...Microscopy-based lineage tracing can help illuminate how signaling pathways—such as those regulated by one-eyed pinhead—contribute... study also showed that in the absence of the signaling pathway regulated by one-eyed pinhead, incorrect...transform zygotes into adults and explain how signaling pathways control this process. Additional zebrafish...

...found that adding a triple nuclear localization signal or including fusions with Histone 2A or 2B can ...biotinylation via BirA and AviTAG. pMAK contains the signal peptide of MBP, followed by the desired nanobody...NB) sequence, an AviTAG, and 6xHis tag. The MBP signal peptide allows the NB to be exported to the periplasm...

... Fluorescent Protein Fusions Observing a bright signal for the first time after cloning an FP fusion with...illustrated the quantitative increase in fluorescent signal achieved when utilizing an optimized moxFP as opposed... opposed to standard FPs. The increase in signal is not due to a brighter FP, but rather because of the...

...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making it...single action potential-evoked axonal glutamate signals. Shading denotes SEM. D) Summary of ΔF/F0, Time-to-peak...Waldron Glypicans are important modulators of cell signaling pathways during embryonic development and cancer...

...you‘ll need, including sub-cellular localization signals, plant markers, and reporters (Engler et al., 2014...protein degradation on chimeric antigen receptor signaling (Kim et al., 2024). The EMMA Toolkit is another...et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor Laboratory,...

...the associated fluorochrome, emits a fluorescent signal that is captured by detectors. The strength of ... bind to a primary antibody thus amplifying the signal.       Intracellular protein targets, measured... FITC and that from PE. During compensation the signal arising from a specific fluorochrome is removed...

...provides a high contrast image in which a bright signal overlays a perfectly black background. In addition...to more rapid photobleaching and a reduction in signal intensity. 2-Photon microscopes restrict excitation...light-reducing pinhole. By collecting more photons, a higher signal to noise ratio can be achieved (6). Super-resolution...

...interacting domain (NID) a nuclear localization signal (not essential for localization) the C - t erminal...C-terminal Anti-MECP2 (D4F3) rabbit antibody from Cell Signalling Technology Additional Resources (Link opens in...

...vector that adds a C-terminal nuclear localization signal pGADCg - Gateway yeast expression vector that adds...adds an N-terminal myristoylation signal GST Protein purification pEBG - N-terminal GST ...

...cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A. 2012 May 29. Palecek...Chemical modulation of transcriptionally enriched signaling pathways to optimize the conversion of fibroblasts...

... do it (i.e. where the signals come from and what implications their signaling is likely to have). Anterograde...neuron to another at synapses resulting in faint signals (Schwab et al., 1979; Buttry & Goshgarian, 2015...

...improves protein solubility and stability. TRX and signal peptide tags help proteins with disulfide bonds...Plasmids designed for these systems may include a signal peptide (SP) that routes the protein through the...

...sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single pulse...pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten pulses...

...exactly what iRFPs achieved. Those proteins allowed a signal-to-background ratio in mammalian tissues 20-fold... In addition, PA FPs can improve the achievable signal-to-background ratio, thus, allowing higher resolution...

...material, cells or tissue, to produce a strong enough signal over background noise. This is not terribly problematic...large numbers of cells or when a high degree of signal to noise is desired. It also provides an excellent...

...molecular level. The brain is constantly influenced by signals it receives from the entire body, making it difficult...advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were very few...

...the riboswitch results in increased fluorescent signal. Prior to the development of these probes, fluorophore...less prone to photobleaching, and achieve better signal in vivo as compared to imaging with broccoli aptamers...

...amplify fluorescent signals in in vivo imaging: the 10xHA tag increased the fluorescent signal to noise ratio...

... Monoclonal antibodies can yield relatively low signal in immunoassays due to only having one binding ...you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal, with ...

...not need to be as high to achieve an observable signal in your experiment, therefore titer may not need...fluorescent proteins required to get a good fluorescent signal. It may not be necessary to concentrate this type...

...locations, measure fluorescent signals from the sensor, and send a signal including its position in the...

