... R, Heinz N, Hampf M, Bujard H, Gossen M. BMC Biotechnol . 2010 Nov 24;10:81. doi: 10.1186/1472-6750-10...-10-81. PubMed . Tet Systems: Science and Technology (Link opens in a new window) Do you have suggestions...

...neurons function. They are also delving into new technologies and resources in order to gain more fundamental...mitochondrial DNA using mitoBEs . Yi et al. Nat Biotechnol. 2023 May 22. See More AAV Viral Preps Find AAV...

...zebrafish ( Sander et al., Nat Biotechnol. 2011 ; Huang et al., Nat Biotechnol. 2011). The table below lists...

...assembled a list of FAQs about using the lab's CRISPR technology....assembled a list of FAQs about using the lab's CRISPR technology. Visit the Zhang Lab page and forum to learn ...

... Bakken TE, Barcelli T, Barta S, Bendrick J, Bertagnolli D, Bowlus J, Boyer G, Brouner K, Casian B, Casper...Fong O, Yao S, Mortrud M, Chong P, Loftus L, Bertagnolli D, Goldy J, Casper T, Dee N, Opitz-Araya X, Cetin...

...Human Mouse IgG2a 206584 Anti-Celf4/Brunol4 [N446/80R] Celf4/Brunol4 Human Mouse IgG2a 206585 Anti-Kv11.3...monoclonal antibodies for enhanced multiplex immunolabeling of brain. eLife . 8:e43322. PMID: 30667360 ...validated for use as intrabodies and nanoscale immunolabels in mammalian brain neurons. eLife . 8:e48750...

...Sterilize your lab bench by spraying it down with 70% ethanol and wiping it down with a paper towel. Maintain...

...appropriate materials. For example, you may use 70% ethanol, quaternary ammonium, or bleach solutions. Ask ...

... for all. Alternative fixation methods such as methanol or acetone may be better for some applications...

...working flame. Spray down the bench with a 70% ethanol solution and wipe down with a paper towel. Count...

...at a given region of the genome. Like nickase technology, this technique reduces unwanted off-target effects...

...wild-type FokI nuclease domains ( Sander et al., Nat Biotechnol. 2011 ): ID Plasmid C-terminal 0.5 TALE repeat...

...Ligation) assembly method ( Sander et al., Nat Biotechnol. 2011 ). REAL utilizes a set of 28 plasmids each...

...medium appears orange/yellow if using a standard phenol red pH indicator) because the buildup of waste ...

...dividing cells. CRISPR Activate and Interfere technologies also indirectly modify local chromatin state...

...activator system - Jaenisch Locus-specific ChIP technologies (iChIP & enChIP) using dCas9 - H. Fujii Disease...

... genes in zebrafish (Hwang and Fu et al., Nat Biotechnol. 2013). The Joung lab has also modified their...

...use with the Gateway® Cloning Platform (Life Technologies™) to permit construction of RAS pathway gene...

...opens in a new window) based on yeast display technology (Link opens in a new window) and an optimized...

...Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi Microbiology Plant Expression ...