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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 581 results for: arl

Showing: 21 - 40 of 581 results

  1. Viral Production

    Type
    Collection
    ...immunogenic properties of the final product, particularly in large animal studies. Endotoxin contamination...detected. pAAV-Ef1a-mCherry-IRES-Cre was a gift from Karl Deisseroth (Addgene viral prep # 55632-AAVrg). ...being discarded and replaced with a new vial of early passage cells. Approximately 2 weeks post-thaw, ...

  2. Brain Initiative Collection

    Type
    Collection
    ...pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE Expresses optimized PdCO in frame with mScarlet under control of...

  3. CRISPR Guide

    Type
    Collection
    ...techniques are referred to as CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeat) technologies...AcrVA3.1, which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular...editors are based on cytosine base editors and similarly work by first converting cytidine to uridine. ...with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases Browse...by cutting only one strand CRISPR C lustered R egularly I nterspaced S hort P alindromic R epeat, a bacterial... Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013)...

  4. Zhang Lab CRISPR Page

    Type
    Collection
    ...Delivery AAV - Cas9 Mouse CRISPR ( C lustered R egularly I nterspaced S hort P alindromic R epeats) is ...oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold. The..., and a pair of annealed oligos can be cloned scarlessly into the vector before the sgRNA scaffold. The...

  5. Antibody Plasmid Collection

    Type
    Collection
    ...Companion to Methods in Enzymology 8, 94–103 (1995). Carlos Barbas Variant of the pComb3 phage display vector...J Immunol Methods. 2000 Aug 28;242(1-2):159-81. Carlos Barbas Vector system for expression, dimerisation...

  6. TALEN Guide

    Type
    Collection
    ...context for each TAL effector within an array, but early studies suggest the likelihood of context having... paper on TAL effectors in Nature Biotechnology early in 2011 and they described their kit for assembling...

  7. TALEN Plasmids and Kits

    Type
    Collection
    ...Gate TALEN 2.0 38142 RCIscript-GoldyTALEN Daniel Carlson The plasmids pC-GoldyTALEN and RCIsricpt-GoldyTALEN...Golden Gate TALEN 2.0 47388 pcDNA3.1-GoldenGate Charles Gersbach These new destination vectors can be used...

  8. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...: An Adaptive Immune System CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) sequences...26411297 Mali P, Yang L, Esvelt KM, Aach J, Guell M, DiCarlo JE, Norville JE, Church GM. 2013. RNA-guided human...

  9. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... YouTube . Abstract The prokaryotic clustered regularly interspaced short palindromic repeats (CRISPR)...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...

  10. Immunology Research Plasmids and Resources

    Type
    Collection
    ...bA350J20.7 RAET1G retinoic acid early transcript 1G ULBP5 RAET1L retinoic acid early transcript 1L - RELB v-rel...mutated in early stage tumors ARP, MANF, MGC142148, MGC142150 ARMETL1 arginine-rich, mutated in early stage...PA28-gamma, PA28G, REG-GAMMA RAET1E retinoic acid early transcript 1E LETAL, MGC125308, MGC125309, RAET1E2...

  14. Worm Expression Resources

    Type
    Collection
    ...to adult) and easy lab maintenance. Worms are particularly well suited for studies in cell biology, development...

  15. Plant Plasmids and Resources

    Type
    Collection
    ... in tomato, tobacco, M. truncatula , wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use ...

  16. Zebrafish Plasmid Collection

    Type
    Collection
    ... Resource Center (EZRC) - A repository at the Karlsruhe Institute of Technology (KIT) for the distribution...

  17. Open Enzyme Collection

    Type
    Collection
    ... lab costs and overcome supply chain issues, particularly in areas of the world where enzymes are harder...

  18. Retrograde AAV viral preps

    Type
    Collection
    ...Optogenetics Yizhar 125712 pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE CamKII Inhibitor Optogenetics Yizhar 154948...

  20. Bacterial Expression Systems

    Type
    Collection
    ...Burkholderia cenocepacia Miguel Valvano 47640 pCS-PesaRlux PesaR 3OC6HSL (Quorum sensing molecule) Escherichia...
Showing: 21 - 40 of 581 results