We narrowed to 500 results for: RAP

1. A Guide to Getting Started in Undergrad Research

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   Blog Post

   ...scientific process can help us understand how new therapies can be developed and applied. The current pandemic...

2. A History of Genome Engineering in Popular Culture

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   Blog Post

   ...what would happen if these corporations would extrapolate genetic engineering to exploit human beings. ...

3. RNA Interference in Plant Biology: New Tools for an Old Favorite

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   Blog Post

   ...with an Internal Marker for Silencing Allows for Rapid Identification of Loss of Function Phenotypes. Plant...

4. Gamma-Retroviral Vector Guide

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   Guide

   ...used in gene therapy applications to more specifically direct delivery of gene therapies. Common Uses ...gamma-retroviral vectors. Gene Therapy Gamma-retroviral vectors are a popular choice in gene therapy applications. There...There are many approved gamma-retroviral gene therapies to treat disease like cancer or immunodeficiency...Delivery of chimeric antigen receptor T cell (CAR-T) therapy is a particularly popular application of gamma-...and provide stable integration of the intended therapeutic. While similar to lentiviral vectors, they offer...centrifugation for CAR T cell manufacturing. Molecular Therapy — Methods & Clinical Development , 28 , 116–128...

5. Antibodies 101: Designing Your First Flow Panel

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   Blog Post

   ....e., happen most of the time). Figure 1 shows a graph from the BD® Spectrum Viewer, outlining a few commonly...

6. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

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   Blog Post

   ...crucial in the world of biological research, medical therapy, and even drug development (Grimm & Lavis, 2022...

7. With an Eye Towards the Future, We Look Back at the March for Science

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   Blog Post

   ...to convince me to spend a Saturday in the tourist-trap nightmare that is Times Square. But I made the sacrifice...

8. Special Delivery: Fluorophore Targeting for FRET Studies

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   Blog Post

   ...and purification is a snap using thin layer chromatography (Fessenden, 2009). However, these reagents ...

9. Selecting Your Plasmid Purification Kit

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   Blog Post

   ...check out our blogpost on lab automation here!) Rapid Isolation – If you’re in a hurry, several manufacturers...

10. CRISPR 101: Cytosine Transversion Editors

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    Blog Post

    ...? Base transversion editing is still advancing rapidly. If it follows a similar path to base transition...

11. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post

    ..., et al. “A versatile modular vector system for rapid combinatorial mammalian genetics.” Journal of Clinical...

12. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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    Blog Post

    ...PMC3656821. 10. Aasen, T., et al., Efficient and rapid generation of induced pluripotent stem cells from...

13. Modular Cloning Guide

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    Guide

    ...modular plasmids and enhanced MoClo protocols for rapid one-pot, multipart assembly, combinatorial design...library of constructs expressing GFP at 18 levels for rapid characterization in new organisms. Mobius Assembly...Mammalian Expression Stephan Gruber Platform for rapid assembly of expression vectors for multi-subunit... Drosophila John Doench 180-plasmid toolkit for rapid creation of CRISPR vectors compatible with various...

14. Chemogenetics Guide

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    Guide

    ... 4 ). Additional varenicline analogs, termed ultrapotent PSEMs (or uPSEMs), have also been developed as..., Basu J, Michaelides M, Sternson SM (2019). Ultrapotent chemogenetics for research and potential clinical...potent and selective chemogenetic actuator enables rapid neuronal and behavioral modulations in mice and ... engineer a receptor-effector complex for gene therapy. J Biol Chem. 276:1596-6. PMID: 11402033 (Link ...

15. Troubleshooting and Optimizing a Western Blot

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    Blog Post

    ..., for example, could indicate that you had air trapped between the membrane and gel during the transfer...

16. Cloning

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    Guide

    ...has been cloned into a donor plasmid, it can be rapidly shuttled into any compatible Gateway® Destination...Entry plasmids are also available, allowing for rapid cloning of PCR products into donor plasmids without...or a ligation step, making it an easy, cheap and rapid cloning method. So how does this work exactly? LIC...

17. CRISPR Guide

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    Guide

    ...comparative simplicity and adaptability, CRISPR rapidly became the most popular genome engineering approach...regulate known phenotypes, including resistances to chemotherapy drugs and toxins, cell viability, and tumor ...natural and engineered CjCas9 orthologs. Molecular Therapy , 31 (4), 1177–1187. PMID: 36733251 Harrington,...

18. Neurodegeneration Plasmid Collection

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    Collection

    ...178171 SGP11_Halo_N_allele SGP11 Halo ALS, Spastic paraplegia Michael Ward 178172 TBK1_Halo_C_allele TBK1 Halo...

19. Optogenetics Guide

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    Guide

    ... the LOV2 domain of Avena sativa phototropin 1 LOVETRAP reversibly sequester and release proteins from...

20. Plan Your Experiment

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    Guide

    ... frequently use AAVs to develop CRISPR-based therapeutics. One limitation of AAV vectors is that the packaging...