We narrowed to 125 results for: 1-Oct

...the dCas9-activator to your specific locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

... edits via homology-directed repair (HDR). Figure 1: Overview of Cas9 nickase cleavage and repair. Browse...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...collateral RNA degradation seen in bacteria. Figure 1: Overview of RNA targeting. Browse, sort, or search...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...with a genomic region of interest in vivo. Figure 1: Overview of purification of genomic loci using CRISPR...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...with target sites throughout the chromosome. Figure 1: Overview of visualizing genomic loci using CRISPR...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...occurs at a much lower efficiency than NHEJ. Figure 1: Overview of NHEJ and HDR following cleavage by Cas9...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...some mammalian systems have been developed. Figure 1: Overview of the basic CAST mechanism. Created with...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...Bacterial TAG Farren Isaacs 73545 pEvol-pAcFRS.1.t1 pAcFRS.1.t1 E. coli p-acetyl-l-phenylalanine (pAcF) Bacterial...Bacterial TAG Farren Isaacs 73547 pEvol-pAzFRS.1.t1 pAzFRS.1.t1 E. coli p-azido-l-phenylalanine (pAzF) Bacterial...recognized by the cognate orthogonal tRNA. Figure 1: Use of synthetase and target plasmids in genome code...7FTrp) Bacterial TAG Thomas Huber 207639 pEVOL-NBK-1 PylRS M. mazei Pyrrolysine Bacterial Cole DeForest...and tyrU gene replaced by GentR) and release factor 1. Expresses archaeal MjTyrRS/tRNA pair instead. For...122650 Mm-PylRS-AF/Pyl-tRNACUA PylRS M. mazei trans-cyclooct- 2-ene-lysine (TCOK) Mammalian TAG Howard Hang...

...activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort, or...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

... find a particular viral spike protein (MERS, CoV-1, CoV-2), modification, or variant. ID Plasmid Description...Collection Return to top Content last reviewed on 01 October 2025. Do you have suggestions for other plasmids...

...1774 for neomycin selection. Bob Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with...Biosafety Resource Guide Content last reviewed: 23 October 2025 Do you have suggestions for other plasmids...

...) element and the cis-acting EBNA-1 from Epstein-Barr virus. EBNA-1 binds oriP and allows for replication...Difficult to purify reprogramming proteins. Table 1: Key Features of Different Methods for Generating ...-based expression requires serial transfection of 1 or 2 plasmids that express the reprogramming factors...  Replicating oriP/Epstein-Barr nuclear antigen-1 (EBNA1) based episomes: These plasmids carry the origin...method Most iPSC research falls into two categories: 1) studies focused on better understanding the mechanisms...iPSCs experiences in the comments below! References  1. Sano, Masayuki, Minoru Iijima, Manami Ohtaka, and...Methods figures were adapted from this reference. 1. Malik, Nasir, and Mahendra S. Rao. "A Review of the...

... Figure 1 below) was free to anneal to complementary ssDNA molecules in vitro. Figure 1: Cas9 binding...allowed sequence replacement rates of approximately 1%. This is by no means a large number, but it was attained...who worked on this publication:         References 1. Richardson, Christopher D., et al. "Enhancing homology-directed...contributed by guest blogger Chris Richardson, a Postdoctoral Researcher in Jacob Corn’s lab. CRISPR-Cas9 ... optional donor DNA. Chris Richardson is a Postdoctoral Researcher in Jacob Corn’s lab investigating...

...your target gene! Tanenbaum et al., Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. Multi-bit genetic...light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2). In the dark, this peptide is caged by the...iLID nano and 800nM to 47µM for iLID micro; Figure 1). With this great affinity range, these LIDs have ...Figure 2). They are all available now at Addgene. 1 Lungu et al., Chem Biol. 2012 Apr 20;19(4):507-17....Guntas et al., Proc Natl Acad Sci USA 2015 Jan 6; 112(1):112-7. doi: 10.1073/pnas. CRISPR-Cas9 optogenetic... al., BMC Molecular Biology. 2013 August 20th; 14(1). TRICK: A method for visualizing the first round...for a variety of experimental purposes including (1) single or multiple, constitutive or inducible, gene...

