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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 386 results for: nans

Showing: 31 - 60 of 386 results

  1. Tags and Other Markers

    Type
    Collection
    ...Find recombinant antibodies targeting epitope tags and common fusion proteins....common cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

  2. Neuroscience

    Type
    Collection
    ...Find recombinant antibodies targeting neuroscience-related proteins....in neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

  3. Plasmids for Stem Cell Research

    Type
    Collection
    ...Otonkoski Lentivirus Human Expression of human Sox2, Nanog, Oct4, and Lin28 from four separate lentiviral plasmids...polycistronic expression of human Oct4, Klf4, Sox2, c-Myc, Nanog, Lin28, NR5A2, and microRNA 302/367 in three different... gene-specific plasmids at the following links: NANOG , OCT4 , SOX2 , MYC , KLF4 , LIN28 . Differentiation...Methods. 2015 Nov 25. pii: S1046-2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN...

  4. Antibodies

    Type
    Collection
    ...Overview of Addgene's ready-to-use monoclonal recombinant antibody collection. These affinity reagents ... Antibodies Addgene distributes ready-to-use recombinant antibodies to help facilitate scientific research...affinity reagent plasmids - including antibodies, nanobodies, scFvs, and more! Find Plasmids...

  5. All Antibodies

    Type
    Collection
    ...Browse Addgene's collection of recombinant monoclonal antibodies....Addgene distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-...Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype Reactivity...

  6. Distribution to Industry

    Type
    Collection
    ...Ordering and MTA Information Featured Collections NEW NanoLuc and HaloTag Fusions from Promega COVID-19 SARS-... Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...as by our trusted partner labs. Learn More ID Recombinant Antibody Reactivity Recommended Applications ...

  7. Depositor Collections

    Type
    Collection
    ...Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection Disease Research Michael... Simpson Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project ...

  8. Brain Initiative Collection

    Type
    Collection
    ... Antibodies Addgene distributes ready-to-use recombinant antibodies made from plasmids in our collection...partner labs. Learn more about our antibodies ID Recombinant Antibody Reactivity Recommended Applications ...Initiative Resource List . NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection . The NeuroMab Facility...

  9. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...) are small viruses originally discovered as contaminants of adenovirus stocks. One major advantage of...low immune response and low toxicity. Although recombinant AAV does not integrate into the host genome, ...

  10. p53 Pathway

    Type
    Collection
    ...to activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget S, Khoury MP,...

  11. Viral Vectors

    Type
    Collection
    ...Stable RNA <8 Kb 80-130 Dividing Yes Moderate * Recombinant AAV has a low frequency of target cell genome... Lab Lentivector Toolbox Guide to Generating Recombinant Adenoviruses Eric Campeau's Viral Vector Guide...

  13. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...overall improved ease of use when expressing recombinant phosphoproteins. These attributes make rEcoli... opens in a new window) Robust Production of Recombinant Phosphoproteins Using Cell-Free Protein Synthesis...

  14. Resolute Plasmid Collection

    Type
    Collection
    ...partnership of 13 members from industry and academia financed by the Innovative Medicine Initiative (IMI) of...

  15. Mammalian RNAi Tools

    Type
    Collection
    ...for cancer therapy . International Journal of Nanomedicine, 18 , 7605–7635. PubMed (Link opens in a new...

  16. Synthetic Biology - Overview

    Type
    Collection
    ...Kit Optogenetics Pertz Lab cpFRET Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway...

  17. Microbiology Resources

    Type
    Collection
    ...MoClo : Modular cloning for applications like recombinant protein purification and cell-free circuit design...

  18. Worm Expression Resources

    Type
    Collection
    ...cycle (3 days from egg to adult) and easy lab maintenance. Worms are particularly well suited for studies...

  19. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... localization Optogenetics Background Förster resonance energy transfer, or FRET , is a process by which...

  20. Viral Production

    Type
    Collection
    ...viral genome and the absence of unexpected DNA contaminants. The data is also used to determine recombination...

  21. CRISPR Guide

    Type
    Collection
    ...Cian, A., Itier, J. M., Anegon, I., Lopez, B., Giovannangeli, C., & Concordet, J. P. (2018). CtIP fusion ...prime editing systems by manipulating cellular determinants of editing outcomes. Cell . 184 (22), 5635-5652...

  22. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    ...break down how different contaminants impact these ratios.  Protein contaminants Whether you are purifying...microvolume spectrophotometers, the Thermo Scientific NanoDrop is one of the most popular options, so most of...of this post is based on reading results from a NanoDrop. The principles will apply to similar instruments...Characteristic absorption spectra for pure DNA and likely contaminants found in DNA samples. The blue lines in B-D ...B-D represent the spectra for the specified contaminant, while the grey lines in B-D represent the pure ...pure DNA spectra for comparison. Each of these contaminants will have a slightly different impact on the ...to varying degrees across the UV range used in NanoDrop systems. Peak absorption occurs below 230 nm, ...

