We narrowed to 600 results for: ARL

...pH Red pH-sensitive fluorescent protein pHmScarlet . pHmScarlet is a pH-sensitive red fluorescent protein...the construct leads to a change in signal of a circularly permuted fluorescent protein (cpFP) or fluorescence...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression...Neuron. 2020 Jun 20. pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial...Calcium Red calcium sensor PinkyCaMP PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...

... a neurodevelopmental disorder that presents in early toddlerhood primarily affecting females at an incidence...criteria, defined as the presence of relatively normal early development, regression with a loss of spoken language... strong predictor of symptom severity. Broadly, early gene disruptions, missense mutations located in ...window) PMID: 6638958 Kankirawatana et al. 2006. Early progressive encephalopathy in boys and MECP2 mutations...

...activity, leading to the development of tumors in early adulthood, a disorder known as Li-Fraumeni syndrome...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...PMID: 12619118 . p53 mutant mice that display early ageing-associated phenotypes. Tyner SD, Venkatachalam...

...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks post-thaw, ...

...pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of...

...techniques are referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases. Browse...by cutting only one strand CRISPR C lustered R egularly I nterspaced S hort P alindromic R epeat, a bacterial... Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013)...

...language abilities. The disease can be classified as early-onset, where symptoms appear between a person’s ...appear during or after a person's mid-sixties. The early-onset form accounts for less than 10 percent of ...of all cases of Alzheimer's disease. Of these early-onset forms, a fraction of these are due to a mutation...

...Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The..., and a pair of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The...

... Genetic Edit References CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...from the mRNA due to alternative splicing, and an early frameshift mutation is more likely to result in ...experiments, testing multiple pegRNA designs is particularly important for prime editing. CRISPR Intereference...

...Companion to Methods in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector...J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation...

...Gi) - Inhibition mCherry Cre and Flp-dependent 8 Karl Deisseroth 177673 pAAV-nEF-Con/Foff DREADD Gi-mCherry...Gi-mCherry rM4D(Gi) - Inhibition mCherry Cre-dependent 8 Karl Deisseroth 135635 pAAV-S5E2-Gq-P2A-dTomato hM3D(...

...context for each TAL effector within an array, but early studies suggest the likelihood of context having... paper on TAL effectors in Nature Biotechnology early in 2011 and they described their kit for assembling...

....1WT-2A-mScarlet-KASH hSyn-driven, constitutive hSyn-driven expression of Kir2.1 and mScarlet-KASH for...

...Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN...Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used...

...: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences...26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human...

...bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel...mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage...PA28-gamma, PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2...

...an effort to speed up research of SARS-CoV-2 in early 2020, Ginkgo Bioworks (Link opens in a new window...

...donor DNA. Editing by CASTs is not considered "scarless", as the transposon homology sequences are integrated...

...derivatives of Coenzyme A (CoA). These tags are particularly well suited for labeling cell-surface proteins...

...to adult) and easy lab maintenance. Worms are particularly well suited for studies in cell biology, development...