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  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...in rodents and non-human primates. Read our blog post on systemic capsids for more information. Browse...better understand brain structure. Read our blog post on AAVrg for more information. Self-complementary...more about these and other initiatives in our blog post on using AAV for neuronal tracing . Browse AAV vectors...optimized for their use in rAAV vectors . Read our blog post on overcoming AAV size limitation for CRISPR delivery...development of further AAV applications. Read our blog post on split AAVs for more information. Viral Vector...packaging. WPRE in cis Woodchuck hepatitis virus post‐transcriptional regulatory element; stimulates the...

  5. Western Blot

    Type
    Protocol
    ...like... Antibody Plasmid Collection Antibody Blog Posts Antibody Guide Immunocytochemistry Protocol Recombinant...

  6. Guide to Using Pooled Libraries

    Type
    Guide
    ...the goal is to identify those cells that survive post-selection. The selective pressure must be strong...our Viral Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide...

  7. Pouring LB Agar Plates

    Type
    Protocol
    ...Agar Plates You may also like... Plasmids 101 Blog Posts Mol Bio Protocols Cloning Protocols Introduction...

  10. Kit Free RNA Extraction

    Type
    Protocol
    ...For more tips on working with RNA, read this blog post on RNA extraction without a kit . Last Update: April...

  13. Educational Resources

    Type
    Guide
    ...Educational Resources From eBooks to videos and blog posts to protocols, our educational resources cover a ...

  14. Genetic Code Expansion

    Type
    Collection
    ...to the GCE bulletin board (GCEbb) listserve and posting your questions there. This peer-to-peer GCE community...

  15. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires that you have to express both a ..._LacZ 74293 Mammalian S. pyogenes Neo, mCherry Postovit pdCas9-DNMT3A-PuroR_v2 74407 Mammalian U6 yes,...

  16. Validated gRNA Sequences

    Type
    Collection
    ... CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA sequences that have been...

  17. CRISPR Library Amplification

    Type
    Protocol
    ...mL SOC per electroporation readily available for post-electroporation recovery of cells. Ensure access...

  18. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Addgene Viral Service Lentivirus Guide Virus Blog Posts Introduction This protocol describes how to use ...

  19. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates...) Read more Science Career Development posts ... of Research conference is organized by postdocs for postdocs. I am delighted to see enterprising early...changes to the way we view the graduate school and postdoc training years. There is no doubt that there are...appeared in eLife: "A Fair Deal for PhD Students and Postdocs" by Dr. Henry Bourne, Professor Emeritus at UCSF...know of many schools that have completely student/postdoc-driven biotechnology clubs such as Penn State, ...the grants and programs they need such as at the Postdoc Career Development Initiative (PCDI) in the Netherlands...

  20. Cre-lox system

    Type
    Collection
    ..., check out Addgene’s Plasmids 101: Cre-lox blog post. Based on these Cre-lox recombination principles...

  21. Molecular Biology Reference

    Type
    Guide
    ...and recommend checking out these two Addgene blog posts relating to common E. coli lab strains and E. coli...listed in the table below can be found in this blog post . Below you will find a few antibiotics commonly...

  22. Editor's Choice, July 2016

    Type
    Blog Post
    ...summarize the most popular post of the month and point out one or more additional posts that deserve a peek ...While I love all of our blog posts, my pick for the "editor's choice" post this month is from guest blogger...of the post also really helped put this technology into a concrete context. I recommend this post to anyone...research. All posts from July 2016 Click on the links below to read all of our excellent posts from July:...50,000 views over the entire blog and hosted new posts on topics ranging from new tools you can use to ...surrounding anything to do with CRISPR, our most popular post in July (with over 700 views) was an update on new...soon to be Addgene Scientist!) Mary Gearing, this post focuses on new data from the Kim and Joung Labs ...

  23. Editor's Choice, September 2016

    Type
    Blog Post
    ...discover what new post contributed the most to this record breaking month and to find other posts that deserve... I would love to see more posts like this on the Addgene blog. Read the post for the full details and ...and attention to detail when writing the post. The second post, “Running For Rare Disease, Running for ... Read All of Our Editor's Choice Blog Posts As I’m sitting in the San Francisco International Airport...listening to the Lion King soundtrack and writing this post, it is my pleasure to announce that we once again...look. 5 Great Apps for Lab Life This very useful post was contributed by guest blogger Sean Stacey from... help make your lab life a bit easier. Since the post doesn’t provide an exhaustive list of all the apps...