  23. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice express hybrid...Antibodies  Secondary Nanobody Toolbox No Llamas Required - Synthetic Nanobodies Against Membrane Proteins...single-domain antibodies (sdAb), commercially known as nanobodies (Arbabi-Ghahroudi, 2017)!      Figure... single-domain antibody, under the commercial "Nanobody" name.  We’ve talked about sdAbs a number ...alpacas). But because sdAbs are so amenable to recombinant expression (and because maintaining a llama facility...mice that express camelid variable domains (aka nanomice), which gives researchers the “best of both worlds... Jin B, Odongo S, Radwanska M, Magez S (2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics...

  24. Hot Plasmids - August 2020

    Type
    Blog Post
    ...160374 Nanobodies- there's a simple method for that! By Michelle Cronin The benefits of nanobodies over ...antibodies. Nanobodies are easily expressed in the cytosol of E. coli, however the nanobody proteins tend...evolution Fluorescent tools for mammalian cells Nanobody purification CRISPRi and CRISPRa system for hPSCs...protease used to cleave affinity tags from purified recombinant proteins at small and large scale.  This new ... conventional antibodies can’t be overlooked. Nanobodies are smaller in size, more durable, and usually...alleviates the inclusion body problem and makes nanobody production in E.coli as simple as following a ...signal peptide of MBP, followed by the desired nanobody (NB) sequence, an AviTAG, and 6xHis tag. The MBP...

  25. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...Variables (scFvs). Nanobodies Figure 3: Comparison of the Hcab and nanobody. Nanobodies are the variable...antibodies here. Recombinant antibodies Similar to monoclonal antibodies, recombinant antibodies are a...synthetic nanobodies, or synbodies, can be produced from plasmids, saving time and money. Nanobodies and synbodies...from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their sequences...to reach. While a full antibody is ~150 kDa, a nanobody is 12-15 kDa.   Research applications for antibodies...Collection includes plasmids that express antibodies, nanobodies, and ScFvs, as well as tools for developing and...Developmental Studies Hybridoma Bank Learn more about nanobodies Watch our Antibodies 101 animation, What is an...

  26. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...multicolor NIR imaging.  Small miRFPnano A separate class of NIR FPs, named miRFPnanos, were derived from cyanobacteriochrome...545 3.7 62 8 miRFP670nano3 645 670 129,000 18.5 387 Monomer 675 4.2 250 9 miRFP704nano 680 704 93,...to your camera.  Two-photon image of miRFP670nano3-expressing neurons in the neocortex of a living...Their advantages are their small size (17 kDa for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and ...the proximity of the N- and C-termini. Latest miRFPnanos9 do not yield to (m)iRFPs in cellular and molecular... 97 Monomer 510 4.5 117 12 CBCR-based FPs miRFP670nano https://www.addgene.org/127443/ 645 670 95,...93,000 9.9 149 Monomer 1265 4.1 81 miRFP718nano 690 718 79,000 5.6 72 Monomer 885 3.8 33 State-of-the-art...

  27. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins... engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...gracilirostris) Natural 455 19 Coelenterazine None Yes NanoLuc®(derived from Oplophorus gracilirostris) Engineered... (fluorescent and luminescent) worlds Forster Resonance Energy Transfer (FRET) is the transfer of energy...which then emits it as light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except...protein-protein interactions Split luciferases, such as NanoBiT, are expressed in two subunits and only catalyze...

  28. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    ...microenvironment’s malignancy but also incite different organs to become malignant. Exosomes also contain...on the conversion of non-malignant forms of cancer to invasive and malignant forms and noticed that myoepithelial...that when there’s less caveolin 1, there’s less malignancy because there’s less proliferation. However, ...exosomes are tools of communication and provide malignancy to the microenvironment itself. They deliver ...myoepithelial cells prevented progression to malignancy, concluding that these cells may be considered...PMC4788391. Kogure, Takayuki, et al. "Intercellular nanovesicle‐mediated microRNA transfer: A mechanism of environmental...Central PMCID: PMC3310362. Martins, Diana, and Fernando Schmitt. "Microenvironment in breast tumorigenesis...

  29. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...through the use of protein tags and separated from contaminants using a variety of purification methods. Deciding...expression systems are very powerful for high level recombinant protein expression. These systems enable high...cheap and low-tech means of mass expression of recombinant proteins. Many cells from various types of plants...modifications. Extraction and purification of recombinant proteins from plants however can be expensive...biochemicals and proteins through plant roots. Tagging recombinant proteins with a naturally secreted plant peptide...expression system to generate large quantities of recombinant eukaryotic proteins. Although many species of...using S. cerevisiae, researchers often place recombinant proteins under the control of the galactose inducible...

  30. AAV Vector Quality Control: Going the Extra Mile with NGS

    Type
    Blog Post
    ...serotype of the packaged DNA and look for potential contaminants. The analysis is a 2-step process using Geneious...total reads are most likely to correspond to the contaminant. Each contig is then BLASTed against the NCBI...contaminated. For AAV-68544, the best match for the contaminant is Addgene plasmid #44362. Method validation,... were easily able to identify AAV-44632, the contaminant we mixed into the original AAV sample at 5%. ...software looks for and quantifies the presence of recombinants in recombinase-dependent sequences. Recombinase-dependent...powerful tool that can be used to identify DNA contaminants in AAV preparations and provide information ...Encapsidation of Prokaryotic Sequences during Recombinant Adeno-Associated Virus Production and Their in...
Showing: 31 - 60 of 386 results