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Showing: 21 - 40 of 671 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C ...TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4: hTLS-CHOP.pBSKS(+) FUS T7 ALS David Ron 21831 TLS 5:hTLS-CHOP....Friedreich ataxia Michael Ristow 15239 pWBC KIF5A T7 ALS Ron Vale 15402 pBlue-hFX(AA88)-HA FXN HA, HIS, AU1 T7...hTLS-CHOP.pCDNA1 FUS CMV ALS David Ron 22106 pWJPrP5 PRNP Dementia Susan Lindquist 22109 pWJPrP1 PRNP Dementia...Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia Ronald Kahn 11487...Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald Kahn 11505 pBabe...telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14666 pQE9-His-p97...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...23086203. PubMed Central PMCID: PMC3699407. Milo, Ron. "What is the total number of protein molecules per..., that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction...are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, ...ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for efficient proximity...of hydrogen peroxide. This radical reacts with electron-rich amino acids such as tyrosine on neighboring...Stephanie S., et al. "Directed evolution of APEX2 for electron microscopy and proximity labeling." Nature methods...peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology 30.11 (2012)...

  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...°C. 0.1% Pluronic-F68 in PBS (A) Add 500 µL of 10% Pluronic-F68 to 49.5 mL PBS 0.01% Pluronic-F68 in PBS...Stocks of Pluronic F68 Pluronic F68, 10% solution A: 0.1% in PBS: 49.5 mL PBS + 500 µl Pluronic F68 B: 0.01%..., Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Pluronic-F68 NaCl MgCl 2 KCl Centrifugal filter units (...PBS (B) Add 5 mL of Buffer A to 45 mL PBS 0.001% Pluronic-F68 in PBS + 200 mM NaCl (C) (formulation buffer...Procedure Cover the filter membrane with 15 mL of 0.1% Pluronic F68 PBS and incubate for 10 min at RT Spin at ...and discard the flow through. Add 15 mL of 0.01% Pluronic F68 PBS and incubate for 10 min at RT. Spin at... discard the flow through. Add 15 mL of 0.001% Pluronic F68 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...Supermix for Probes no dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation...Reagent Preparation 1X PCR Buffer containing 0.05% Pluronic F-68: Prepare immediately before use and vortex...using. 500 µL of GeneAmp 10X Buffer 25 µL of 10% Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure...

  5. CRISPR Library Amplification

    Type
    Protocol
    ...expensive to ensure adequate quality, but this upfront cost will save headaches and expense later. At ...that are suitable for unstable or recombination-prone DNA. The use of electrocompetent cells is essential...commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library DNA (100 ng...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...A or T between position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats...must follow safety procedures and work in an environment (e.g. BL2+) suitable for handling HIV-derivative...responds differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin...

  7. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the...position. Sometimes an “N” is the result of an erroneous insertion by the sequencing reaction....

  8. Centrifugation

    Type
    Protocol
    ... to be centrifuged Water Background Information Front view of an open tabletop centrifuge with labels ... same components. The photo to the left shows a front view of an open tabletop microcentrifuge with the...

  9. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...chemicals that present a danger to people and the environment in biohazard containers. Biohazardous materials...protocols require bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2...

  10. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... Linear, Transfection Grade, VWR 75800-188 10% Pluronic F-18, Thermo Fisher 24040032 Glutagro, Corning...BCD TFX 1000 mL BalanCD HEK293 Media 10 mL 10% Pluronic-F68 40 mL 200 mM Glutagro Do not add selective...

  11. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... 2 : Provides an optimal and stable chemical environment for the DNA polymerase to work adequately. Divalent...solvent and buffer capabilities provide a suitable environment for the DNA amplification reaction. Reference...

  12. Pouring LB Agar Plates

    Type
    Protocol
    ... strain grows, it's possible that: You used the wrong antibiotic. Your antibiotic is at too high a concentration... strain grows, it’s likely that you’ve used the wrong antibiotic, or confused your strains....

  13. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...redox. J Cell Biol. 2013 Apr 15;201(2):337-49. David Ron Redox/ROS Thiol redox-sensitive ratiometric sensor...Dopamine Ultrafast neuronal imaging of dopamine dynamics (dLight1.1-1.5) Ultrafast neuronal imaging of dopamine...electrical events imaged in neurons with a fluorescent protein voltage probe. Neuron. 2012 Sep 6;75(5):779-...and cellular processes, or other factors like environmental conditions. With proper experimental design,... fluorescence properties can be affected by environmental factors and the sensor behavior may differ from...High-performance calcium sensors for imaging activity in neuronal populations and microcompartments. Nat Methods...Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 2013 Jul 18;499(7458):295-300...

