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We narrowed to 642 results for: ato

Showing: 31 - 60 of 642 results

  1. Chemogenetics AAV Preps

    Type
    Collection
    ...nEF CAG E2 regulatory element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity ...83896 pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hM4D(Gi) - Inhibition NLS-dTomato none 1, 9, rg* Fishell 83897...83897 pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Fishell 50472...Deisseroth 135635 pAAV-S5E2-Gq-P2A-dTomato hM3D(Gq) - Activation dTomato (physically separate) none 1, 9,...

  2. CRISPR Guide

    Type
    Collection
    ...effective gene activators when fused with dCas9. Recently, synthetic CRISPR-Cas gene activators have been ...zinc finger nucleases (ZFNs) or transcription-activator-like effector nucleases (TALENs) required scientists...fusing dCas9 to transcriptional repressors or activators and targeting promoter regions. You might sometimes...mice and human cells. The simplest dCas9-based activators and repressors consist of dCas9 fused directly...directly to a single transcriptional activator (e.g. VP64) or repressor (e.g. KRAB; Figure 9A). Other examples...co-expression of epitope-tagged dCas9 and antibody-activator effector proteins; long-term imaging of proteins...instead of a two-component system (Figure 9C) SAM activators - co-expression of dCas9-VP64 with a modified...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...mutations of these regulatory elements on gene expression. Empty backbones for inserting regulatory elements before...are commonly used to investigate the effect of regulatory elements, such as promoters, enhancers and untranslated...Optimized STARR-seq ( S elf- T ranscribing A ctive R egulatory R egion) plasmids : Genome-wide screening of enhancer...cloning to test for the presence of transcriptional regulatory region in specific cell types. Used to identify...backbone plasmids into which you can clone your regulatory element or gene of interest into to create a ...Lentiviral expression of firefly luciferase and dTomato. A gene of interest can also be inserted into the...Reporter Constructs Already know what gene or regulatory element that you need a luciferase reporter for...

  4. Plasmids for Stem Cell Research

    Type
    Collection
    ...Fibroblasts Hepatocytes Retroviral Mouse Direct conversion of mouse fibroblasts to hepatocyte-like cells.... 2016 Mar 7;7:10869. Hu CRISPRa Human CRISPR activator system for reprogramming human cells to pluripotency...genes Human pluripotent reprogramming with CRISPR activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski ...generation OCT4 and SOX2 Work as Transcriptional Activators in Reprogramming Human Fibroblasts. Cell Rep....2013 Apr 13;60C:97-106. Gearhart Fibroblasts Hematopoietic Progenitor Cells Lentiviral Mouse Direct reprogramming...reprogramming of murine fibroblasts to hematopoietic progenitor cells. Cell Rep. 2014 Dec 11;9(5):1871-...1871-84. Lacaud Fibroblasts Hematopoietic Progenitor Cells Lentiviral Human Cooperative Transcription Factor...

  5. Biosensor AAV Preps

    Type
    Collection
    ...-nls-dTomato EF1a GCaMP6f dTomato Cre dependent rg* Ting 51085 AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Syn GCaMP6f...Rose 51082 AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato EF1a GCaMP6s dTomato Cre dependent 1 Ting 105714 pAAV-Ef1a-fDIO-GCaMP6s...Larsen 51084 AAV-hSyn1-GCaMP6s-P2A-nls-dTomato Syn GCaMP6s dTomato Constitutive 1, rg* Ting 68717 pAAV-CAG-Flex-mRuby2...CAG CaMKIIa Dlx EF1a GFAP/GfaABC1D Synapsin E2 regulatory element Activity Cre-dependent Flp-dependent ... GCaMP6f dTomato Constitutive 1, rg* Ting 52924 pZac2.1 gfaABC1D-lck-GCaMP6f GfaABC1D GCaMP6f none Constitutive...

  6. Rett Syndrome

    Type
    Collection
    ...NLucTom Knock-in of NLuc-tdTomato at endogenous MECP2 locus Castaneus MECP2-NLuc-tdTomato mouse reporter cell... working with the RSRT along with individual laboratories to assemble a Rett Syndrome plasmid resource...The MECP2 protein is a global transcriptional regulator of thousands of genes and studies have suggested...contains a Nuclear receptor Co-Repressor 1/Silencing Mediator of Retinoic acid and Thyroid hormone receptor ...contact information by following the link to their laboratory website. Mouse Line Mutation (DNA) Background...contact information by following the link to their laboratory website. Cell Line Mutation (DNA) Mutation (protein...prenatal LMD microarray data, ISH image data, and anatomic reference atlases of prenatal and adult human ...

