We narrowed to 988 results for: igh

...neuroscience -related antibodies. Find antibodies to highly conserved proteins and other difficult-to-target...and scFvs for neuroscience research created with high-volume hybridoma sequencing on the NeuroMabSeq platform...dissemination to the research community. This collection highlights plasmids created with support from the BRAIN ...

...mutations are known to cause Rett syndrome, however, eight recurrent missense and nonsense mutations (R106W... labs Plasmids MECP2 Plasmids The table below highlights plasmids that express or target the MECP2 gene... 55–67. PMID: 30536762 Shahbazian et al. 2002. Insight into Rett syndrome: MeCP2 levels display tissue...

...vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution imaging...screened thousands of enhancers for specificity and brightness of fluorescent labeling across brain regions ...

...the lowest biosafety protection levels. High quality and high titer in-stock AAV preps are affordable,... density centrifugation. Addgene’s comprehensive high-quality quality control process includes titering...

...luminescent opsins: fusion proteins of a light-emitting luciferase and a light-sensing optogenetic element such...oxidizes its substrate (luciferin), it generates light that activates the opsin to either excite or inhibit...

...We provide high quality AAV preps from select plasmids in the repository. Browse our AAV collection of... Service: Recombinases Cre AAV Dre AAV Flpo AAV Light-Inducible Recombinases VCre AAV Browse the following...TBG none 8 Melina Fan , Addgene Research Program Light-Inducible Recombinases ID Name Promoter Fluorophore...

...joining fragment comprising a κ light chain constant domain (C L ) and the κ light chain polyA tail sequences... for light chain expression, and an ER signal/leader sequence (L) for translocation of the light chain...in a new window) Durocher Y, et al. 2002. High-level and high-throughput recombinant protein production... R-mAbs are secreted into the culture media. For high level expression we recommend using the HEK-2936E...Link opens in a new window) . The schematic to the right shows the separate elements of the R-mAb expression...expression plasmid involved in coexpression of light (green) and heavy (blue) chains as driven by two CMV promoters...

... for each specific antibody in order to generate high quality preparations. Following production, all ...transfected with the plasmid(s) encoding the heavy and light chains. Antibodies are expressed for 7 days and ...amplified with primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified...

...— contains packaging gene env This collection highlights popular gamma-retroviral transfer, packaging,...Foijer 47916 pRXTN CMV/MSV A modified version of pRX-tight Puro encoding NAT instead of PAC. James Iglehart...

...Core) and Addgene previously partnered to provide high-quality AAV vectors to the academic research community...Deisseroth AV-1-36857M 100038-AAV1 pENN.AAV.hSynap.Flex.ArcLightDco.WPRE.SV40 Vincent Pieribone AV-1-36857P...36857P 100037-AAV1 pAAV.hSynap.ArcLightD.WPRE.SV40 Vincent Pieribone AV-1-51473 51473-AAV1 pAAV-hSyn1-mRuby2...James M. Wilson AV-1-PV2699 100030-AAV1 pAAV.CBA.loxP.ArcLightD.2A.nlsmCherry.loxP.WPRE.SV40 Lawrence Cohen...

... are able to recognize their targets with fairly high specificity. Fifteen years of research has shown...TALe research has been swift. The incentives are high. Since ZF targets are confined to sequences composed...on specificity is on par with ZF arrays, if not slightly lower. Figure 2: Simplified representation of ...

...— contains packaging gene env This collection highlights popular second- and third-generation lentiviral...transgene expression. Contains intron that gives high expression. Didier Trono 12254 pWPI 2nd EF-1alpha...

... nLightR. A, Structural models of nLightG (left) and nLightR (right) generated using ColabFold (Mirdita...indicators: nLightG and nLightR (Kagiampaki et al., 2023).     Figure 1: Properties of nLightG and nLightR...al., 2023. nLight nLight sensors are based on alpha-1a adrenergic receptor; nLightG contains circularly...norepinephrine. nLight is 28-fold more selective than GRABNE, with both nLightG and nLightR showing significantly...experimental design. Find nLight plasmids here! Next steps While nLightG and nLightR greatly expand the toolbox... both nLightG and nLightR, and may soon have a red tool which matches the sensitivity of nLightG. Many...images of HEK293T cells and neurons expressing nLightG or nLightR before/after application of NE (10 μM) and...

