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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 569 results for: CIT

Showing: 21 - 40 of 569 results

  1. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...many of which detail work to improve the tool’s specificity, orthogonality, and multiplexibility in various...endogenous loci with unprecedented speed and specificity. Figure 2: An overview of CRISPR and NHEJ/HDR...Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol . ...Engineered CRISPR-Cas9 nucleases with altered PAM specificities. Nature . 523(7561):481-5. PMID: 26098369 Komor...Rationally engineered Cas9 nucleases with improved specificity. Science . 351(6268):84-8. PMID: 26628643 Wang...

  2. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...generally contained at lower biosafety levels and elicit relatively low immunological effects in vivo . ...non-dividing cells with a low immune response and low toxicity. Although recombinant AAV does not integrate into...AAV is currently limited by its small packaging capacity (∼4.5 kb including ITRs), though there is a great...interest and effort directed toward expanding this capacity. Traditionally, AAV requires the presence of another...

  3. TALEN Guide

    Type
    Collection
    ... to recognize their targets with fairly high specificity. Fifteen years of research has shown that when...zinc fingers within an array can reduce their specificity. Two years ago, groups led by Jens Boch at the...though NN can also bind adenenine with lower specificity). With the template for studying this type of...likelihood of context having a negative effect on specificity is on par with ZF arrays, if not slightly lower...

  4. Viral Vectors

    Type
    Collection
    ...gene delivery in-vivo because of their mild immunogencity. These viruses can direct long-term transgene...genome. AAVs are limited by their small packaging capacity. Adenovirus Plasmids | Guide Adenoviruses are ...preferred for in-vivo studies because of its low immunogenicity. Different types of viruses also vary in the...linked above. Virus Expression Genome Packaging Capacity Virus Size (nm) Cells Infected Target Cell Genome...

  5. TALENs for Endogenous Zebrafish Genes

    Type
    Collection
    ...TTCTCAAAATGAGAGGGGtgaatctgaagttcaaCAAACCCAGCAGCACCAA CIT1 TAL3226 & TAL3227 TGAAGTTTAAATATGGAGgtcatggcagtgttaaAGATCTGTCCGCAGTCGA cited2 TAL3228 &...TGGTAGACCGCATGATGGcaatgaaccatggtcgTTTCCCAGATGCTGTGAA cited4a TAL3046 & TAL3047 TGAACCATGGTTCTGCTGgcggaggcctgcatggTTATCGCATGGGAATGAA cited4b TAL3048...

  6. Deisseroth INTRSECT Collection

    Type
    Collection
    ...developed, including additional fluorophores, excitatory and inhibitory opsins , genetically-encoded calcium...Flp AND NOT Cre Yes Dual recombinase-dependent: Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations...neuron glutamate cotransmission evokes a delayed excitation in lateral dorsal striatal cholinergic interneurons...Andrási T, Deisseroth K, Holmes A, Hájos N. 2019. Excitation of diverse classes of cholecystokinin interneurons...

  7. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC126648 CALCB calcitonin-related polypeptide beta CALC2, CGRP-II, CGRP2, FLJ30166 CALCR calcitonin receptor...MLT1 NFAT5 nuclear factor of activated T-cells 5, tonicity-responsive KIAA0827, NF-AT5, NFATL1, NFATZ, OREBP...component 5a receptor 1 C5A, C5AR, C5R1, CD88 CALCA calcitonin-related polypeptide alpha CALC1, CGRP, CGRP-I...receptor CRT, CTR, CTR1 CALCRL calcitonin receptor-like CGRPR, CRLR CAMP cathelicidin antimicrobial peptide ...somatostatin receptor 5 - ST2 suppression of tumorigenicity 2 - STC1 stanniocalcin 1 STC STC2 stanniocalcin...interleukin 9 receptor CD129 ST2 suppression of tumorigenicity 2 - TXLNA taxilin alpha DKFZp451J0118, IL14...polypeptide-related sequence B PERB11.2 NCR1 natural cytotoxicity triggering receptor 1 CD335, FLJ99094, LY94,...

