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Showing: 21 - 40 of 783 results

  2. CRISPR Plasmids - gRNAs

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to ...

  3. CRISPR Plasmids - Cascade-Cas3

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3 is a unique member of the CRISPR family. After...

  7. CRISPR Plasmids - Tagging

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR system has been adapted to allow tagging...using them, are described below. Read our CRISPR 101 post for more information about HDR/repair template design...

  8. Luciferase Plasmid Collection

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    ...about DULIP in our 27 Hot Plasmids from 2016 blog post. LuTHy plasmids (Double‐Readout Lu minescence‐based...in our 28 Hot Plasmid Technologies from 2015 blog post. RNA Biology CMV-LUC2CP/intron/ARE, CMV-LUC2CP/ARE... STARR-seq in our 27 Hot Plasmids from 2016 blog post. pGL3-lRF , pGL3-LRR : Genomic tiles spanning a ...screening of transformants in cloning process. Lynne Postovit 119760 pSGDlucV3.0 Firefly/Renilla Insertion of...

  9. CRISPR History and Development for Genome Engineering

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    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution shows no signs of slowing ...researchers to further develop the CRISPR toolkit by posting lab protocols , providing tips from experts in ...check out Addgene’s CRISPR Guide and CRISPR blog posts , or find CRISPR plasmids for your research. Addgene...Scientists continue to wrestle with the possibility of postnatal and germline editing, with the International Summit...

  11. Transfection for Recombinant Antibodies

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    Protocol
    ...Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (96 h post-transfection): Add valproic... Feed. Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection): Add 4%...168 h post-transfection): Harvest antibody. Section 4: Harvest antibody At 168 hours (1 week) post-transfection...... Antibody Plasmid Collection Antibodies Blog Posts Molecular Biology Reference General Transfection...valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% BCD Feed...a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM ...

  12. SARS-CoV-2 Pseudotyped Virus

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    ...protocol for coronaviruses (184 KB) Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization...

  13. CRISPR Plasmids - Base Edit

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base editors — cytosine base editors...

  14. CRISPR Plasmids - C. elegans

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  15. CRISPR Plasmids - Drosophila

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  16. Retrovirus Plasmids

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    ...GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory element that increases...

  17. Antibody Production

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    ... Protocols Recipient Instructions Antibody Blog Posts Addgene Help Center...

  18. CRISPR Plasmids - Yeast

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    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR plasmids have been designed...

  19. Microbiology Resources

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    ...CRISPR Methods for Bacterial Genome Engineering blog post to see how Addgene depositors are making this possible...

  20. Worm Expression Resources

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    ...elegans . Return to top Other Resources Addgene Blog Posts An “elegans” Approach to Better CRISPR/Cas9 Editing...
Showing: 21 - 40 of 783 results