We narrowed to 556 results for: SEC

...projecting to the starter cell). Once in the secondarily infected cells that lack the G protein, the virus...

...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...

...-surface proteins, and are useful for labeling secreted proteins with disulfide bridges such as antibodies...

...pAAV-DIO-BACE-HA Syn-driven, Cre-dependent Beta-Secretase to be expressed (1:1) with either ATLAS protein...

...antibodies to zebrafish cell surface receptors and secreted proteins. Return to top Other Resources Addgene...

...page for the corresponding catalog item (see maps section for images). Figure 2: AAV Pro cells were transduced...

...), including plasmids for protein production, secretion, and codon-optimized versions designed...

...phosphosite DNA library can also be introduced into a second expression vector encoding a split mCherry fluorescent...

...control of gene expression in eukaryotes. To avoid secondary effects from repurposing endogenous systems that...

...Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression pCS2+8CeGFP - Zebrafish/Xenopus/Worm...

...facilitate multiplex labeling with subclass-specific secondary antibodies. The plasmids encoding these R-mAbs..., HEK293, COS, CHO), from which the R-mAbs are secreted into the culture media. For high level expression...

...KD PINK1 GST tac Parkinson's Mark Cookson 13917 mSecPrP-pCB6+ PRNP CMV Dementia Susan Lindquist 14121 pcDNA3...pB-TR-hSOD1 SOD1 Flag CMV-TetO ALS Lukas Trantirek 232481 pHLsec hSOD1 SOD1 Flag CAG ALS Lukas Trantirek 232864 ...

...use a highly specific secondary antibody, rather than a broadly  reactive secondary (Fig. 1). For example... a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding and... a distinctly conjugated secondary antibody. Consider too that some secondary antibodies can cross react... IgG2a cross-adsorbed secondary and a Goat anti-rat IgG1cross-adsorbed secondary would limit species cross-reactivity...Benefits and Pitfalls of Secondary Antibodies: Why Choosing the Right Secondary Is of Primary Importance...applications. The desired specificity of your secondary antibody depends on whether you are using poly...poly- or monoclonal primary antibodies. Some secondary antibodies are broad and recognize multiple isotypes...

...by mixing your primary and secondary antibodies, blocking excess secondary with serum from the same species...apply your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your ...the same host species as your secondary antibody since 1) your secondary should not recognize Fab fragments...your primary and secondary antibodies before adding them to your sample so the secondary antibodies aren...antibodies - one against your target of interest and a second against the first antibody. When endogenous IgGs...species as your primary antibody are present, your secondary antibody recognizes both the endogenous IgGs and...staining with no problem! But to be sure that your secondary is not binding endogenous IgGs, you should include...

...each primary is omitted but all secondaries included to uncover secondary antibody cross-reactivity. ...antigen-binding site.      Secondary antibody specificity  In indirect staining approaches, secondary antibody specificity...and the use of isotype-specific secondary antibodies.  Baseline secondary signaling levels In addition to... substituting for a more specific secondary antibody. The secondary antibody control should be included...staining with multiple primary and secondary antibodies. Ideally, secondary antibodies will be highly specific...signal from your anti-Mouse IgG2a secondary antibody, then that secondary antibody is reacting non-specifically...experimental group, with the new kinase inhibitor and a second group, the positive control group, with an alternative...

... is what conjugated secondary antibodies you have available. If the only secondary antibodies you have... in your sample, then a fluorescently labeled secondary antibody binds that first antibody, then you use...approaches is to use sequential cycles of primary and secondary antibody labeling and imaging, repeating for each...mixture of primary antibodies and appropriate secondary antibodies. The general process is similar to ...imaged at a time, inactivating the fluorescent secondary antibodies before labeling the next target. B)...antibodies are combined in one step and all the secondary antibodies are combined in one step. As with...frozen or formalin-fixed, paraffin-embedded tissue sections, methanol- or paraformaldehyde-fixed cell samples...

...primary/labeled secondary Less sensitive than HRP-RANbody staining Requires secondary antibody staining...primary/labeled secondary   Less sensitive than HRP-RANbody staining Requires secondary antibody staining... RANbodies are expressed in 293T cells and are secreted from cells due to their IgGκ signal peptide sequence...three reporters stain cultured cells and tissue sections, some reporters are better suited for particular...summary. HRP HRP tagged RANbodies don’t require a secondary antibody for detection. Instead they are detected... signal over staining with a primary/ labeled secondary antibody and a ≥100-fold increase in signal over...directly labeled primary or a primary/labeled secondary antibody are used to detect additional antigens...

...no need for a secondary antibody incubation step, saving time. Moreover, since secondary antibodies can...primary antibody and a conjugated secondary antibody. Incorporating a secondary antibody increases the sensitivity...antigen and then a reporter enzyme conjugated secondary antibody binds to the primary antibody. In a sandwich...that binds to the antigen in a sample and then a second detection antibody binds. The detection antibody...conjugated with a reporter enzyme or a conjugated secondary antibody can be used. In a competitive ELISA a...also lack the signal amplification afforded by secondary antibodies and thus are not suitable for low abundance...sensitivity of the assay, since multiple secondary antibodies will bind to a single primary antibody and ...

...common is that secondary antibodies are generally polyclonal, which means that multiple secondary molecules...and incubation time. Secondary Antibody Unsurprisingly, this is the second antibody you apply, which...indirect IF requires two antibodies: a primary and secondary. For indirect IF, the primary antibody targets...attached to a fluorescent molecule, instead a secondary antibody that targets the primary is conjugated...it is more efficient to generate a handful of secondary antibodies that target common IgGs and then conjugate.... It is common for labs to keep a few trusted secondary antibodies on hand to mix and match with a larger...Speaking of different colors, having a palette of secondary antibodies in a few different species and colors...

...anti-rat IgG1 secondary to detect Protein A and green fluorophore-tagged anti-mouse IgG2a secondary to detect...IgG1 secondary antibody to bind to the anti-Protein A antibody and an anti-mouse IgG2a secondary antibody...constraining using the indirect method since a secondary antibody reactive to a certain isotype will bind...antibodies that are the same isotype then the secondary antibody will bind to both and you will not be...the same isotype and will react with the same secondary. If you were to change one of the antibody’s isotypes...isotypes, however, you could then use 2 different secondaries for your antibody pair and visualize both targets...multiplexed indirect staining methods since the secondary antibody used will react to both primary antibodies...