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...proteins. Return to top Other Resources Addgene Blog Posts Advancing Biology with Zebrafish: Genetic Tools ...

... to top Other Plant Resources Addgene Blog Blog Posts on Plant Biology RUBY-Red Siliques Tips for Arabidopsis...

... in targeted neuronal populations. Read our blog post about how to minimize leakiness in experiments using...

...Transfect cells. Friday (24 h post-transfection): Add 4% BCD Feed. Monday (96 h post-transfection): Add valproic... Feed. Tuesday (120 h post-transfection): Add 4% BCD Feed. Wednesday (144 h post-transfection): Add 4%...168 h post-transfection): Harvest antibody. Section 4: Harvest antibody At 168 hours (1 week) post-transfection...... Antibody Plasmid Collection Antibodies Blog Posts Antibody Guide General Transfection Protocol Recombinant...valproic acid supplementation During the 24–144 h post-transfection, supplement the flask with 4% BCD Feed...a total of 16% of the culture volume. At 72-96 h post-transfection, supplement the flask with 3.75 mM ...

...and plan your experiment, read our Luciferase blog post . Empty Backbones Browse or search for popular empty...screening of transformants in cloning process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter...

... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts Jump to: SpCas9 Plasmids GeCKO SAM AAV - In vivo...

...difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides an...

...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This... of dilutions of pHAGE-TO-dCas9-3XmCherry . 72 h post transduction, cells were assayed for mCherry expression...

...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...tips on AAV titering using ddPCR, read this blog post on ddPCR for AAV quantification. This protocol was... tips on AAV titering using ddPCR, read our blog post. Last reviewed on: August 31, 2023...

...may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This...production) Day 1: Transfect Cells Day 2 (am): 18 h post transfection - Remove media, replace with fresh ...

...(pm): Transfect packaging cells Day 2 (am): 18 h post-transfection. Remove media, replace with fresh media...cells. Virus can be harvested at 48, 72, and 96 h post transfection in individual harvests or a combined...

...the primers used to obtain each result above the posted sequence in the "View Sequence" link. Is your custom...

...for working with tissue culture in the lab. Blog post: 10 Basic tips for mammalian cell culture Aseptic...

...methods. Read more in our Restriction Cloning blog post . Figure 1: Restriction enzyme cloning of your gene...conditions. Read more in our Gateway Cloning blog post . Figure 2: Summary of Gateway cloning. Created ...enzyme cloning. Read more in our TOPO Cloning blog post . Figure 3: Summary of TOPO cloning. TOPO = topoisomerase...exonuclease. Read more in our Gibson Assembly blog post . Figure 4: Summary of Gibson assembly. Created ...project. Read more in our Golden Gate Cloning blog post . Figure 5: Summary of Golden Gate cloning. Created...secondary structures. Read more in our SLIC blog post . Figure 6: Summary of LIC. Created with BioRender.com...

...AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol...120 h. Optional: perform a media exchange at ~18 h post transfection. Changing the media can reduce toxicity...

...to the GCE bulletin board (GCEbb) listserve and posting your questions there. This peer-to-peer GCE community...

...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Western Blot Protocol Recombinant...

...in rodents and non-human primates. Read our blog post on systemic capsids for more information. Browse...better understand brain structure. Read our blog post on AAVrg for more information. Self-complementary...more about these and other initiatives in our blog post on using AAV for neuronal tracing . Browse AAV vectors...optimized for their use in rAAV vectors . Read our blog post on overcoming AAV size limitation for CRISPR delivery...development of further AAV applications. Read our blog post on split AAVs for more information. Viral Vector...packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

... and right homology arms. See Addgene's HDR blog post for more details. In some cases, there may be no... aureus Cas9. For more information, see our blog post on overcoming the AAV size limitation . Browse Addgene's...information, take a look at our collection of blog posts about CRISPR expression systems and delivery methods...expression? For more information, check out our blog post on validating CRISPR modifications . Cell Population...

...the goal is to identify those cells that survive post-selection. The selective pressure must be strong...our Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide...outline for using lentiviral gRNA libraries in postivie or negative screens. The use of appropriate controls...