We narrowed to 735 results for: RAN-1

..., or the beginning of the coding sequence. Figure 1: Overview of CRISPR interference. Browse, sort, or...CRISPR plasmids for transcriptional repression of target genes. CRISPR...Catalytically dead Cas9 (dCas9), or dCas9 fused to a transcriptional repressor peptide like KRAB, can knock down...down gene expression by interfering with transcription. This interference is most efficient when the gRNA...

...for any application TMN200 (200 mM sodium chloride, 1 mM magnesium chloride, 20 mM Tris at pH 8.0) Suitable...adeno-associated viral vector. Hum Gene Ther Methods . Feb;25(1):72-82. PMID: 24191859 Ye, et al. 2015. Safety and...enhanced transduction of the retina following subretinal administration and enhanced transduction and spread...cite Boye, et al. 2016. Impact of Heparan Sulfate Binding on Transduction of Retina by Recombinant Adeno-... from the University of Florida for enhanced transduction of the eye and other tissues. As part of our...Y444F) serotype is demonstrated to have enhanced transduction of the retina following intravitreal injection... AAV2(Y444F) serotype also displays enhanced transduction of the trabecular meshwork following intracameral...

...Expression mEGFP 488 507 34 6 Monomer (A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression...TurboRFP-pBAD - Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV:tdTomato - Mammalian Expression...-pBAD - Bacterial Expression mRFP1 584 607 13 4.5 1 h Monomer pcDNA3-mRFP - Mammalian Expression pMXs-...Mammalian Gateway Destination Vector pENTR4-HaloTag (w876-1) - Gateway Entry Vector pCS2+/C-Halo , pCS2+/N-Halo...pSNAPf-C1 - Mammalian Expression pENTR4-SNAPf (w878-1) - Gateway Entry Vector pCS2+/C-SNAPf , pCS2+/N-SNAPf... Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+8CmOrange - Zebrafish/...urchin pCS2+8NmOrange - Zebrafish/Xenopus/Worm/Sea urchin mOrange-N1 - Mammalian Expression mOrange-C1 - Mammalian...

...enzyme and guide RNA) for your experiment (Figure 1). You will decide how to express Cas9, the delivery...finally how to validate your genetic edit. Figure 1: Flow chart describing the general framework of a ...population. Some cells may remain wild type due to either (1) a lack of gRNA and/or Cas9 expression or (2) a lack...Biology , 1239 , 197–217. https://doi.org/10.1007/978-1-4939-1862-1_10 PMID: 25408407 Dixit, A., Parnas, O...fused to transcriptional activators that need to be within a given range of the transcription start site... interfere with transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa... perform by chemical transfection or electroporation. Expression can be transient, or you can generate...

...Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in a new window) . TF...Browse the Zhang lab’s comprehensive human transcription factor collection, available at Addgene. The...The collection can be used for screening transcription factors for cellular programming or cancer drug ...Library The MORF library is a comprehensive human transcription factor collection that contains 1,836 genes ...collection, can be purchased at Addgene. Each transcription factor isoform has a unique 24 bp barcode that...that simplifies identification of the transcription factor isoform in pooled screens. This collection ...collection and library can be used for screening transcription factors for applications such as cellular programming...

...the dCas9-activator to your specific locus. Figure 1: Overview of CRISPR activation. Created with BioRender.com...dead dCas9 fused to a transcriptional activator peptide can increase transcription of a specific gene. ...

...in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette for expression of both a “+” and “-”...different cell types. Zinc finger arrays can be transferred in-frame to these vectors. Plasmid...either OPEN or modular assembly can be easily transferred in-frame to any of these vectors using unique...

...research and innovation programme and EFPIA. Figure 1: Representation of RESOLUTE mission. The combination...CRISPR knockout library targeting human transmembrane transporters. Feedback Please send your feedback to...carrier proteins (SLCs) are the largest family of transporters in the human genome with approximately 450 identified... Medicines Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives...allow a fast and efficient sub-cloning into a wide range of Gateway-compatible pDEST vectors. ID Plasmid ...overexpression library of human SLC proteins. Human Transporter Knockout Libraries 213695 221409 221410 Superti-Furga...

...Biology, 2(9), 537–541. DOI: 10.1021/sb400021j Figure 1: Multiplexing CRISPR knockdown to modulate expression...controlling the number of mismatches we can obtain a range of expression levels. This collection comprises ...Together, these plasmids allow to explore the whole range of expression levels for these two reporters simultaneously... S. (2018). Tuning dCas9’s ability to block transcription enables robust, noiseless knockdown of bacterial...

...gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids 159654... and 159655 are single guide expressing double stranded break constructs. Plasmids 171098 and 175570 allow...

...activation-induced cytidine deaminase (AID). Figure 1: Overview of CRISPR base editing. Browse, sort, or...generate single base pair edits without double stranded breaks. Cytosine base editors are created by fusing...creating a C to T change (or a G to A on the opposite strand). Likewise, adenine base editors have been engineered...

