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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 667 results for: FIE

Showing: 21 - 40 of 667 results

  1. Brain Initiative Collection

    Type
    Collection
    ... the BRAIN Initiative below. Support has been identified by depositors and/or when BRAIN Initiative grants...high-quality and high-titer 100 µL solutions of purified viral particles that are suitable for in vivo ...our repository. AAV are distributed as 100 µL of purified viral particles in solution that are suitable ...

  2. Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids

    Type
    Collection
    ... Nat Biotechnol. 2013). The Joung lab has also modified their web-based ZiFiT Targeter software to enable...variants with no detectable off-target effects Modified PAM specifity of Staphylococcus aureus Cas9 (SaCas9...

  3. AAV Viral Preps

    Type
    Collection
    ... and ready to ship. All viral vector preps are purified by density centrifugation. Addgene’s comprehensive...AAV preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo ...

  4. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...high-titer preps are distributed as 100 µL solutions of purified viral particles that are suitable for in vivo ...virus-associated DNA, which is a sample of the purified DNA from the original plasmid that was used to...

  5. Resolute Plasmid Collection

    Type
    Collection
    ...transporters in the human genome with approximately 450 identified members. Many SLCs have mutations associated ...expects to accelerate the pace of research in the field of SLCs to the global benefit of basic academic ...

  6. AAV Molecular Tools

    Type
    Collection
    ...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-...transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP...

  8. Synthetic Biology - Assembly Standards Guide

    Type
    Collection
    ...Enzymes: SpeI , NotI , PstI Scar: ACTAGA Features: Modified RFC 10 with shortened prefix/suffix giving in-...Agel , Spel , Notl, Pstl Scar: ACCGGC Features: Modified RFC 10 w/ start and stop codons; Creates in-frame...

  9. AAVED

    Type
    Collection
    ...shared practical guide for all scientists in the field and every attendee of the meeting will be a co-author...Discussion) Technologies and Resources Missing in the Field Kenton Woodard 4:00 PM Wrap Up & Next Steps Melina...

  10. Microbiology Resources

    Type
    Collection
    ...contains plasmid tools for a range of microbial fields, including bacteria, viruses, parasites such as...measles) West Nile virus (WNV) Zika Don’t see your field listed? Know of some great plasmid tools for the...

  11. Worm Expression Resources

    Type
    Collection
    ...Cloning-free CRISPR for C. elegans , which uses purified Cas9, such as that expressed from pHO4d-Cas9 from...Efficient Genome Editing in Caenorhabditis elegans. A modified approach has also been developed by the Fire laboratory...

  12. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...opposite strand) within a small editing window specified by the gRNA. Adenine base editors convert adenine... fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter...

  13. Open Enzyme Collection

    Type
    Collection
    ...These enzymes are useful in both basic and applied fields as diverse as genomics, diagnostics, biodiversity...polymerase 165534 pOpen-T7gene5 T7 DNA Polymerase (unmodified) 165542 pOpen-KTHEAQpol KlenTaq1 polymerase 165543...

  15. TALEN Plasmids and Kits

    Type
    Collection
    ... Takashi Yamamoto This accessory pack contains modified pFUS array vectors, destination vectors and a ... Golden Gate TALEN and TAL Effector Kit. These modified pFUS vectors can reduce the number of module plasmids...

  16. Tetracycline Inducible Expression

    Type
    Collection
    ...expression. Several endogenous promoters had been identified that responded to stimuli, such as hormones or...On Elledge 19407 pTREtight2 Promoter contains a modified TRE that is silent in absence of binding tTA or...

  17. Rett Syndrome

    Type
    Collection
    ... known co-repressors or other transcriptional modifiers suggests the major function of MECP2 is to regulate...window) PMID: 1606614 1999 - Huda Zoghbi's lab identifies MECP2 as the genetic cause of RTT. (Link opens...

  18. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...introduce. We use Ultramer oligo from IDT (non-PAGE purified oligo is fine based on new data). For general ...homology arms. The Zhang lab typically use PAGE purified long oligos. For large changes (>100bp insertions...

  19. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...vector for bacterial expression; His,GST tag pE2c - Modified pENTR vector for C-terminal 3x HA tag fusion with...-GST for bacterial expression (Gateway) pE4n - Modified pENTR vector for plant expression of an N-terminal...

  20. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...flanking primers may be utilized. Choose a sequence-verified colony and inoculate into a maxi-prep culture....the bulk cells (see step 6 ). Select the clones identified with the desired deletion and move to larger ...
Showing: 21 - 40 of 667 results