  24. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...several excellent guest posts from our depositors. Interested in writing your own post for the Addgene Blog...research, check out this post! R Bodies: Membrane-Rupturing Microscopic Tools In this post, Jessica Polka, formerly...of your target organism. In this post, Chris Richardson, a postdoctoral researcher in Jacob Corn’s lab,...gratitude on the blog by highlighting some of the great posts written by Addgene depositors. Below you’ll find...misleading artefacts in your experiments. Read this post from Erik Snapp, Associate Professor at Albert Einstein... Chemistry & Small Molecule Fluorophores In this post, Luke Lavis from Janelia Research Campus reminds...as those that occur during development. Read this post to learn more about recombinase-based state machines...

  25. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...Breakdown of Addgene's top blog posts from 2015 Our most popular posts continue to be those from our Plasmids...year for the Addgene blog. We’ve released over 80 posts on a diverse array of topics pertinent to the research...community, we’ve curated a list of our 10 top-viewed posts from 2015 and encourage you to give them a read ... Plasmids 101 and CRISPR 101 series. These posts aim to give researchers the background they need to get... incredibly happy to see that one of our career posts has made it to the top of the list this year. While... goals and believe the popularity of our career posts shows that we are doing just that. Do you like the... blog or to contribute ideas for your own guest posts by e-mailing us at [email protected]. Thank you again...

  26. Editor's Choice, August 2016

    Type
    Blog Post
    ...very interesting post about how we develop our in house lab management software. The post is written in ... Read All Our Editor's Choice Blog Posts The Addgene Blog has had quite the up-tick in views over the... Dewari who wrote this month’s most popular blog post on early user experiences with CRISPR alternative...detailed results of his survey can be found in the full post but TLDR: many users have had difficulty with NgAgo...editor’s choice picks this month, I’m picking two posts that describe methods to make single base edits ...generate many mutated plasmid constructs. The second post was contributed by Addgene Scientist Mary Gearing...cells are all discussed further in this informative post. Interestingly, this technology may make some uses...

  27. Deck the Lab 2022!

    Type
    Blog Post
    ...contestants!           View this post on Instagram                     A post shared by Xi Chen (@seleena78...2022          View this post on Instagram                     A post shared by 草方 (@gracie_fang)...of.           View this post on Instagram                     A post shared by Xi Chen (@seleena78...seleena78)   Here’s how to enter: Post your picture or video for either contest on Twitter or Instagram by...the hashtag #DeckTheLab, and tag @Addgene in the post! In January, Addgenies will vote on the entries...

  28. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    ...writing the first post so I signed up to write a second. While working on this post, I was offered and...blog post outlines 10 steps to take when starting to use Twitter as a scientist. While this post is designed...shared in the post. And the blog writing tips are elements I try to include in each post I write for the...professional advantage. It wasn’t until I wrote a blog post about a research paper that explored how scientists...started with the Addgene blog. I wrote my first blog post out of boredom. I had just started working in a ...When I saw a request for an Addgene guest blogger post about colony PCR, I signed up immediately. I had...Twitter, I found this paper. I wrote an Addgene blog post about it and shared the paper with my lab since ...

  29. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ...blog this year. Spoiler alert: many of the top 10 posts from this year were influenced by COVID-19 and our...new cloning grade DNA service. We've had 11 blog posts on COVID-19 featuring plasmid tools from depositors...further ado, here are the top ten! Top 10 blog posts from 2020 10. What's the Best Way to Elute and Store...about temperature? Find the details in this blog post. 9. Droplet Digital PCR for AAV Quantitation by...titers. Get the details on the methods in this blog post. 8. Chromoproteins: Colorful Proteins For Molecular... the year, Joanne Kamens followed up with a blog post with creative ways to continue career development...can't stably maintain them in the cell. This blog post features two mechanisms that cause plasmids to be...

  30. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...methods in this blog post. Hope you’ve enjoyed going through some of these top blog posts. We’d also like ...the most recent plasmid blog posts Find the most recent CRISPR blog posts Resources at Addgene Browse...page. So let’s get to it. Here’s the top 10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR...finding your clones in an experiment. Read this blog post for some positive selection (ex: antibiotic selection.... Learn more about the aptamer soup in this blog post. 5. Five Popular Model Organisms by Alyssa Cecchetelli...people are using for their research. In this blog post, we’ve covered five model organisms: mouse, fruit...
Showing: 61 - 90 of 783 results