  7. CRISPR References and Information

    Type
    Collection
    ...Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode: Injection... dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression... Lab and maintained by the Frederick National Laboratory at the National Cancer Institute. Cas-OFFinder...pAC91 , pAC92 , pAC93 , pAC94 , pAC95 ; dCas9 activator gateway donors: pAC84 , pAC1 , pAC147 , pAC148...; Gateway destination: pAC90 PDF, 1.1 MB Katic Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone...

  8. mTOR Pathway

    Type
    Collection
    .... enhanced signaling of mTOR, a key metabolic regulator, correlates with poor prognosis in many cancers...arget o f r apamycin (mTOR) is a key metabolic regulator controlling cell growth and proliferation... caused by inactivating mutations in negative regulators of mTORC1. The hyperactivation of mTORC1 in cancer...Ras-related GTP binding Raptor Also known as RPTOR; Regulatory associated protein of MTOR, complex 1 Rheb Ras...Catalytic Subunits PIK3CA PIK3CB PIK3CD PIK3CG Regulatory Subunits PIK3R1 PIK3R2 PIK3R3 PIK3R4 PIK3R5 PIK3R6...Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic and regulatory subunits - Class I PKC PKC alpha PKC beta PKC...

  9. Working with Nuclear Receptors

    Type
    Blog Post
    ... for a curated list of coregulators). Coregulators can function as coactivators and corepressors depending...complexes with secondary coregulator (or co-coregulator) partners. The coregulator ‘core’ complex is composed...feature afforded by coactivator complexes suggests that coactivators may be integrators of multiple cell ...illnesses, and the metabolic syndrome. Coregulators Coregulators constitute a group of some 400 molecules...that coregulators belong to distinct complexes may explain how more than 300 different coactivator proteins...manner. Because coregulators exist as multiprotein complexes, a member of a single coactivator complex can... W. (2002). Combinatorial control of gene expression by nuclear receptors and coregulators. Cell 108, ...

  10. Worm Expression Resources

    Type
    Collection
    ...Constructs Kits and Collections Other Resources The nematode Caenorhabditis elegans ( C. elegans or “the worm... approach has also been developed by the Fire laboratory and described in Efficient Marker-Free Recovery...contains 29 plasmids used to generate transgenic nematodes, including C. elegans and C. briggsae. Other related...A database featuring behavioral and structural anatomoy of C. elegans. WormBase - A consortium of biologists...genomics and biology of C. elegans and related nematodes. WormBook - A comprehensive, open-access collection...related to the biology of C. elegans and other nematodes. Wormbuilder - Describes methods to engineer the...

  11. Lentiviral Prep Service

    Type
    Collection
    ...dCas9) can be fused to a transactivator and used as a transcriptional activator . ID Name Insert Antibiotic...dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A GFP Zhang 61425 lenti dCAS-VP64_Blast ...Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal...

  12. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    ...Choose a moderator with a backbone. It is nice to have a subject area expert, but the moderator is not ...LinkedIn profiles of all panelists to the moderator. The moderator and organizers should develop a list of...(lapel mics are best). The moderator takes control It is the moderator's job to prevent anyone from ...effortless to the audience, but the organizers and moderator should have a well-planned approach to making ... of 4-6 pre-event interview questions. The moderator (possibly with an organizer) should set up a 30 minute...to schedule. In the pre-event interviews, the moderator should be taking good notes and discovering interesting...opinions that each panelist can contribute. The moderator should identify the funny, provocative or interesting...

  13. TALEN Engineering

    Type
    Collection
    ...Reagents from the Keith Joung laboratory for engineering TAL effectors, including designed TALENs, and..., Nat Biotechnol. 2011 ) TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013...dimeric TALEN and monomeric TALE transcriptional activator target sites and to simplify the construction ...interest for potential TALEN or TALE transcriptional activator target sites and then generates individual user-friendly...REAL, REAL-Fast and FLASH TALE Transcriptional Activator Expression Vectors for REAL, REAL-Fast and FLASH...

