...Twinkle-APEX2-V5 TWNK CMV Spinocerebellar ataxia, infantile onset Alice Ting 131480 pORANGE GFP-CACNA1A KI...Halo_C_allele PLA2G6 Halo Dystonia-parkinsonism, Infantile neuroaxonal dystrophy Michael Ward 178167 RNF216...SUMO-Twinkle 43-372 TWNK His T7 Spinocerebellar ataxia, infantile onset Smita Patel 205055 SUMO-Twinkle 360-684 ...684 TWNK His T7 Spinocerebellar ataxia, infantile onset Smita Patel 205056 SUMO-Twinkle 43-684 TWNK His ...His Spinocerebellar ataxia, infantile onset Smita Patel 205706 pCMV-alphaS-2 SNCA CMV Parkinson's Randy ...

... ( Sander et al., Nat Biotechnol. 2011 ). REAL utilizes a set of 28 plasmids each encoding a single TALE...

... be labeled by co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery...

...substrates and the ACP and SFP Synthases. Users may still generate their own fluorescent or non-fluorescent...

...simple vector system to improve performance and utilisation of recombinant antibodies. BMC Biotechnol. 2006...

...Heterologous protein expression in E. coli, B. subtilis and P. pastoris - Gomis-Rüth Lab pOSIP plasmid...

...enhance expression of the message. Both vectors utilize homodimeric FokI domains. 38143 pC-GoldyTALEN Golden...

...easy to construct a donor vector, because MMEJ utilizes very short (around 20 bp) microhomologies as homology...

...activation). Genome support for bacteria ( E. coli, B. subtilis ), yeast ( S. cerevisiae ), worm ( C. elegans ...

...Pediatr Neurol . 52, 585-591.e2. PMID: 25801175 Tillotson and Bird. 2019. The Molecular Basis of MeCP2 Function...

...desired edit, as a large percentage of DSBs are still repaired by NHEJ. How do you determine that your...

...genome, transgene expression can be long-lived. The utility of AAV is currently limited by its small packaging...

...Return to top AAV - Cas9 mouse CRISPR-Cas9 is a versatile genome editing technology for studying the function...

...reprogramming, differentiation, and transdifferentiation utilize plasmids to increase expression of key factors....

...Discontinued Penn Vector Core Viral Vectors (Plasmids still available, other serotypes may be available) Based...

...options, such a 4-base pair codons, have also been utilized. A tRNA that recognizes this codon. An aminoacyl-tRNA...

...bind to a protein is powerful, but in order to utilize it in the lab, researchers must be able to detect...

... sensor, cytosolic or ER-targeted eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic...

...for the presence of particles still in solution. Continue to stir until all particles have dissolved. ... bottle and stir on a magnetic stir plate. Stir until all particles have dissolved. Note: The powder should...µm filter. Prepare aliquots and store at -20 °C until use. BCD TFX 1000 mL BalanCD HEK293 Media 10 mL ...Glutagro Do not add selective reagents Store at 4 °C until use. We suggest preparing fresh solutions after ...HEK293 Feed 20 mL 200 mM Glutagro Store at 4 °C until use. We suggest preparing fresh solutions after ...Purification Protocol ). Store the supernatant at 4 °C until ready to use....

...water. While stirring, slowly add hydrochloric acid until the solution clears. Check the pH of the solution...adjust to a final volume of 1000 mL. Stir at RT until fully dissolved. Pro-Tip This step is challenging...poured on them) and can be adjusted by carefully tilting the CS5 back and forth. Pro-Tip To help distribute...distribute the media amongst the five layers, tilt the CS5 such that the media goes toward the cap. If the ...Mix well between rounds of sonication. Sonicate until no live cells can be seen when stained with Trypan...