...into more specialized cells with specific functions [1]. In many tissues, stem cells serve to replenish/replace...damaged cells that no longer function adequately [1]. Stem cells’ ability to differentiate into multiple...://www.linkedin.com/in/kmukherjeephd/. References 1. Dittrich, R., M.W. Beckmann, and W. Wurfel, Non-embryo-destructive...The potential of muscle stem cells. Dev Cell, 2001. 1(3): p. 333-42. PubMed PMID: 11702945. 6. Cowan, C....preimplantation genetic diagnosis. Hum Reprod, 2008. 23(1): p. 46-53. PubMed PMID: 17989069. 8. Takahashi, K... the ectopic expression of four embryonic genes- OCT4, SOX2, KLF4, and C-MYC. iPSCs were first generated...communication and publishing after completing her postdoctoral training from Massachusetts General Hospital...

...cellular proteins are modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation...three components required for translation (Figure 1): A tRNA that recognizes a specific ‘open’ codon ... open reading frame (ORF) of interest (Figure 2). 1. The phosphoserine OTS The backbone of the phosphoserine...modification allowed the deletion of release factor 1, the protein responsible for terminating translation...Rinehart lab: pCRT7 NT TOPO E17TAG GFP and pGEX-6P-1. The pCRT7 NT TOPO vector contains a mutant pSer-containing... can follow her on Twitter @nieminm.   References 1. Cohen, Philip. "The origins of protein phosphorylation...contributed by guest blogger Natalie Niemi, a postdoctoral fellow at the Morgridge Institute for Research...

...type of choice in vitro is known as reprogramming [1]. The process can be divided into two stages: Dedifferentiation...//www.linkedin.com/in/kmukherjeephd/.  References 1. Hochedlinger, K. and R. Jaenisch, Nuclear reprogramming...from human peripheral blood. Cell Stem Cell, 2010. 7(1): p. 15-9. PubMed PMID: 20621044. PubMed Central PMCID...induced pluripotent stem cells. Hepatology, 2010. 51(1): p. 297-305. PubMed PMID: 19998274. PubMed Central... pluripotent stem cells. Nat Biotechnol, 2014. 32(1): p. 84-91. PubMed PMID: 24291815. PubMed Central ...keratinocyte lineage. Methods Mol Biol, 2014. 1195: p. 1-12. PubMed PMID: 24510784. PubMed Central PMCID: PMC4096605...induced pluripotent stem cells. PLoS One, 2010. 5(1): p. e8763. PubMed PMID: 20098701. 31. Tang, Z.H.,...

...available as a substrate for another round. Figure 1: Overview of prime editing. Created with BioRender.com...CRISPR Blogs CasPEDIA Content last reviewed: 17 October 2025 Do you have suggestions for other plasmids...

...such as IL-8, IFNγ, and colony stimulating factor 1 (CSF-1). Additionally, tumor cells recruit regulatory...introduced the “Three Es of Immunoediting” nomenclature. 1. Eliminate - Latent tumors are recognized by effector...the tumor, activates hypoxia inducing factor (HIF-1), which promotes the production of VEGF to increase...This study used CRISPR gene editing to knockout PD-1 on immune cells harvested from patients and thereby...exploring new avenues in healthcare.   References 1. Vinay, Dass S., et al. "Immune evasion in cancer:...lymphoblastic leukaemia in children and young adults: a phase 1 dose-escalation trial." The Lancet 385.9967 (2015)...contributed by guest blogger Subhadra Jayaraman, a doctoral candidate at Binghamton University Cancer is one...

...acceptable. Aims should be mechanistic, doable by 1-3 people in 1-5 years, and contribute to resolving the overarching...possible, do at least two full length practices (~1 h) and get feedback. It’s also helpful to practice...contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...from Oregon Health Sciences University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz...and now serves as the Director of Student and Postdoctoral Programs at the Janelia Research Campus of the...