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We narrowed to 617 results for: TIL;

Showing: 21 - 40 of 617 results

  2. Viral Vector Guides and Plasmids

    Type
    Collection
    ... | Lentiviral Plasmids Lentiviral vectors are versatile tools because of their ability to transduce dividing...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 2006...

  4. Microbiology Resources

    Type
    Collection
    ...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...enhance expression of the message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden...

  7. CRISPR Plasmids - Tagging

    Type
    Collection
    ...easy to construct a donor vector, because MMEJ utilizes very short (around 20 bp) microhomologies as homology...

  8. CRISPR References and Information

    Type
    Collection
    ...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ...

  9. Rett Syndrome

    Type
    Collection
    ...Pediatr Neurol . 52, 585-591.e2. PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function...

  10. Zhang Lab CRISPR Page

    Type
    Collection
    ...Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors....

  13. Antibody Guide

    Type
    Collection
    ...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...

  15. Transfection for Recombinant Antibodies

    Type
    Protocol
    ...for the presence of particles still in solution. Continue to stir until all particles have dissolved. ... bottle and stir on a magnetic stir plate. Stir until all particles have dissolved. Note: The powder should...µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL BalanCD HEK293 Media 10 mL ...Glutagro Do not add selective reagents Store at 4 °C until use. We suggest preparing fresh solutions after ...HEK293 Feed 20 mL 200 mM Glutagro Store at 4 °C until use. We suggest preparing fresh solutions after ...Purification Protocol ). Store the supernatant at 4 °C until ready to use....

  16. AAV Production in HEK293 Cells

    Type
    Protocol
    ...water. While stirring, slowly add hydrochloric acid until the solution clears. Check the pH of the solution...adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This step is challenging...poured on them) and can be adjusted by carefully tilting the CS5 back and forth. Pro-Tip To help distribute...distribute the media amongst the five layers, tilt the CS5 such that the media goes toward the cap. If the ...Mix well between rounds of sonication. Sonicate until no live cells can be seen when stained with Trypan...

  17. CRISPR Library Amplification

    Type
    Protocol
    ...electrocompetent cells on ice for 15-20 minutes or until completely thawed. Chill a box of 200 µL micropipette...tube). Distribute evenly with a sterile spreader until all liquid has been absorbed by the agar. This usually...drips onto the lid. Let plates remain agar side up until dried before overnight incubation if needed. Place...addition of 10 mL cold LB and scrape for each plate until agar is clear (all bacteria have been removed). ...recovery media!). Do not proceed with Maxipreps or NGS until adequate transformation efficiency is obtained. ...

  18. Using a Light Microscope Protocol

    Type
    Protocol
    .... You can take entire courses on microscopy and still have more to learn, so, for this protocol, we'll...adjustment knob (the larger of the two focus knobs) until you can see the edges of the aperture are in focus...field of view and then slowly open the diaphragm until the edges are just out of view. Pro-Tip Looking ...arm to move the stage (and ultimately your slide) until your field of view is centered on a region of interest...

  19. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...buffers and do not use water). Microwave for 1-3 min until the agarose is completely dissolved (but do not ... OR let sit at room temperature for 20-30 mins, until it has completely solidified. Pro-Tip If you are...electrophoresis unit). Fill gel box with 1xTAE (or TBE) until the gel is covered. Pro-Tip Remember, if you added...additional wells of the gel. Run the gel at 80-150 V until the dye line is approximately 75-80% of the way ...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ... Work in a well-ventilated space and under a fume hood when working with the volatile reagents in the ...entire tube is dried but while the white pellet is still visible. Resuspend RNA pellet in RNase-free water...
Showing: 21 - 40 of 617 results