  14. Deisseroth INTRSECT Collection

    Type
    Collection
    ...fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium indicators, and rabies targeting...INTRSECT (intronic recombinase sites enabling combinatorial targeting) is a synthetic molecular targeting...doubly-specified combination of genetic and/or anatomical-defined parameters, by placing two orthogonal...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...

  15. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... the perfect voltage indicator and optogenetic actuator pair. Ideally, indicators should possess fast ...lab wished to improve on the Arch indicator and their collaborators in Robert Campbell’s lab carried out...kinetics. By combining both the voltage indicator, QuasAr, and actuator, CheRiff, in the bicistronic Optopatch...activation of specific genes using dCas9-VP64 activator fragments (dCas9(C)-FKBP-2xNLS-VP64 and dCas9(...62885 & 62886) Rapamycin-inducible dCas9-VP64 activator set (Addgene plasmids 62887 & 62888) Zetsche... which will ultimately co-localize the VP64 transactivator with a dCas9 that has been targeted to a very...tool for activating endogenous genes Moritoshi Sato's Lab has engineered CRISPR-Cas9 to create a photoactivatable...

  16. Synthetic Biology - Overview

    Type
    Collection
    ...and synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also... Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and Orthogonal Switches...

  17. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Roth New serotype pAAV-EF1α-F-FLEX-mNaChBac-T2A-tdTomato AAV8 Molecular tool Massimo Scanziani New viral...Optogenetics Jonathan Ting New viral prep pAAV_BiSSTe10_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiCHATe27_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiVIPe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Gordon Fishell New viral prep pAAV_BiLAMP5e3_dTomato_nlsdTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe4_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...Optogenetics Gordon Fishell New viral prep pAAV_BiPVe3_dTomato_nlsdTomato AAV-PHPeB Control Gordon Fishell New...

  18. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...cheaper to use Cas9 activators. This larger scale of activation allows for exploratory-based research as...of the gene, Cas9 activators efficiently solve these problems.   Which Cas9 activator should I use? There...significant work on constructing Cas9 transcriptional activators. These constructs allow for the upregulation ...genome-wide studies One of the best uses for Cas9 activators is in genetic screening. gRNAs targeting every... using oligo library synthesis. Prior to Cas9 activators, similar tools were made using other DNA binding...to easily change the sequence targeted by the activator by simply providing a new gRNA rather than engineering...organism, have been used in a similar manner to Cas9 activators. However, these can be difficult to construct...

  19. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Repressible: An activator protein is bound, turning transcription ON. When a repressor binds the activator protein...introduced the second generation QF2 activators. These activators solve the problem of broad QF expression-induced...protein, the activator protein cannot bind the promoter sequence anymore and transcription is turned OFF....protein, the repressor protein can bind to the operator. The bound repressor then prevents transcription... repressor (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In... Bujard created the tetracycline-controlled transactivator (tTA). They fused TetR with the transcriptional...by Gossen and Bujard, both the promoter and transactivator components of the system have been engineered...

  20. p53 Pathway

    Type
    Collection
    ..., which disrupt its interaction with negative regulators, increase its stability and DNA binding activity...activity, and allow it to bind transcriptional co-activators and modulate transcription. The list of p53 transcriptional...proto-oncogene, E3 ubiquitin protein ligase MDMX p53 regulator; also known as MDM4 Noxa Phorbol-12-myristate-...peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis...binding component 3 Reprimo TP53 dependent G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins...

  21. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... of robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the Church...method, called STARR-seq (self-transcribing active regulatory region sequencing) that tests enhancer sequences...promoters, RBS’, coding sequences (CDS), and terminators of varying strengths. These can be easily combined...both standard Gateway cloning and the FNLCR Combinatorial Cloning Platform (CCP); the latter of which ...pooled libraries, but thanks to a deposit from the laboratory of Dr. Ji-Long Liu at Oxford University, we are...and context-dependent human fitness genes, the laboratory of Jason Moffat at the University of Toronto ...effectiveness of their library design, Moffat laboratory members conducted multiple screens in different...

  22. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ... pFA6a-link-tdTomato-SpHis5 - Yeast Expression tdTomato-N1 - Mammalian Expression tdTomato-C1 - Mammalian... Bacterial Expression tdTomato 554 581 95 4.7 1 hr Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...Mammalian Expression tdTomato-pBAD - Bacterial Expression Jump to Top Red Protein Excitation (nm) Emission...