  16. Gibson Assembly Protocol

    Type
    Protocol
    ...and 30 bp annealing to the target sequence. Avoid strong secondary structures in the homology region. Hairpins...

  17. The Pleiades Promoter Project

    Type
    Collection
    ...CARTPT pEMS1492 intron-lacZ/NLS Ple22 CCKBR pEMS1493 intron-lacZ/NLS Ple23 CCKBR pEMS1494 intron-lacZ/NLS Ple24...CCKBR pEMS1495 intron-lacZ/NLS Ple25 CCKBR pEMS1496 intron-lacZ/NLS Ple26 CCL27 pEMS1497 intron-lacZ/NLS Ple27...CCL27 pEMS1498 intron-lacZ/NLS Ple28 CCL27 pEMS1499 intron-lacZ/NLS Ple29 CCL27 pEMS1500 intron-lacZ/NLS Ple30...CLDN5 pEMS1504 intron-lacZ/NLS Ple34 CLDN5 pEMS1505 intron-lacZ/NLS Ple35 CLDN5 pEMS1506 intron-lacZ/NLS Ple36...ICMT pEMS1593 intron-lacZ/NLS Ple123 ICMT pEMS1594 intron-lacZ/NLS Ple124 ICMT pEMS1595 intron-lacZ/NLS Ple125... pEMS1597 intron-lacZ/NLS Ple129 MKI67 pEMS1600 intron-lacZ/NLS Ple131 MKI67 pEMS1602 intron-lacZ/NLS ...pEMS1650 intron-lacZ/NLS Ple179 RLBP1L2 pEMS1651 intron-lacZ/NLS Ple180 RLBP1L2 pEMS1652 intron-lacZ/NLS...

  18. Brain Initiative Collection

    Type
    Collection
    .... AAV vector for strong & rapid SYFP2 expression in forebrain GABAergic interneurons. The enhancer core...recombinase-dependent dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element...

  19. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...-specific labeling. Neuron. 2021 May 5;109(9):1449-1464.e13. doi: 10.1016/j.neuron.2021.03.011. PMID: ...WPRE3-BGHpA AiP11390 DLX2.0 SYFP2 Pan GABAergic interneurons Isocortex 222939 pAAV-DLX2.0-minBG-FlpO-WPRE-HGHpA...minBG-FlpO-WPRE-HGHpA AiP14532 DLX2.0 FlpO Pan GABAergic interneurons Isocortex 214518 pAAV-AiE2366m-minBG-SYFP2-WPRE3...WPRE3-BGHpA AiP1691 AiE2366m SYFP2 Lamp5 interneurons Isocortex 220737 pAAV-AiE2366m-minBG-iCre(R297T)...R297T)-BGHpA AiP20068 AiE2366m Cre Lamp5 interneurons Isocortex 224015 pAAV-AiE0724m-minBG-SYFP2-WPRE3-BGHpA...BGHpA AiP12863 AiE0724m SYFP2 Lamp5 interneurons Isocortex 224051 pAAV-AiE0724m-minBG-iCre(R297T)-BGHpA...BGHpA AiP14668 AiE0724m Cre Lamp5 interneurons Isocortex 214480 pAAV-AiE2103m-minBG-SYFP2-WPRE3-BGHpA AiP1287...

  20. Plasmids for Stem Cell Research

    Type
    Collection
    ...Single polycistronic lentiviral vector for the expression of human Oct4, Sox2, and Klf4 Polycistronic lentiviral...Minicircle Human Non-integrating mini-intronic plasmid (MIP) polycistronic expression of human Oct4, Klf4, ...functional midbrain dopaminergic neurons using a single polycistronic vector. PLoS One. 2011;6(12):e28719...Fibroblasts Neurons Lentiviral Mouse Direct conversion of fibroblasts to functional neurons by defined ...Dopaminergic Neurons Lentiviral Human Direct conversion of human fibroblasts to dopaminergic neurons. Proc Natl...Fibroblasts Neurons Lentiviral Human MicroRNA-mediated conversion of human fibroblasts to neurons. Nature....cholinergic neurons. Nat Commun. 2013;4:2183. Zhang Fetal Lung Fibroblasts Cholinergic Neurons Lentiviral...
Showing: 21 - 40 of 671 results