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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 898 results for: NSI

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Showing: 41 - 60 of 898 results

  1. Antibody Production

    Type
    Collection
    ...and stained with Coomassie blue. Protein band intensities are measured using ImageJ software and a standard...standard curve. Using ImageJ software, the band intensities of both the antibody and impurities are measured...anti-GFP antibody), the target antigen is first transiently expressed in mammalian cells. To learn which ...

  2. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...3XGFP Microtubules ensconsin GFP William Bement 26742 EMTB-mCherry Microtubules Ensconsin mCherry William...Return to top Cell Junctions Cell junctions are responsible for forming connections and adhesions between...Membrane Trafficking The secretory pathway is responsible for delivering proteins to the extracellular ...

  3. Antibody Plasmid Collection

    Type
    Collection
    ...yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize the ... NeuroMab collection — monoclonal antibodies extensively validated for neuroscience research applications...Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells...

  4. mTOR Pathway

    Type
    Collection
    ...production through glycolysis to fuel these energy-intensive processes. mTORC1 also blocks catabolic processes...substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related...known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion of MTOR...

  5. Viral Production

    Type
    Collection
    ...staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed. The...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

  6. Plant Plasmids and Resources

    Type
    Collection
    ... through de novo induction of meristems . A comprehensive multi-purpose toolkit that enables targeted,...for Arabidopsis Transformation Save Time with Transient Plant Leaf Transformations Engineering the Plant...interdisciplinary exchange, open technologies, and responsible innovation for improvement of sustainable agriculture...

  7. Neurodegeneration Research Collection

    Type
    Collection
    ...speaking and swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within the huntingtin...or way to prevent progression of PD. Treatment consists mainly of managing symptoms through medication...organelles. (Mattia et al. EMBO Rep. 2018) Study intrinsically disordered protein regions with FUS and HNRNPA1...

  8. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ... ). The phosphorylation sites (“phosphosites”) consist of a central phosphoserine site flanked by 15 amino...Additional Resources See our dedicated Genetic Code Expansion collection page Read about Using Phosphoserine...Phosphorylation Read about GCE4All: Making Genetic Code Expansion Accessible...

  9. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...empty backbones or reporters . Experimental Considerations Recombinase Recognition Sites Each enzyme functions...in-stock viral preps feature Cre-dependent tools, or consider our AAV Packaged on Request service for more options...in-stock viral preps feature Flp-dependent tools, or consider our AAV Packaged on Request service for more options...

  10. Deisseroth INTRSECT Collection

    Type
    Collection
    ...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...recombinases, viruses, and important controls to consider as part of experimental design. How-to guide (...Arber S, Lüthi A. 2016. Midbrain circuits for defensive behaviour. Nature 534(7606):206-12. PubMed (Link...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ...codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci...861-72. Yamanaka Plasmid Human Non-integrating transient expression of human L-Myc, Lin28, Sox2, Klf4, ...35. Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion...

  12. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...conditions. pKa is the pH at which fluorescence intensity drops to half its maximum value. Maturation is...-LSSmOrange - Bacterial Expression mKeima (pH sensitive) 440 620 3.5 6.5 3 h Monomer pFA6a-link-yomKeima-CaURA3...to Red) Protein Excitation (nm) Emission (nm) Transition (nm) Brightness pKa Maturation Structure Plasmids...

  13. AAV Viral Preps

    Type
    Collection
    ...vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control...

  14. CRISPR Plasmids - CRISPR Transposases (CAST)

    Type
    Collection
    ... integrate donor DNA. Editing by CASTs is not considered "scarless", as the transposon homology sequences... visiting our CRISPR plasmids page . Find a comprehensive list of CRISPR resources by visiting our CRISPR...

  15. Optogenetics Plasmid Collection

    Type
    Collection
    ... a light sensitive protein from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins...

  16. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs ...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually...

  17. Microbiology Resources

    Type
    Collection
    ...plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio experiment...system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection is organized...

  18. Worm Expression Resources

    Type
    Collection
    ...transparent body of the C. elegans hermaphrodite consists of 959 somatic cells, which makes them attractive...elegans and related nematodes. WormBook - A comprehensive, open-access collection of original, peer-reviewed...

  19. TALEN Guide

    Type
    Collection
    ...available as a kit from Addgene , custom arrays consisting of 12-31 repeats can be assembled and inserted... Zinc Finger Consortium with Dr. Voytas, has considerable experience in the DNA targeting field. A third...

  20. Open Enzyme Collection

    Type
    Collection
    ...Open Enzyme Collection consists of more than 75 genes for enzymes that are considered "workhorses" of molecular...
Showing: 41 - 60 of 898 results