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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 677 results for: RON

Showing: 21 - 30 of 677 results

  1. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...hTLS.pBSKS(+) FUS T7 ALS David Ron 21828 TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C ...TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4: hTLS-CHOP.pBSKS(+) FUS T7 ALS David Ron 21831 TLS 5:hTLS-CHOP....Friedreich ataxia Michael Ristow 15239 pWBC KIF5A T7 ALS Ron Vale 15402 pBlue-hFX(AA88)-HA FXN HA, HIS, AU1 T7...hTLS-CHOP.pCDNA1 FUS CMV ALS David Ron 22106 pWJPrP5 PRNP Dementia Susan Lindquist 22109 pWJPrP1 PRNP Dementia...Friedreich ataxia Ronald Kahn 11443 pBS human Frataxin (myc tag) FXN Myc T7 Friedreich ataxia Ronald Kahn 11487...Friedreich ataxia Ronald Kahn 11504 pBabe hygro human Frataxin FXN Friedreich ataxia Ronald Kahn 11505 pBabe...telangiectasia Didier Trono 14581 pSUPER.ATMi ATM H1 Ataxia telangiectasia Didier Trono 14666 pQE9-His-p97...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...23086203. PubMed Central PMCID: PMC3699407. Milo, Ron. "What is the total number of protein molecules per..., that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. This strong interaction...are approximately 2-4 million proteins per cubic micron in bacteria, yeast, and mammalian cells (Milo, ...ascorbate peroxidase (APEX), used as a genetic tag for electron microscopy, could be used for efficient proximity...of hydrogen peroxide. This radical reacts with electron-rich amino acids such as tyrosine on neighboring...Stephanie S., et al. "Directed evolution of APEX2 for electron microscopy and proximity labeling." Nature methods...peroxidase as a genetically encoded reporter for electron microscopy." Nature biotechnology 30.11 (2012)...

  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...°C. 0.1% Pluronic-F68 in PBS (A) Add 500 µL of 10% Pluronic-F68 to 49.5 mL PBS 0.01% Pluronic-F68 in PBS...Stocks of Pluronic F68 Pluronic F68, 10% solution A: 0.1% in PBS: 49.5 mL PBS + 500 µl Pluronic F68 B: 0.01%..., Hamilton 1X PBS pH 7.4 1X PBS-MK buffer 100X Pluronic-F68 NaCl MgCl 2 KCl Centrifugal filter units (...PBS (B) Add 5 mL of Buffer A to 45 mL PBS 0.001% Pluronic-F68 in PBS + 200 mM NaCl (C) (formulation buffer...Procedure Cover the filter membrane with 15 mL of 0.1% Pluronic F68 PBS and incubate for 10 min at RT Spin at ...and discard the flow through. Add 15 mL of 0.01% Pluronic F68 PBS and incubate for 10 min at RT. Spin at... discard the flow through. Add 15 mL of 0.001% Pluronic F68 + 200mM NaCl PBS. Spin at 3000 rpm for 5 min...

  4. AAV ddPCR Titration

    Type
    Protocol
    ...Supermix for Probes no dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation...Reagent Preparation 1X PCR Buffer containing 0.05% Pluronic F-68: Prepare immediately before use and vortex...using. 500 µL of GeneAmp 10X Buffer 25 µL of 10% Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure...

  5. CRISPR Library Amplification

    Type
    Protocol
    ...expensive to ensure adequate quality, but this upfront cost will save headaches and expense later. At ...that are suitable for unstable or recombination-prone DNA. The use of electrocompetent cells is essential...commercially prepared electrocompetent cells is strongly recommended. 800 ng pooled library DNA (100 ng...

  6. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...A or T between position 15-19. Avoid targeting introns. Avoid stretches of 4 or more nucleotide repeats...must follow safety procedures and work in an environment (e.g. BL2+) suitable for handling HIV-derivative...responds differently to puromycin selection. Addgene strongly recommends that you determine the optimal puromycin...

  7. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ... do not sequence the entire plasmid. Addgene strongly recommends that you sequence any portion of the...position. Sometimes an “N” is the result of an erroneous insertion by the sequencing reaction....

  8. Centrifugation

    Type
    Protocol
    ... to be centrifuged Water Background Information Front view of an open tabletop centrifuge with labels ... same components. The photo to the left shows a front view of an open tabletop microcentrifuge with the...

  9. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...chemicals that present a danger to people and the environment in biohazard containers. Biohazardous materials...protocols require bloodborne pathogens training. It is strongly recommended that anyone participating in BSL-2...

  10. Transfection for Recombinant Antibodies

    Type
    Protocol
    ... Linear, Transfection Grade, VWR 75800-188 10% Pluronic F-18, Thermo Fisher 24040032 Glutagro, Corning...BCD TFX 1000 mL BalanCD HEK293 Media 10 mL 10% Pluronic-F68 40 mL 200 mM Glutagro Do not add selective...
Showing: 21 - 30 of 677 results