GTPase signaling activity. Figure 1: Locations of ARF superfamily members. Figure from Szutl, et al., 2019...

...transparency of the embryo and larvae, it is easy to culture large populations that are amenable to genetics...combinatorial and cumulative genome editing - Jay Shendure Lab Simultaneous single-cell profiling of lineages...

...Collection (Link opens in a new window) Springer Nature SARS-CoV-2 and COVID-19 Articles (Link opens in... F. (2020). The proximal origin of SARS-CoV-2 . Nature medicine, 26 (4), 450–452. https://doi.org/10.1038...

...interest to create a luciferase reporter Promoter Measure promoter strength pBV-Luc - Luciferase ... vectors) pCAP03-acc(3)IV - Facilitate the capture and expression of bacterial gene clusters S. cerevisiae...

...Center at Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. ...Information When using the Caltech serotypes in future publications, please acknowledge Viviana Gradinaru...

...dynamic picture of the brain generated through this effort will provide novel ways to treat, cure, and even...