...yeast, plants, or insect cells. Benefits include consistency between lots and the ability to optimize the ... NeuroMab collection - monoclonal antibodies extensively validated for neuroscience research applications...Recombinant mAb Collection . Once the plasmids are transiently transfected into mammalian tissue culture cells...

...production through glycolysis to fuel these energy-intensive processes. mTORC1 also blocks catabolic processes...substrate 1 (proline rich) PTEN Phosphatase and tensin homolog Rag RRAGA RRAGB RRAGC RRAGD Ras-related...known as PRR5; proline rich 5 PTEN Phosphatase and tensin homolog Rictor RPTOR independent companion of MTOR...

...transcription factors for Agrobacterium-mediated transient expression or plant transformation - Silin Zhong...CRISPR-mediated Plant Base Editors Save Time with Transient Plant Leaf Transformations RNA Interference in...interdisciplinary exchange, open technologies, and responsible innovation for improvement of sustainable agriculture...

...high transduction efficiency and direct high but transient transgene expression. γ-Retrovirus Plasmids | ...8 Kb 80-130 Dividing/Non-dividing Yes Low AAV Transient or Stable* single stranded linear DNA ∼4.5 Kb ...Dividing/Non-dividing No* Very Low Adenovirus Transient double stranded linear DNA >8 Kb 105 Dividing/...

...those regions. Locus-specific ChIP technologies consist of insertional chromatin immunoprecipitation (iChIP...molecules such as TAL proteins and the CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9...Identification of proteins associated with an IFNgamma-responsive promoter by a retroviral expression system for...

...staining and the molecular weight and relative intensity of the viral capsid proteins are analyzed. The...preparation shows that the vast majority of the vectors consist of full particles (white arrowhead) relative to...targeting integrated copies of the lentiviral Rev responsive element (RRE) gene and the cellular ribonuclease...

...transgene expression. There are some safety considerations to evaluate when working with lentivirus: these... genome, it has the potential for insertional mutagensis. The 2nd and 3rd generation lentiviral systems...1a-driven GFP and shRNA under the control of a tet-responsive H1 promoter Trono 11651 pLVUT-tTR-KRAB 3rd Inducible...

...speaking and swallowing. HD is caused by abnormal expansion of a repeating CAG triplet series within the huntingtin...or way to prevent progression of PD. Treatment consists mainly of managing symptoms through medication...Use a FRET-based method to monitor structural transitions in protein self-organization. Wan et al. Cell...

...entries describing enzyme activity, experimental considerations, and more. CasPEDIA is a community resource...visualization of CRISPR-Cas9 outcomes, as well as comprehensive evaluation of their effect on the coding sequence...DNA Design Tool (EuPaGDT) gRNA design tool with extensive selection of eukaryotic pathogen genomes (200+...

...recognition sequences (e.g. Lox sites, FRT sites) inside of the introns (A,D). The addition of these recombinase...recombinases, viruses, and important controls to consider as part of experimental design. How-to guide (...Arber S, Lüthi A. 2016. Midbrain circuits for defensive behaviour. Nature 534(7606):206-12. PubMed (Link...

...virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing multiple...44,580 KSHV Tiling Library 180272 Knockout KSHV Glaunsinger 3rd NA 24,000 BLaER1 pgRNA Library 183825 Knockout... found to match all requested criteria. Please consider using fewer filtering options. Browse all libraries...

...Reporter Constructs Luciferase is the enzyme responsible for the bioluminescence found in a diverse number... the emission of photons. The robust signal, sensitivity, wide dynamic range, and resistance to photobleaching...depending on the application. Firefly luciferase is considerably brighter than Renilla luciferase, but is more...

...codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci...861-72. Yamanaka Plasmid Human Non-integrating transient expression of human L-Myc, Lin28, Sox2, Klf4, ...35. Baldwin Fibroblasts iTSCs Lentiviral Mouse Extensive Nuclear Reprogramming Underlies Lineage Conversion...

...3XGFP Microtubules ensconsin GFP William Bement 26742 EMTB-mCherry Microtubules ensconsin mCherry William...Return to top Cell Junctions Cell junctions are responsible for forming connections and adhesions between...

...Collection Browse the Michael Davidson comprehensive collection consisting of ORFs tagged with a variety of ...

...vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality control...

... a light sensitive protein from cryptochromes, phytochromes, or light-oxygen-voltage-sensing (LOV) proteins...

...was originally used. Here are a few questions to consider when choosing a gRNA: Does a given gRNA sequence...enzyme activity, PAM requirements, experimental considerations, and more, created and maintained by the Doudna...

...1000000013 ) Depositing Labs: Keith Joung This kit consists of plasmids and strains used to engineer ZFNs ...Oligomerized Pool ENgineering (OPEN) method. This kit consists of 5 individually packaged plasmids and 3 individually...

...plasmids, from networks and gene regulation to sensing and signaling, to perform your synbio experiment...system. Fluorescent Protein Guide : Addgene's extensive fluorescent protein plasmid collection is organized...