...displacement leads to a change in the fluorescent signal: untranslated RNAs appear yellow, and translated...reporter. Because it contains a nuclear localization signal, NLS-PCP-GFP then returns to the nucleus to bind...

... set, we would expect TagRFP to give a brighter signal than mCherry. In general, filter sets designed ...all organisms and that if you want the brightest signal, you may need to try several proteins in your system...

...classified function and a well established cellular signaling gene, thus providing a starting point for downstream...series of clones corresponding to known cellular signaling pathways as part of our Genetic Pathway Reference...

...technique can be applied to an important neuronal signalling pathway - if you’ve got a useful tool, there’...neuronal networks, and selectively activating signaling pathways. Many scientists came to the Addgene ...

...fluorescent reporter (iGluSnFR3) to study glutamate, a signaling molecule in the brain. “We hope that iGluSnFR3...these neurons using GCaMP7, measuring calcium signaling related fluorescence during morphine administration...

...antibody is ideal as it will help to amplify the signal. Some applications use either direct (primary-conjugated..., remember that a secondary should be used when signal amplification is needed.  Species  Next, check ...

...stable fluorescence signal, showing no or little deterioration or change of the signal during the course...

... an enzyme co-factor, a crosslinking agent, a signaling ion, and an allosteric modulator. Because of its...pdb-top069609. Palmer AE, Tsien RY. Measuring calcium signaling using genetically targetable fluorescent indicators...

...Gutless Ad maintain only the ITRs and the packaging signal (Ψ), both of which are essential for assembly of...sequences and retain only the ITRs and packaging signal in cis. Therefore, co-infection with a helper adenovirus...

...localizes primarily to the cell body with very little signal detected along the neurite (Figure 1.) Moreover...than other dark fluorescent proteins, it has good signal stability allowing for minimized basal FRET variability...

...conditions that give the strongest biotinylation signal, as measured via Western blot, and that result ... fusion protein and saturation of biotinylation signal. For Split-BioID, Schopp et al allowed 24 hours...

...decreasing the fluorescent signal from the first FP and increasing the signal from the second FP. Several...

...light to detect, measure, and control molecular signals, cells, and groups of cells in order to understand... into distinct populations based on fluorescent signal. Thus, FACS can be used to separate cells expressing...

...-expressing E. coli. The GV-specific ultrasound signal inside the tumors was overlaid with the anatomical... in situ expression. The GV-specific ultrasound signal is in hot and the anatomical B-mode images are ...

...Blot protocol and then blame the antibody when no signals are obtained. Or someone may heat an ELISA plate...during incubation steps in order to enhance the OD signals and then complain about high background. In many...

... was reading. SHH is involved in the hedgehog signalling pathway, which is a key regulator of all bilateral...development. Specifically, SHH is a morphogen, a chemical signal that diffuses down a concentration gradient exerting...

...SunTag scaffold, the intensity of the fluorescent signal is boosted and enables single molecule tracking... T cell receptor portion of the CAR facilitates signal transduction and release of cytotoxic granules ...

...minutes without any issues in toxicity. TurboID’s signal is significantly greater than miniTurbo at earlier...exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold compared to the standard...

...binding sites found on avidin are also ideal for signal amplification during these experiments. During ...interest leading to amplification of the reporter signal and easier detection. This strategy is referred...

...including the nervous system, development, cell signaling, aging, and mechanotransduction to name a few....Leifer’s lab utilized the worm to study calcium signaling in the entire adult brain, using a plasmid that...

...particle (the packaging signal Psi). Because the packaging plasmids lacked the psi signal, the genes encoding...

...conversion or the induction of intra- or intercellular signaling pathways (Ernst et al., 2014). Microbial rhodopsins...reached and the neuron fires, sending the electrical signal down the axon (Lodish et al., 2000). Most neurons...

...fluorescent signals produced by these bacteria can hinder correct result interpretation. Also, the signals from...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...Metabolism Networks & Gene Regulation Sensing & Signaling Strains Browse Addgene's collection of synthetic...

...have a FLAG epitope tag and nuclear localization signal at their amino-terminus. The expression vectors...

...conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection includes...