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TypeBlog Post... español! Esta traducción fue regalada a Addgene por Daniel Mejías Sánchez, ¡y no podríamos estar más ...
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Viral Production
TypeCollection...expression and/or function. These data are sometimes reported or posted on the material page for the corresponding...recipient's initial thaw will be accounted for in our reported titers. Lentiviral vectors are titered using a...This DNA is then used as a template to amplify a portion of the intended lentiviral insert. During this ... -
CRISPR Plasmids - Empty gRNA Vectors
TypeCollection...Bullock and Port pCFD2-dU6:2gRNA 49409 Fly BbsI none S. pyogenes Virmilion Bullock and Port pDCC6 59985...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pAc-sgRNA-Cas9 49330 Drosophila BspQI yes, cut S...Drosophila BbsI none S. pyogenes Virmilion Bullock and Port pU6-BbsI-chiRNA 45946 Drosophila BbsI none S. pyogenes... -
TALEN Plasmids and Kits
TypeCollection...Guide CRISPR Plasmids Zinc Finger Kits TALENs are important new tools for genome engineering. Fusions of transcription... pFUS array vectors, destination vectors and a reporter vector for mammalian cell-based validation assay...N- and C-terminal domains (N153AA, C47AA). The reporter vector pGL4-SSA can be used for single-strand ... -
Lentivirus Plasmids
TypeCollection...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Parijs...Expresses shRNA under mouse U6 promoter; CMV-EGFP reporter cassette is included to monitor expression Kissler...transgene and CMV drives expression of RFP as a reporter. See plasmid 17618 for GFP plasmid. Cheng 17452... -
The Pleiades Promoter Project
TypeCollection...plasmid webpages. These plasmids are described in Portales-Casamar et al., 2010 . List of Pleiades MiniPromoters...MiniPromoters MiniPromoter Source Gene Construct Reporter Negative Control N/A pEMS1312 cre N/A pEMS1301 cre...intron-lacZ/NLS Additional Resources References Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks... -
Tetracycline Inducible Expression
TypeCollection...elements like the H1 RNA promoter. And, most importantly, the TetR protein has been modified to provide...identify the amino acid residues of TetR that were important for tetracycline-dependent repression. Mutating...efficacy by fluorescence, use Plasmid #35626 as a reporter for cDNA target. rtTA3 Ptet, miniCMV Howard Gu... -
Biosensor AAV Preps
TypeCollection...GRAB_OTmut (control) none Constitutive 1, 9 Li Voltage Reporter: Archon 108422 pAAV-CAG-FLEX-Archon1-KGC-EGFP-...Syn Archon1 EGFP Cre dependent 8 Boyden Voltage Reporter: Voltron 119036 pAAV-hsyn-flex-Voltron-ST Syn ...Voltron2-ST none Cre dependent 1 GENIE Voltage Reporter: JEDI-2P 179459 pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE ... -
Cancer Research Plasmids and Resources
TypeCollection...involved in cancer development. These pathways are important to normal cell signaling and physiology, and, ...location, their pathway dependencies are extremely important for classification and treatment. On each pathway... -
AAV Viral Preps
TypeCollection...on each item's material page. Actual titers are reported with each shipment. If you can't find the prep...Biosensors Calcium Sensors, Glutamate Sensors, Voltage Reporters Controls Fluorescent Proteins, Spagehetti Monster... -
CRISPR Plasmids - Parasites
TypeCollection...Trypanosoma (African sleeping sickness), Cryptosporidium (cryptosporidiosis), Leishmania (leishmaniasis), and... -
DNA Service - Cloning Grade DNA
TypeCollection...Where can I find cloning resources or scientific support? Please see our Addgene Protocols for resources... may find helpful. Our Scientific and Customer Support Teams are also available to provide assistance ... -
All Antibodies
TypeCollection...accessibility in research. For each antibody, we provide important details including the protein and plasmid sequence...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916).... -
Chemogenetics Plasmids
TypeCollection...Effector Molecules (PSEMs). PSAMs are coupled to an ion pore domain to create a ligand-gated ion channel. Whether...PSAM is activating or inhibitory depends on the ion pore domain it is coupled to. Addgene has two main types... -
Viral Prep Service for Plasmids and Pooled Libraries
TypeCollection...Resources Addgene's Data Hub Curated technical reports from the research community demonstrating AAV performance...plasmid. Distribution of the original plasmid is important because it is the actual material being referenced... -
Kazuhiro Oka Lentiviral Vectors
TypeCollection...set of lentiviral transfer vectors you can find reporter plasmids to test whether or not you've efficiently...to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases. Genes... -
TALEN Guide
TypeCollection...every 35bp. Researchers are still determining the importance of context for each TAL effector within an array...plasmid toolbox. Addgene has rapidly become an important resource for this new technology, and we hope ... -
Neuroscience
TypeCollection...our resources! Acknowledgments This project is supported by a NIH BRAIN Initiative grant (U24NS119916).... -
Synthetic Biology - Cloning and Genomic Tools
TypeCollection...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination... -
Antibodies
TypeCollection...choose and use antibodies, with an expert technical support team ready to answer your questions. All Antibodies... -
NETRF
TypeCollection... world. The NETRF is grateful for the generous support of philanthropic individuals and foundations, who... -
Ginkgo Bioworks COVID-19 Collection
TypeCollection...proteins as a first step for many different kinds of important experiments. In an effort to speed up research... -
Joung Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) Plasmids
TypeCollection...zebrafish Guide RNA expression plasmids for the EGFP Reporter Gene Guide RNA expression plasmids for endogenous... -
CRISPR Plasmids - CRISPR Transposases (CAST)
TypeCollection...applications where orientation of the insert is important. However, type I-F CASTs favor insertion in the... -
Optogenetics Plasmid Collection
TypeCollection...microbial opsins, which are membrane channels and pores derived from algae and archaebacteria, that can ... -
TALEN Engineering
TypeCollection...for Endogenous Human Genes TALENs for the EGFP Reporter Gene Additional Plasmids compatible with the REAL... -
Serotype Testing AAV
TypeCollection...Serotype Testing AAV These AAV encode fluorescent reporters and can be used to compare the tropism of different... -
Trimmer Lab NeuroMab Collection
TypeCollection... Mouse IgG2a 206524 Anti-SNAT1 transporter [N104/37R] SNAT1 transporter Rat Mouse IgG2a 206525 Anti-Ankyrin-G... [N425/45R-2b] VAChT vesicular acetylcholine transporter Rat Mouse IgG2b 206673 Anti-VAChT [N6/38R-2b]... -
Synthetic Biology - Overview
TypeCollection... modification, including shuttle, integration, reporter, and tagging vectors. Metabolism Browse plasmids... -
Adenovirus Plasmids
TypeCollection...plasmid, are recombined into a DNA molecule that incorporates sequences from both plasmids. This DNA molecule...