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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 545 results for: SEC

Showing: 521 - 540 of 545 results

  1. Typing CRISPR Systems

    Type
    Blog Post
    ...of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3...

  8. Optogenetics Guide

    Type
    Guide
    ...periods. These can range from msec (hChR2) to “long lasting,” e.g. seconds to minutes with stable step-...Zhang F, Bamberg E, Nagel G, Deisseroth K. 2005. Millisecond-timescale, genetically targeted optical control...

  9. CRISPR Library Amplification

    Type
    Protocol
    ...add 10 µL of the pool to 990 µL LB then perform a second and then third 1:100 dilution). Plate 100 µL of...not present on the most dilute plate, count the second plate (ie. the 1:10,000 dilution). After 12-18 ...

  11. CRISPR Guide

    Type
    Guide
    ...CRISPR in your own research. CRISPR Basics In this section, we will introduce the basic mechanisms of CRISPR...Generating a Knockout Using CRISPR Cas9 undergoes a second conformational change upon target binding. In this...have been developed, including editors based on a second ADAR variant that displays improved specificity...Oost, J. (2012). CRISPR immunity relies on the consecutive binding and degradation of negatively supercoiled...

  12. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...cell lines, such as HeLa and A549 cells, and even insect Sf9 cells , have also been developed. The newly...number indicates the serotype of the genome and the second number the serotype of the capsid. Pseudotyped ... splice donor sequence at its 3' end, while the second vector carries the 3' portion of the gene with ...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...telomeric DNA), which accumulates when T-loops (secondary telomeric structures) are deleted. Reduction in...

  15. Addgene's eBook Collection

    Type
    Guide
    ...101 July 2024 | Second Edition About Viral Vectors 101 July 2024 | Second Edition About This guide...

  16. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...digestion with EcoRI and AgeI. The oligos from section B contain the shRNA sequence flanked by sequences...polymerase has difficulty reading through the secondary structure of the hairpin sequence. Back to Top...

  17. Cloning

    Type
    Guide
    ...preserve the directionality of the cloning reaction. Second, the restriction site is encoded on both the insert... DNA that ends in sequences that form complex secondary structues. You can view an example of a LIC protocol...

  18. DNA Quantification

    Type
    Protocol
    ...despite the accuracy of the NanoDrop, if two consecutive samples have significantly different concentrations...
Showing: 521 - 540 of 545 results