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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 682 results for: RON

Showing: 21 - 40 of 682 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C ...TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4: hTLS-CHOP.pBSKS(+) FUS T7 ALS David Ron 21831 TLS 5:hTLS-CHOP....Friedreich ataxia Michael Ristow 15239 pWBC KIF5A T7 ALS Ron Vale 15402 pBlue-hFX(AA88)-HA FXN HA, HIS, AU1 T7...hTLS-CHOP.pCDNA1 FUS CMV ALS David Ron 22106 pWJPrP5 PRNP Dementia Susan Lindquist 22109 pWJPrP1 PRNP Dementia...Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia Ronald Kahn 11487...Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald Kahn 11505 pBabe...telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14666 pQE9-His-p97...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...23086203. PubMed Central PMCID: PMC3699407. Milo, Ron. "What is the total number of protein molecules per..., that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction...are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, ...ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for efficient proximity...of hydrogen peroxide. This radical reacts with electron-rich amino acids such as tyrosine on neighboring...Stephanie S., et al. "Directed evolution of APEX2 for electron microscopy and proximity labeling." Nature methods...peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology 30.11 (2012)...

  3. CRISPR Library Amplification

    Type
    Protocol
    ...expensive to ensure adequate quality, but this upfront cost will save headaches and expense later. At ...that are suitable for unstable or recombination-prone DNA. The use of electrocompetent cells is essential...commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library DNA (100 ng...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...A or T between position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats...must follow safety procedures and work in an environment (e.g. BL2+) suitable for handling HIV-derivative...responds differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin...

  5. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the...position. Sometimes an “N” is the result of an erroneous insertion by the sequencing reaction....

  6. Centrifugation

    Type
    Protocol
    ... to be centrifuged Water Background Information Front view of an open tabletop centrifuge with labels ... same components. The photo to the left shows a front view of an open tabletop microcentrifuge with the...

  7. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...chemicals that present a danger to people and the environment in biohazard containers. Biohazardous materials...protocols require bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2...

  8. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... 2 : Provides an optimal and stable chemical environment for the DNA polymerase to work adequately. Divalent...solvent and buffer capabilities provide a suitable environment for the DNA amplification reaction. Reference...

  9. Pouring LB Agar Plates

    Type
    Protocol
    ... strain grows, it's possible that: You used the wrong antibiotic. Your antibiotic is at too high a concentration... strain grows, it’s likely that you’ve used the wrong antibiotic, or confused your strains....

  11. Gibson Assembly Protocol

    Type
    Protocol
    ...and 30 bp annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins...

  14. Pipetting Protocol

    Type
    Protocol
    ...Background Information Anatomy of a Pipette This front view photo of a P1000 pipette shows the plunger ...

  17. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...redox. J Cell Biol. 2013 Apr 15;201(2):337-49. David Ron Redox/ROS Thiol redox-sensitive ratiometric sensor...Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine...electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron. 2012 Sep 6;75(5):779-...and cellular processes, or other factors like environmental conditions. With proper experimental design,... fluorescence properties can be affected by environmental factors and the sensor behavior may differ from...High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300...

  18. The Pleiades Promoter Project

    Type
    Collection
    ...CARTPT pEMS1492 intron-lacZ/NLS Ple22 CCKBR pEMS1493 intron-lacZ/NLS Ple23 CCKBR pEMS1494 intron-lacZ/NLS Ple24...CCKBR pEMS1495 intron-lacZ/NLS Ple25 CCKBR pEMS1496 intron-lacZ/NLS Ple26 CCL27 pEMS1497 intron-lacZ/NLS Ple27...CCL27 pEMS1498 intron-lacZ/NLS Ple28 CCL27 pEMS1499 intron-lacZ/NLS Ple29 CCL27 pEMS1500 intron-lacZ/NLS Ple30...CLDN5 pEMS1504 intron-lacZ/NLS Ple34 CLDN5 pEMS1505 intron-lacZ/NLS Ple35 CLDN5 pEMS1506 intron-lacZ/NLS Ple36...ICMT pEMS1593 intron-lacZ/NLS Ple123 ICMT pEMS1594 intron-lacZ/NLS Ple124 ICMT pEMS1595 intron-lacZ/NLS Ple125... pEMS1597 intron-lacZ/NLS Ple129 MKI67 pEMS1600 intron-lacZ/NLS Ple131 MKI67 pEMS1602 intron-lacZ/NLS ...pEMS1650 intron-lacZ/NLS Ple179 RLBP1L2 pEMS1651 intron-lacZ/NLS Ple180 RLBP1L2 pEMS1652 intron-lacZ/NLS...

  19. Brain Initiative Collection

    Type
    Collection
    .... AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core...recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...

  20. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-specific labeling. Neuron , 109 (9), 1449–1449. https://doi.org/10.1016/j.neuron.2021.03.011 PMID: 33789083...types and circuits. Neuron , 113 (10), 1507–1524. https://doi.org/10.1016/j.neuron.2025.04.035 PMID: 40403704...WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2-WPRE3...WPRE3-BGHpA AiP1691 AiE2366m SYFP2 Lamp5 interneurons Isocortex 220737 pAAV-AiE2366m-minBG-iCre(R297T)...R297T)-BGHpA AiP20068 AiE2366m Cre Lamp5 interneurons Isocortex 224015 pAAV-AiE0724m-minBG-SYFP2-WPRE3-BGHpA...BGHpA AiP12863 AiE0724m SYFP2 Lamp5 interneurons Isocortex 224051 pAAV-AiE0724m-minBG-iCre(R297T)-BGHpA...
Showing: 21 - 40 of 682 results