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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 492 results for: phy

Showing: 21 - 40 of 492 results

  5. AAV ddPCR Titration

    Type
    Protocol
    ... is genome copies and generally represents the physical number of viral particles. Users may need to run...

  7. Fluorescent Protein Guide

    Type
    Collection
    ...Biosensors Monitor small biomolecules or other physiological intracellular processes with genetically encoded...

  10. Chemogenetics Plasmids

    Type
    Collection
    ...have been engineered to respond to specific physiologically inert small molecules. There are four types...

  11. Optogenetics Plasmid Collection

    Type
    Collection
    ... light sensitive protein from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins...

  12. Antibody Production

    Type
    Collection
    ... columns. Formulation Buffer After affinity chromatography, antibodies undergo a buffer exchange using...

  13. Chemogenetics AAV Preps

    Type
    Collection
    ...Gq-P2A-dTomato hM3D(Gq) - Activation dTomato (physically separate) none 1, 9, PHPeB Dimidschstein No available...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...suitable for wholemount immunostaining. Biochem Biophys Res Commun. 2015 Jan 2;456(1):527-33. Gavin Wright...

  15. Promega Plasmid Collection

    Type
    Collection
    ...levels in lysates or live-cells, thus preserving physiological relevance. NanoBiT Assays NanoLuc Binary Technology...

  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...immunoprecipitation (enChIP) using CRISPR. Biochem Biophys Res Commun. 2013 Aug 11. pii: S0006-291X(13)01329...

  18. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    ... you? Physical titer: the concentration of viral particles containing viral genomes. Physical titers ...facilities report the physical titer of a viral solution (Figure 2). Because physical titers are used for...circled in red) report physical titer, which is measured by qPCR.   Physical titer is typically calculated...genome. Other than a theoretical maximum value, physical titers don’t necessarily give any indication of... AAV2 has been reported to have a near-perfect physical-to-infectious particle ratio of 1:1 (Zeltner et...for recombinant AAV2, the same study reported a physical-to-infectious particle ratio of 50:1 (Zeltner ... viral preparations is defined by the ratio of physical viral particles to infectious viral particles....

  19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...the truncated Phytochrome B (PhyBΔ) and the active protein binding domain of Phytochrome B interacting...splitting Cre and fusing each half with PhyB or PIF6, phytochrome-like fusion proteins from the plant Arabidopsis...respectively. The cofactor analog, phycocyanobilin (PCB), is required for PhyBΔ to be functional. Exposure to...systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria, has increasingly been...CyanoGate kit at Addgene. Vasudevan et al., Plant Physiol 2018. PubMed PMID: 30819783. Light up CreLite:...red light (650 nm) and its prosthetic group PCB, PhyB and PIF6 dimerize, bringing each terminus of Cre... to red light induces PhyBΔ and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer...
Showing: 21 - 40 of 492 results