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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 466 results for: Sele

Showing: 421 - 440 of 466 results

  1. Open Enzyme Collection

    Type
    Collection
    ...pET28a-LIC or other pET vectors are recommended). The selection marker for all plasmids in this collection is ...

  2. Pipetting Protocol

    Type
    Protocol
    ...Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle...experimental results. This protocol will guide you in selecting the correct pipette and pipette tip and using ...know the different parts of the pipette, how to select tips, and how to read a pipette, you’re ready to...display or the top of the plunger of the pipette. Select the pipette tip that is designed to fit your pipette...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ...bacteria that do not contain it by artificial selection (i.e. growing the bacteria in the presence of ...addition of an antibiotic to this gel allows for the selection of only those bacteria with resistance to that...stable, so it is potentially more effective at selecting only bacteria containing the plasmids of interest...the antibiotic at too low a concentration for selection. Negative Result 2: Neither Strains Grows If neither...

  4. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...most widely used method scientists employ to selectively move a specific piece of DNA from one plasmid...plasmid contains the expected size insert. By selecting the appropriate enzyme(s), one can either linearize...beginning your diagnostic digest, you will need to select a restriction enzyme or enzymes that cut your plasmid...

  5. Rett Syndrome

    Type
    Collection
    ... or the mammalian CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones...

  6. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...for a wide range of applications, with videos for select protocols in the right-hand column. You can find...and use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, how to dispense...pipette Watch the Video! Centrifugation Learn about selecting and using a centrifuge to separate different components...

  7. Retrograde AAV viral preps

    Type
    Collection
    ...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  8. Caltech Systemic Capsids

    Type
    Collection
    ...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  9. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...same technique can be used to move promoters, selectable markers, or any other DNA element between plasmids...restriction sites are present in a given sequence. When selecting restriction enzymes, you want to choose enzymes...information about restriction enzyme buffers). If you select enzymes that can function in the same buffer, it...

  10. Western Blot

    Type
    Protocol
    ...min for proteins >150 kDa. Select Start Run. When the run is complete, select Done. Section 5: Blocking...Gel Transfer Device. Close the lid of the device. Select the desired method and make sure the parameters...

  11. CRISPR Library Amplification

    Type
    Protocol
    ...high-throughput next generation sequencing (NGS). Select a restriction enzyme for digest that will cut the...propagation (origin of replication and antibiotic selection). This recombination, at a low rate, is not typically...Recombination is thought to be in part related to selective pressure. These recombined plasmids are much smaller...

  12. Luciferase Plasmid Collection

    Type
    Collection
    ... We also offer ready-to-use AAV preparations of select luciferase expression plasmids; these are noted...

  13. Biosensor AAV Preps

    Type
    Collection
    ...on Request service offers you even more options. Select from a vast catalog of eligible AAV plasmids and...

  14. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...proteins, point mutations, deletions, etc.) involves selecting one or more unique oligonucleotide primers that...There are a number of factors to consider when selecting sequencing primers, including: Is the primer unique...

  15. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...bacteria that do not contain it by artificial selection (i.e. growing the bacteria in the presence of ...flask. Using a sterile pipette tip or toothpick, select a single colony from your LB agar plate . Drop ...

  16. Ligation Independent Cloning

    Type
    Protocol
    ...the reaction mixture, and may provide counter-selection for negative clones as in the example shown here...removing the stuffer sequence and sacB counter-selection marker (see our restriction digest protocol )....

  17. Genetic Code Expansion

    Type
    Collection
    ... tRNA. Applications By making small changes in selected amino acids within a protein, any alterations ...

  18. Brain Initiative Collection

    Type
    Collection
    ...scientific research. Addgene's AAV are made from a selected set of plasmids that are currently in our repository...

  19. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...sequence to be amplified (usually 18-21bp). When selecting restriction sites, you should use a DNA analysis... longer the expected PCR product is. You should select an annealing temperature based on the melting temperature...

  20. Kit Free RNA Extraction

    Type
    Protocol
    ...for 1 hour. Lithium Chloride (Option B): LiCl selectively precipitates RNA versus DNA or proteins. Add ...
Showing: 421 - 440 of 466 results