We narrowed to 933 results for: KIN

...CDK6 Cyclin-dependent kinase 4 or 6 CHK1 Checkpoint kinase 1 CHK2 Checkpoint kinase 2 Cop-1 Ring finger ...factor 1 ATM ATM serine/threonine kinase ATR ATR serine/threonine kinase B99 G-2 and S-phase expressed 1...PMAIP1 p14ARF Cyclin-dependent kinase inhibitor 2A p21 Cyclin-dependent kinase inhibitor 1A p48 Damage-specific...

...pegRNA + nicking sgRNA BbsI No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA...promoter, the type of gRNA (e.g., pegRNA, epegRNA, nicking sgRNA), cloning enzyme, selectable marker, and ...mRFP1 David Liu 140448 QPM-sgR (pTaU3) Plant TaU3 nicking sgRNA Eps3I + NcoI No Caixia Gao 141081 pYPQ141D-peg...Weber 172717 pnsgRNA Bacteria J23119 (BBa_J23119) nicking sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro...sgRNA-mCherry Mammalian, AAV hU6 + H1 pegRNA + nicking sgRNA No mCherry Hyongbum Kim 177180 pDAS12069_...

...cilia (Kogiso et al., 2020) Cytokines - SARS-CoV-2 infection induces a cytokine storm response in the body...spike protein constructs for making VSV pseudo virus. Addgene blog Scientists Map the...infectivity (Heurich et al., 2014). PCP4 plasmids - (Purkinje cell protein 4) is involved in beating of airway...body. For a full list of cytokines and related plasmids see our Immunology Resource Guide . Return to top...window) References Andersen, K. G., Rambaut, A., Lipkin, W. I., Holmes, E. C., & Garry, R. F. (2020). The...

...dynamic tracking of repetitive and non-repetitive genomic loci, chromosome painting, and RNA tracking in living... the most scalable genome editing technologies, making CRISPR perfect for genome-wide screens. This guide...very close proximity to the region to be edited, making the PAM sequence (NGG) limiting. This PAM is abundant...inhibit CRISPR by a variety of mechanisms, including blocking crRNA loading or PAM recognition, inhibiting Cas9...sites is sufficient to repress transcription by blocking initiation. More robust transcriptional repression...repression through cytosine methylation with improved kinetics LSD1 — repression through targeted removal of ...proteins that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA...

... of kinases of interest by mass spectrometry in a technique called serine-oriented library/kinase-library...NEDD4-2 (Pooled Libraries #111705-8) . Screening kinases The Mode #1 phosphosite library can also be synthesized...Lou, H. J., Turk, B. E., & Rinehart, J. (2018). Kinase substrate profiling using a proteome-wide Serine-Oriented...Rinehart, J., & Harper, J. W. (2015). The PINK1-PARKIN mitochondrial ubiquitylation pathway drives a program...Study Protein Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

...brighter fluorescence and faster kinetics (CaMPARI2) Improved methods for marking active neuron populations....fluorescent calcium sensors (fRCaMP1/2, fRGECO1/2) The kinetic mechanisms of fast-decay red-fluorescent genetically... novel analytical method for in vivo phosphate tracking. FEBS Lett. 2006 Oct 30. 580(25):5885-93. Wolf... Glutamate indicators with improved activation kinetics and localization for imaging synaptic transmission...Glutamate iGluSnFR glutamate sensor with improved trafficking to the plasma membrane Acute disruption of the...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy... red fluorescent protein to monitor exocytosis docking and fusion steps. Nat Commun. 2021 Mar 3;12(1):...

... Collections MJFF Parkinson's Plasmid Resource Michael J. Fox Foundation Parkinson's Disease Plasmid Resource...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation... researchers to assemble and share tools for Parkinson's disease research. This collection is part of ...

...Cre-dependent and constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice ...applications. Full references are below. The Cas9 knockin mouse can be purchased from Jackson Labs: Cre-dependent... for the mouse Rosa26 locus; Used to make Cas9 knockin mouse Vectors for cancer modeling 60224 : sgRNAs...10.1038/nbt.2647. Epub 2013 Jul 21. PubMed . Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing...plasmids AAV Plasmids with Cas9 Mouse CRISPR-Cas9 knockin mice for genome editing and cancer modeling. Platt...

...HaloTag, SmBiT, and LgBiT, useful for detecting and tracking key drug target proteins....analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc, HaloTag, ...signaling pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated...presence of a substrate. NanoBiT PPI assays enable kinetic, real-time analysis of protein interaction, association...

...Vector Guide Popular Lentiviral Transfer Plasmids Looking for lentiviral plasmids to express a CRISPR gRNA... ORF expression, multicistronic design enables tracking of viral transduction. See plasmid 46948 for additional...inducible knockdown, multicistronic design enables tracking of viral transduction. See Meerbrey et al. (2011... Reiser. Jakob Reiser 63586 psPAX2-D64V 2nd For making integrase-deficient lentivirus. David Rawlings ...

...important factor (for instance, looking for a different conjugate or looking for antibodies validated for...unused surface-binding sites in the wells with a blocking protein such as BSA, followed by several wash ... assay, called native ChIP, does not use cross-linking and instead relies on strong interactions between...the product information for similar antibodies, looking for ones that have identical features and epitopes...

... does not require a specific flanking sequence on the target RNA, making it a very flexible system. Other...rather than DNA, sometimes requiring a protospacer flanking sequence (PFS). In bacteria, Cas13 targeting also...):442-51. PMID: 23849981 Ishino Y, Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence...

... vector for "hit and run" reprogramming of adult skin fibroblasts to induced pluripotent stem cells. Stem...non-integrating episomes to allow sorting and dosage tracking of reprogramming factors Fluorescent tagged episomals...Stem Cell. 2012 Apr 6;10(4):465-72. Schöler Adult Skin Fibroblasts Cholinergic Neurons Lentiviral Human...Cell Stem Cell. 2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct...

...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

...Localization General Cytoskeleton Junctions Membrane Trafficking Additional Resources The subcellular localization... Protein Collection . Return to top Membrane Trafficking The secretory pathway is responsible for delivering...find markers for components of these membrane trafficking pathways and structures. ID Plasmid Structure...Fluorescent Protein Collection . Return to top Looking for more constructs or different fluorescent protein...

...RESOLUTE collection AAV Packaging from Addgene Parkinson's Research from The Michael J Fox Foundation Search...Screening Messer and MJFF Created for research in Parkinson's Disease; For target validation and drug design...Recommended Applications PI Don't See What You're Looking For? Please let us know what you would like to ...

...events and the systemic effects of Cre toxicity. Leakiness Leaky expression, where the gene of interest is...populations. Read our blog post about how to minimize leakiness in experiments using AAV vectors. Highlighted ...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid Description Gene/Insert...

...ncAA) in a protein of interest. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...and References GCE4All Research Center GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...

...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters, but are...a secreted version of the protein is available, making it ideal for a variety of applications. To learn...expressed from a T7 promoter for normalization. John Atkins 174050 pGWB-nLUC Firefly Creation of N-terminal...