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Showing: 21 - 40 of 393 results

  1. Plan Your Experiment

    Type
    Collection
    ...that cleaves DNA heteroduplexes, and run on an agarose gel to identify DNA fragments. PCR and restriction...the appropriate restriction enzyme and run on an agarose gel to identify DNA fragments. PCR amplification...insertions only). The PCR product is then run on an agarose gel to determine whether the edit was successful...

  2. Zhang Lab CRISPR Page

    Type
    Collection
    ... microbial nuclease system involved in defense against invading phages and plasmids. CRISPR loci in microbial..., Scott DA, Weinstein JA, Ran FA, Konermann S, Agarwala V, Li Y, Fine EJ, Wu X, Shalem O, Cradick TJ, ... CRISPR-Cas9 system. Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F. Nat Protoc . 2013 Nov;8(...

  4. Viral Vectors

    Type
    Collection
    ... While plasmids are typically used to encode, propagate, and manipulate genetic information, viruses are...research typically do not have the ability to propagate in the same way that naturally occurring viruses...

  5. CRISPR Guide

    Type
    Collection
    ...systems that bacteria and archaea use to protect against these foreign nucleic acids. Acr family members...3' NNNNGATT Streptococcus thermophilus (ST) 3' NNAGAAW Treponema denticola (TD) 3' NAAAAC Additional Cas9s..., Weinstein, J. A., Ran, F. A., Konermann, S., Agarwala, V., Li, Y., Fine, E. J., Wu, X., Shalem, O., ...: 27984730 Ran, F. A., Hsu, P. D., Wright, J., Agarwala, V., Scott, D. A., & Zhang, F. (2013). Genome ...

  6. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...easily identify your protein using an antibody against that epitope. This could allow you to conduct...favorite gene even if you do not have an antibody against it. Another common scenario is fusing your...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Curr Biol. 2018 Feb 5;28(3):444-451.e6. Takeharu Nagai Magnesium MagFRET-1 Mg2+ sensor, based on Cerulean...polypeptide. Sci Rep. 2021 Aug 13;11(1):16519. Takeharu Nagai Temperature Fluorescent temperature indicator B-...Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu Nagai Tension TS module, Vinculin tension sensors Measuring...

  10. CRISPR Plasmids - Parasites

    Type
    Collection
    ...knockout gRNA pooled library designed with 10 guides against 8,158 predicted protein-coding genes. Do you have...

  11. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ... Pseudotyping Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein...

  12. Antibody Production

    Type
    Collection
    ...thawing on ice, and then testing for loss of potency against a non-treated control in one of its recommended...

  13. mTOR Pathway

    Type
    Collection
    ...proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related GTP binding Raptor...

  15. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter pLL3.7-TNFa-shRNA3 72598 Expresses shRNA against mouse TNF-alpha mRNA from the mouse U6 promoter...

  16. Viral Production

    Type
    Collection
    ...possible, all plasmids used for viral production are propagated in the endA -mutated NEB Stable strain of E. ...

  17. Luciferase Plasmid Collection

    Type
    Collection
    ...CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...

  18. Plasmids for Stem Cell Research

    Type
    Collection
    ...human Sox2, KLF4, L-Myc, Lin28, OCT3/4, and shRNA against p53 in different gene/insert combinations A more...

  20. Cre-lox system

    Type
    Collection
    ...inducible; AAV donor vector AAV He 135659 pQcre1 Cre PpagA from B. anthracis Bacterial Tolonen 137051 pGL2399...
Showing: 21 - 40 of 393 results