  8. Plasmids for Stem Cell Research

    Type
    Collection
    ...are proliferative, unspecialized cells with the capacity to differentiate into many different cell types...every other cell type in the body and have the capacity to give rise to an entire organism. iPSC technology...which lower the risk of mutation and minimize tumorigenicity, an inherent disadvantage of iPS cell technology...Neurons Lentiviral Human Rapid Ngn2-induction of excitatory neurons from hiPSC-derived neural progenitor ...

  9. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ... Plasmids for In Vivo Imaging Far Red Protein Excitation/Emission Brightness Find Plasmids mCardinal 604...28.98 pEF.myc.ER-E2-Crimson Near-Infrared Protein Excitation/Emission Brightness Find Plasmids iRFP670 643...5.3 pPAS-E and pK-GAFm Photoactivatable Protein Excitation/Emission Brightness Find Plasmids PAiRFP1 690...

  10. Plan Your Experiment

    Type
    Collection
    ...High-fidelity Cas enzymes increase specificity Dual-nickase approach increases specificity but is less efficient Each...be able to completely avoid them. To increase specificity, consider using an engineered Cas enzyme with...use in the lab, but AAVs exhibits the lowest immunogenicity and are one of the least toxic methods for ...

  11. Neurodegeneration Research Collection

    Type
    Collection
    ...behavior and emotion, uncontrolled movements, memory deficits, as well as difficulty speaking and swallowing...cause of dementia. Symptoms include memory loss, deficits in decision-making and language abilities. The...Noteworthy: Study aberrant axon initial segment (AIS) plasticity with a motor neuron-specific reporter and a PAX7...

  12. Caltech Systemic Capsids

    Type
    Collection
    ...Category PI 37825 pAAV-CAG-GFP CAG GFP Control Boyden Citation Information When using the Caltech serotypes in...publications, please acknowledge Viviana Gradinaru and cite as follows: PHP.eB, PHP.S: Chan et al., 2017 Pubmed...L4 - AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For...

  13. Zhang Lab CRISPR Page

    Type
    Collection
    ...non-coding RNA elements capable of programming the specificity of the CRISPR-mediated nucleic acid cleavage....10.1126/science.1231143. PubMed . DNA targeting specificity of RNA-guided Cas9 nucleases. Hsu PD, Scott DA...RNA-guided CRISPR Cas9 for enhanced genome editing specificity. Ran FA, Hsu PD, Lin CY, Gootenberg JS, Konermann...

  14. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...in the 2009 Joung Nature Protocols paper . How to Cite this Kit These plasmids were created by your colleagues.... Please acknowledge the Principal Investigator, cite the article in which they were created, and include...

  15. Antibody Production

    Type
    Collection
    ...hybridoma-derived equivalent to ensure that potency and specificity have been retained. If the target antigen is ...pattern is compared to the mRNA expression pattern cited in the Tissue Atlas (Link opens in a new window)...

  16. Fluorescent Protein Guide: FRET

    Type
    Collection
    ... energy is non-radiatively transferred from an excited donor fluorophore to an acceptor. Since the transfer...transfer is measured using fluorescence microscopy by exciting the donor and measuring the emission of the acceptor...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ... VP16 transcriptional activation domain). For simplicity, other promoter elements are not shown. Tetracycline...and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours), and is relatively...

  18. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...Sciences has screened thousands of enhancers for specificity and brightness of fluorescent labeling across...collection includes the top vectors with the highest specificity and brightness, assessed using ssRNAseq or high-resolution...

  19. Water Bath Protocol

    Type
    Protocol
    ...bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside the water bath...

  20. Ligation Independent Cloning

    Type
    Protocol
    ...the first G on 3'-5' strand from cut site). For simplicity, only the 5' primer is shown here. Note: Use ...
Showing: 21 - 40 of 569 results