...) a T ranscriptional R epressor D omain (TRD) the TRD contains a Nuclear receptor Co-Repressor 1/Silencing...affecting females at an incidence of approximately 1:10,000. It is characterized by apparently normal development...reactivation, RNA editing, gene replacement therapy, RNA trans-splicing, and protein replacement to reverse the...cases Rett syndrome can be inherited. In females, random X inactivation results in approximately half of...disease severity. The MECP2 protein is a global transcriptional regulator of thousands of genes and studies...studies have suggested roles in transcriptional repression, activity dependent de-repression, chromatin remodeling...DNA and recruit known co-repressors or other transcriptional modifiers suggests the major function of MECP2...

...fluorescent proteins in budding yeast. Sci Rep, 9 (1):2234. https://doi.org/10.1038/s41598-019-38913-z ...von Wirén, N., Frommer, W. B. (2007). A cytosolic trans-activation domain essential for ammonium uptake....

...for brain circuit mapping and connectomics. Figure 1: Reconstruction of a sparse set of neurons across ...opens in a new window) , in collaboration with The Francis Crick Institute (Link opens in a new window) , ..., H.G.J., Leible, D., Leung, K.S., Magno, C.A., Meeran, A., Michalska, J.M., Rieger, F., Wang, C., Wu,...

...1774 for neomycin selection. Bob Weinberg 10676 pMKO.1 GFP MoMLV U6-driven plasmid for shRNA expression with...into multiple plasmids for safety: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked... the transgene fused to eGFP. Hans Clevers 18760 MSCV IRES Luciferase MSCV Plasmid for transgene expression...and transfer plasmids. Viral... Viral Plasmids Gamma-Retroviral Plasmids Gamma-Retroviral Plasmids Transfer Plasmids...encoded on RNA. The genome includes a reverse transcriptase and an integrase to insert the viral genome ...can take advantage of this to stably integrate transgenes of interest into a variety of cell types. Wild-type...

...and interrogate with conventional reagents. Figure 1: Axon guidance cues and receptors regulate the development...researchers with powerful tools to investigate neurotransmission, synapse formation, neural connectivity, plasticity...pairs bridge the presynaptic and postsynaptic membranes at neuronal synapses. Image credit: IPI Browse... top Integrins Integrins are a family of mechanotransduction receptors, comprised of alpha-beta subunit...

... Caltech and can help guide your decision. Figure 1: Decision tree for choosing your AAV capsid. Your ...tdTomato Control Boyden 83900 pAAV-mDlx-GFP-Fishell-1 Dlx GFP Control Fishell 99130 pAAV-mDlx-NLS-mRuby2....AX with GRE or transgenic animal Oligodendrocytes - AAV-PHP.eB with GRE or transgenic animal Brain vascular....AX with GRE or transgenic animal L5 & Inhibitory - AAV-PHP.eB with GRE or transgenic animal L2/3 & L4...AAV.CAP-B10 with GRE or transgenic animal Excitatory - AAV.PHP.N with GRE or transgenic animal For PNS applications... pAAV-S5E2-GFP-fGFP E2 regulatory element GFP, membrane-targeted GFP Control Dimidschstein 192552 pAAV-CAG... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...packaging transfection. In my lab we regularly use the ratios below: 1 Transfer Plasmid : 1 pHelper: 1 RepCap... plan to transfect: 1 to 10 Fill in experimental details on the spreadsheet: Name your viral prep...calculator in the comments section below. References 1. Choi, Vivian W., et al. "Production of Recombinant...prevent future headaches. To help you plan PEI transfections for AAV packaging, considering using this PEI...Calculator. AAV packaging typically requires transfecting three plasmids at specific molar ratios. To ...PEI calculator for AAV packaging Use the PEI transfection calculator for AAV packaging Select the plasmid...RepCap OR 3 Transfer Plasmid : 5 pHelper: 2 RepCap Select what type of tissue culture dish you will be ...

...integration. Introduction to Viral Vectors Figure 1: Wild-type virus propagation and use of viral plasmids...packaging capacity, high transduction efficiency, and high but transient transgene expression.They can induce...130 nm Dividing Yes Moderate AAV Transient or Stable* Single-stranded linear DNA ∼4.5 kb 18–26 nm Dividing.../Non-dividing No* Very Low Adenoviral Transient Double-stranded linear DNA 5–36 kb 105 nm Dividing/Non-dividing...of viral vectors and find viral production and transfer plasmids for a wide array of research purposes...are versatile tools because of their ability to transduce dividing and non-dividing cells. These virusal...immunogencity. These viral vectors can direct long-term transgene expression, but generally don't integrate into...

...formulation buffer is phosphate-buffered saline containing 1 mM sodium azide. This buffer is not compatible for...extracted from cells, separated by SDS-PAGE, transferred to a membrane, and labeled with the antibody and a species-specific...expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the heavy and light...anti-GFP antibody), the target antigen is first transiently expressed in mammalian cells. To learn which ... The fluorescence pattern is compared to an untransfected control. Immunohistochemistry (IHC) Tissue sections...antibody. Protein expression is compared to an untransfected control and the protein size is confirmed. Residual...contain low levels of co-purified plasmid from transfection. This residual plasmid is extracted and amplified...