...pAAV-EF1a-iCreV EF1a light-inducible recombinase iCreV Zeng 140136 pAAV-EF1a-iDreV EF1a light-inducible recombinase...recombinase iDreV Zeng 140137 pAAV-EF1a-iFlpV EF1a light-inducible recombinase iFlpV Zeng 164450 CN1851-rAAV-hI56i-minBglobin-iCre...

...Migration Consortium (CMC) and Addgene, which highlights CMC depositing labs and plasmids....

...resources for emerging targets in AD and creating high-quality, well-characterized lead molecules for prioritized...further translational investment. All resources and high-quality research tools are shared openly. Plasmids...

...a variety of popular plasmids and backbones with high cloning potential. By making these plasmids available...spectrophotometer. Other methods of quantification may yield slightly different DNA concentrations. Can I directly transfect...

...the results, techniques, and reagents with the highest-possible quality of research output, RESOLUTE expects...production is used for assays and to generate antibody/ high-affinity ligands. Data is embedded in an integrated...

...Addgene determines that our current queue is too high and we do not expect to meet the general turnaround...complete any additional customs documentation that might be required by your country for the import of biological...

...used for gene delivery due to their high packaging capacity, high efficiency in driving strong transient...

...pre-assembled genetic circuits such as logic gates and higher level gene networks. Sensing and Signaling Browse...Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource Allocator and...

...Method Full Target Site (5' to 3') Left half-site Right half-site CACNA1F OZ507 and OZ508 OPEN OPEN gCGTGACCTCgctgatAAGAAGAGg...engineering of customized zinc-finger nucleases for highly efficient gene modification. Maeder ML et al. Mol...

... gRNA vectors suitable for prime editing are highlighted in the table below. Use the search bar to find...

... 87429 TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP...

...and C terminus and induces mutation at rates much higher than the parental vectors. pC-GoldyTALEN directs...obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite vectors. 37276...

...Advantages Representative Empty Backbones AAV High transduction efficiency, but do NOT integrate into... Retroviral backbones made by Addgene Adenoviral High transduction efficiency, but do NOT integrate into...

...engineering using the CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . ... engineered CRISPR-Cas9 complex. Konermann S*, Brigham MD*, Trevino AE, Joung J, Abudayyeh OO, Barcena...

...Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP HTT CFP TREtight Huntington's Nico Dantuma 23971 ...Alzheimer's Gavin Wright 51643 APP-bio-His APP His, CD4 CMV Alzheimer's Gavin Wright 51811 PRNP-bio-His...PRNP-bio-His PRNP His, CD4 CMV Dementia Gavin Wright 53363 pCB268 APP Flag, HA, Myc CMV Alzheimer's Alexander Varshavsky...132389 AAVS1 CAG rtTA3 TauWT 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 132393 AAVS1...AAVS1 CAG rtTA3 TauP301L 2N4R-EGFP MAPT GFP TREtight Parkinson's, FTD Gerold Schmitt-Ulms 133320 pHBS834...

...based on using a thin plane (sheet) of light generated by sunlight to observe single gold particles with...V= ventral Developments in light sheet fluorescence microscopy Modern light sheet fluorescence microscopy...out-of-focus reflected light by using a pinhole.  However, the excitation light excites all of the fluorophores..., there is a drop in image brightness with increasing depth caused by light scattering and absorption....of this century has seen some major advances in light microscopy, particularly related to the neurosciences....  One of these advances in microscopy has been light sheet fluorescence microscopy (LSFM). The underlying...a fluorescent sample and captured the reflected light using a different objective lens oriented perpendicular...

...for many years (at a very low salary I might add). You have a right and responsibility to choose a lab where...Why is choosing the right lab such a big deal? It’s actually something you CAN choose and it will make...difference for your future career and life. You might see a lab head as choosing you, but in reality, ...communication before making this important decision. I’ll highlight some of these questions in the sections below....you are good at asking for and getting help, size might not be a big factor for you. Know who's boss You...considering a career at a small, teaching college you might want to consider less expensive, portable areas ...ownership" and author order PI lets lab members fight it out PI intervenes if there are interpersonal...