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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 451 results for: abo.5

Showing: 441 - 451 of 451 results

  1. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...Recommended Concentration Ampicillin 100 µg/mL Bleocin 5 µg/mL Carbenicillin 100 µg/mL Chloramphenicol 25 µg...suggested amount, instead of the other dry ingredients above. Media without growth (top) and with growth (bottom...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...using two numbers separated by a slash (e.g. AAV2/5), where the first number indicates the serotype of...transduction efficiency and alter tropism. For example, AAV2/5, targets neurons that are not efficiently transduced...pieces of the transgene. The first vector contains the 5' portion of the gene and a splice donor sequence at...of the gene with a splice acceptor sequence at its 5' end. When these viral vectors co-infect a cell, they...much less efficient than with a single rAAV vector (∼5%). Gene fragments can also be reassembled via homologous...pseudotyped with viral capsids from serotypes 1, 2, and 5 display differential efficiency and cell tropism after... 317–334. https://doi.org/10.1007/s40259-017-0234-5 (Link opens in a new window) PMID: 28669112 (Link ...

  4. Adenovirus Guide

    Type
    Guide
    ...cells. Most rAdV vectors are based on adenovirus type 5 (Ad5) and have been produced by replacing the early...on our Adenovirus Plasmid Collection page . Figure 5: Overview of AdEasy™ adenoviral vector production ...), 3613. https://doi.org/10.1038/s41598-020-60238-5 (Link opens in a new window) PMID: 32107394 (Link ... of Sciences of the United States of America, 95 (5), 2509–2514. https://doi.org/10.1073/pnas.95.5.2509...adenoviruses using the AdEasy system . Nature Protocols, 2 (5), 1236–1247. https://doi.org/10.1038/nprot.2007.135...Journal of Genetic Syndromes & Gene Therapy, Suppl 5 , 001. https://doi.org/10.4172/2157-7412.s5-001 (Link...relative to transgene Purpose Transfer/Shuttle plasmid 5' ITR (LITR) in cis Left Inverted Terminal Repeat, ...

  5. Promoters

    Type
    Guide
    ...Promoters are about 100-1000 base pairs long and are adjacent and typically upstream (5’) of the sense...strand can initiate. The TATA box is a DNA sequence (5'-TATAAA-3') within the core promoter region where ...two short DNA sequences located at the -10 (10 bp 5' or upstream) and -35 positions from the transcription...Inducible by arabinose Promoter of the arabinose metabolic operon trp Repressible by tryptophan Promoter ...

  6. Chemogenetics Guide

    Type
    Guide
    ...activity. PSAM-5HT3 LGICs pair a PSAM domain with a 5-HT 3 serotonin receptor sodium- or potassium-selective...genetically engineered receptors. J Biol Chem. 266:5–8. PMID: 1670767 (Link opens in a new window) Tung...hardware-independent optogenetic inhibition. Sci Rep. 5:14366. PMID: 26399324 (Link opens in a new window)...to clinical translation of chemogenetics. Sci Adv. 5:eaaw1567. PMID: 31001591 (Link opens in a new window.... However, CNO is back-metabolized to clozapine and other clozapine metabolites and these can have off-target...concentrations of clozapine N ‐oxide (CNO). CNO is a metabolite of the antipsychotic clozapine and seems to be...experiments in this species may be entirely due to back-metabolism of CNO to clozapine, demonstrating the need for...

  7. Plan Your Experiment

    Type
    Guide
    ... regions, 5’ exons, or an exon known to code for an essential protein domain. Targeting a 5’ exon reduces...CRISPR/Cas9-based genome editing. Scientific Reports , 5 , 11315. https://doi.org/10.1038/srep11315 PMID: 26066060...exon coding for an essential protein domain could abolish protein activity and essentially function as a ...controls when working out a new protocol. Read more about how to design your gRNA . Synthesize and Clone Desired... other downstream methods. For more information about viral vectors and their production, see our viral...and Proteins In addition to the methods described above, you can also directly deliver mature mRNA or purified... approach. Similar to the mRNA method described above, this method is also transient, as the RNPs will...

  8. Optogenetics Guide

    Type
    Guide
    ...processes via protein engineering. Biochem Soc Trans. 41(5): 1183–1188. PMID 24059506 Klapoetke NC, Murata Y,...inhibition of neuronal activity in vivo. Sci Rep. 5:14807. PMID 26443033 Wietek J, Prigge M. 2016. Enhancing... to permit CIB1 binding. As shown in the figure above, this light induced binding can be utilized to control...dimerization. In addition to the applications described above, both LOV and Dronpa can control activity of a fused...

  9. Molecular Biology Reference

    Type
    Guide
    ...supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR ) xyl-5 λ– leu mtl-1 Top10 Invitrogen F- mcrA Delta(mrr-hsdRMS-mcrBC... Cloning Techniques Reference Guide Plasmids 101: 5 factors to help you choose the right cloning method...human genome with restriction enzymes would yield about two million DNA fragments, which is far too many...the molecular biologist’s toolbox. To learn more about different types of cloning methods check out our...restriction enzyme digestion. For more information about restriction enzymes check out NEB's website . Insert...endogenous expression levels). For more information about promoters, both bacterial and eukaryotic, as well...can be replicated easily and efficiently in a laboratory setting. Stable - Plasmids are stable long-term...

  10. Modular Cloning Guide

    Type
    Guide
    ... transcriptional unit (for example, a promoter, a 5' UTR, a signal peptide, a protein-coding gene, and...Learn about modular cloning (MoClo) and find MoClo kits deposited with Addgene, including the MoClo Toolkit...building synthetic genetic circuits, engineering metabolic pathways, assembling gene-editing tools, and more...editing, functional genomics, and more complex metabolic engineering and synthetic biology, compatible ... Techniques by Nicola Patron at The Sainsbury Laboratory Weber E, Engler C, Gruetzner R, Werner S, Marillonnet...

  11. Antibody Guide

    Type
    Guide
    ...but both rely on the same general strategy. Figure 5: Immunoprecipitation using bead-based antibody capture...are often used in clinical applications. Diabodies - Diabodies contain two Fab fragments, each recognizing...cells isolated from animals immunized as described above are used to create hybridomas, which produce large...different epitope, bound by short peptide linkers. Diabodies can be used for assembling protein nanostructures... Includes: Flow cytometry Read on to learn more about the applications common to each category, including...controls, and any special considerations to think about. For step-by-step instructions of many of these ...protein of interest, as described in the IP section above. Reverse the crosslink, isolate DNA, and perform...
Showing: 441 - 451 of 451 results