We narrowed to 500 results for: RAP
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TypeBlog Post... by binding the GyrA subunit of DNA gyrase and trapping it in the cleaved complex. This results in DNA...
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Antibodies 101: Reading a Flow Plot
TypeBlog Post...: While the plot generated often looks like the graph on the left, it might make more sense once you realize... -
Career Insights: Technical Support Specialist
TypeBlog Post...developments in the fields of gene and cellular therapies. Additional Resources on the Addgene Blog ... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog Post...in the notice of the new NRSA levels and in the graph below: The minimum should therefore rise from $43,692... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post... which could take 8 months or more. With such a rapid acceleration in the production of mouse lines that... -
Adeno-associated virus (AAV) Guide
TypeGuide...ideal for gene therapy, neuroscience research, and other biomedical fields. Gene Therapy One of the major...AAV) as novel vectors for human gene therapy . Current Gene Therapy, 3 (4), 281–304. https://doi.org/10.2174...serotypes: vector toolkit for human gene therapy . Molecular Therapy, 14 (3), 316–327. https://doi.org/10.1016...preferred choice to be used as viral vectors for gene therapy applications. This guide contains many viral vector-specific...applications of rAAV vectors is in the field of gene therapy to treat genetic disorders. rAAV vectors are favored...insertional mutagenesis. Several AAV-based gene therapies have been approved by the US Food and Drug Administration...dystrophy. Many clinical trials of AAV-based gene therapies are underway and are promising for the treatment... -
Lentiviral Vector Guide
TypeGuide...order to facilitate immunotherapy and gene therapy. Biomedicine & Pharmacotherapy , 128 , 110276. https...predict. In therapeutic applications, like chimeric antigen receptor T cell (CAR-T) therapy, the location...delivery of gene therapies. Lentiviral vectors are particularly effective for stem cell therapies, which rely...are common in clinical applications, like gene therapy, as you can more specifically dictate the target...oncogenes and other disease-associated genes. Gene Therapy Due to their relative safety and ability to transduce...transduce by other means. A concern with gene therapies is the possibility of transducing healthy tissues... of nonspecific transduction, lentiviral gene therapies have been shown to be relatively safe and effective... -
Antibodies 101: Normalization and Loading Controls for Western Blots
TypeBlog Post...Representative of three independent blots. B–C) Graphs showing the relative intensity of the β-actin (... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog Post... is incorporated. We were surprised to see how rapidly the efficiency of mutation incorporation falls ... -
March for Science
TypeBlog Post...freeze all grants, contracts The Guardian: Trump to scrap Nasa climate research in crackdown on “politicized... -
CRISPR 101: Homology Directed Repair
TypeBlog Post...external forces such as ionizing radiation, chemotherapeutics, and ultraviolet light. In this post, we will... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...PMC2853173. Sheridan, Douglas L., et al. "A new way to rapidly create functional, fluorescent fusion proteins:... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...be a big challenge. But ask any filmmaker or videographer and they’ll tell you, a lot of the fun is building... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...progression to resistance." Antimicrobial agents and chemotherapy 57.11 (2013): 5373-5383. Walkiewicz, Katarzyna... -
Adenovirus Guide
TypeGuide...platforms within the gene therapy landscape . Signal Transduction and Targeted Therapy, 6 (1), 53. https://...The immune system recognizes adenoviral elements rapidly and can mount a strong immune response quickly,...quickly, which has limited their use in therapeutic applications. However, this has also made them the most ...disadvantage for the use of rAdV vectors in gene therapy but has, however, proven to be very useful for ...adenoviral vectors are also being used in cancer therapy to stimulate anti-tumor immune response in different...implications and circumvention strategies . Current Gene Therapy, 11 (4), 307–320. https://doi.org/10.2174/156652311796150372...for outbreak pathogens . Human Vaccines & Immunotherapeutics, 13 (12), 3020–3032. https://doi.org/10.1080... -
Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility
TypeBlog Post...understanding how the body works, generating new therapeutics, and sometimes formulating new biological concepts... -
Plasmids 101: Dimers and Multimers
TypeBlog Post...which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies (e.g.... -
Antibodies 101: The Basics of Western Blotting
TypeBlog Post...sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein... -
Generating Mouse Models Using CRISPR/Cas9
TypeBlog Post... he is interested in using CRISPR to develop therapeutic applications that better human health. References... -
Plasmids 101: Using Transposons in the Lab
TypeBlog Post... like for iPSC reprogramming and gene and cell therapy, and have potential to overcome some of the limitations...