  23. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...the activator - the CRY2 photolyase homology region (CRY2PHR) fused to a transcriptional activator domain...control transcription. Two separate labs, Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach...directed by the guide RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the figure below...and CIB1 proteins heterodimerize and move the activator into position to activate gene transcription. ...fusion of full-length CRY2 and the transcriptional activator domain VP64. Using this system in HEK293T cells...NHEJ and HDR using split systems Later in 2015, the Sato lab unveiled a photoactivatable system to cleave...DSBs (non detectable). Like their prior work, the Sato lab also showed that the paCas9-2 system could be...

  24. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...either the optogenetic activator channelrhodopsin-2 or fluorescent reporter dTomato under the control of...Here's what you'll find in this post: Glutamate indicators with increased sensitivity and tailored deactivation...viral preps targeting interneurons Glutamate indicators with increased sensitivity and tailored deactivation...imaging rapid synaptic events such as in the somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise..., Iyer, R., … Podgorski, K. (2025). Glutamate indicators with increased sensitivity and tailored deactivation...Glypicans By Ashley Waldron Glypicans are important modulators of cell signaling pathways during embryonic development...Shilatifard Lab was able to identify both new and known regulators at the FOS gene promoter and the MYC locus (Figure...

  25. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...Suntag” Cas9 activator that recruits up to twenty copies of the p65-HSF1 transcriptional activator to each ...and engineered combinations of transcriptional activators to induce gene transcription at genomic sites... vitro settings due to the large size of Cas9 activators, which largely precludes delivery by adeno-associated...will fit). Cre-dependent expression of the SPH activator paired with AAV sgRNA delivery make the SPH mouse...calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters...Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by lentivirus, which...expression of dCas9-VPR (Figure 2), a popular Cas9 activator developed by the Church Lab. Neuron-specific expression...

  26. NETRF

    Type
    Collection
    ...from the NETRF-funded investigators listed below. Depositing Principal Investigators (PIs) PI Institution...Shivdasani Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...Tumors Qiao Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors...

  27. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...editing assemblies are sensor-actuators, either transcriptional regulators or chromatin-modifying enzymes...s chromatin status into account (Fig 1a). The laboratory of Karmella Haynes at Emory recently contributed...with examples of potential synthetic chromatin mediators and probes. Image from Priode et al., 2021. ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying...interactions By Jennifer Tsang GFP-based Touching Nexus (G-baToN) is a new tool developed by Rui Tang in Monte Winslow...label cells after cell-cell interactions. Using G-baToN, “sender” cells express GFP on the surface and “...receiver cell. Image from Tang et al., 2020.   G-baToN can also be applied to other fluorophore/antibody...

  28. CRISPR Plasmids - Activate Gene Expression

    Type
    Collection
    ...gRNA sequence to direct the dCas9-activator to promoter or regulatory regions of your gene of interest....Catalytically dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific...separate gRNA expression plasmid to target the dCas9-activator to your specific locus. Want more information ...

  29. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...single-molecule biophysics Fast and responsive voltage indicator ONE-GO biosensors kit Antibodies for neurodegeneration...lab recently "broke the PE efficiency barrier in tomatoes" by combining several approaches (Vu et al., 2024...chaperone, RT: reverse transcriptase. Right: T1 tomato plants that inherited an edited gene to confer ...the team was able to drive prime editing in both tomatoes and Arabidopsis, suggesting the improvements may... species. In addition, they generated offspring tomato plants that inherited the genomic edit but not ...prime editing enables heritable desired edits in tomato and Arabidopsis. Nature Plants, 10(10), 1502–1513...2024.12.20.629623.  A fast and responsive voltage indicator with enhanced sensitivity for unitary synaptic...

  30. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...the LEGOs were mirrored by the nearly infinite regulatory capabilities of gene pathways just waiting to...was creating a LEGO stage that resembles a real laboratory. It only took one night to build the first model...use our imaginations to mold it all into a full laboratory. It is priceless to see our “toys” gaining life...working as scientists in a custom made, colorful laboratory. When we started, the hardest part of the process...building each scenario, I go “WOW, now I have my own laboratory” (the main character is much more than a piece...
Showing: 31 - 60 of 642 results