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...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...This DNA is then used as a template to amplify a portion of the intended lentiviral insert. During this ...

... also lists several luciferase and fluorescent reporter plasmids that have been used for measuring viral...Plasmids ID Plasmid Description Industry PI Due to export control regulations, MERS and SARS-CoV materials...acids SARS-CoV-2 main protease (Mpro) activity reporters Reverse transcriptase, protease, and polymerase...

...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ...

... español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más ...

...plasmid webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters...MiniPromoters MiniPromoter Source Gene Construct Reporter Ple2 ADORA2A pEMS1142 EGFP/NLS Ple3 ADORA2A pEMS1143...CAG promoter pEMS1488 intron-lacZ/NLS References Portales-Casamar, E., Swanson, D. J., Liu, L., de Leeuw...

...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...mutating the amino acid residues of TetR that were important for tetracycline-dependent repression led to a...efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard Gu...

...Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and...

...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway...

...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster...

...Where can I find cloning resources or scientific support? Please see our Addgene Protocols for resources... may find helpful. Our Scientific and Customer Support Teams are also available to provide assistance ...

...Resources Addgene's Data Hub Curated technical reports from the research community demonstrating AAV performance...plasmid. Distribution of the original plasmid is important because it is the actual material being referenced...

...accessibility in research. For each antibody, we provide important details including the protein and plasmid sequence...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....

...Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether...PSAM is activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types...

...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes...

...every 35bp. Researchers are still determining the importance of context for each TAL effector within an array...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ...

...glutamatergic neurons, Cre payload. Must use with AAV8-DIO-Reporter viruses infected in postsynaptic cells. 5, 8 Don...express Cre, Flp payload. Must use with AAV8-fDIO-Reporter viruses infected in postsynaptic cells. 8 Don ...

... and adhesions between animal cells. They are important for holding cell structures together and reducing...translated at the endoplasmic reticulum, then are transported to the Golgi apparatus in vesicles to be packaged...

...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916)....

...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...

...choose and use antibodies, with an expert technical support team ready to answer your questions. All Antibodies...

... world. The NETRF is grateful for the generous support of philanthropic individuals and foundations, who...

...proteins as a first step for many different kinds of important experiments. In an effort to speed up research...

...pathways. Luciferase Plasmids Use bioluminescent reporters for studying gene expression, signaling pathways...

...applications where orientation of the insert is important. However, type I-F CASTs favor insertion in the...

...microbial opsins, which are membrane channels and pores derived from algae and archaebacteria, that can ...

...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL...

...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different...

... , a non-profit focused research organization supported by Convergent Research (Link opens in a new window...

... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids...

... Mouse IgG2a 206524 Anti-SNAT1 transporter [N104/37R] SNAT1 transporter Rat Mouse IgG2a 206525 Anti-Ankyrin-G... [N425/45R-2b] VAChT vesicular acetylcholine transporter Rat Mouse IgG2b 206673 Anti-VAChT [N6/38R-2b]...