Centrifugation
Type
Protocol
...Centrifuge Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the...
We narrowed to 765 results for: Son
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Lab Safety for Biosafety Levels One and Two
TypeProtocol...Right after entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole... -
Lab Safety for Biosafety Levels One and Two
TypeProtocol...Right after entering the lab, put on the proper personal protective equipment (PPE) and wear it the whole... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...Star Activity" and can happen for a variety of reasons, including high glycerol concentration. Learn more... -
Molecular Biology Protocol - Restriction Digest of Plasmid DNA
TypeProtocol...Star Activity" and can happen for a variety of reasons, including high glycerol concentration. Learn more... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol... should be enough that they can grow out in a reasonable amount of time, but not so many that they vastly... -
Virus Protocol - Generating Stable Cell Lines
TypeProtocol... should be enough that they can grow out in a reasonable amount of time, but not so many that they vastly... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...Sodium azide is toxic. When handling, use proper personal protective equipment including laboratory coat... -
Western Blot
TypeProtocol...most proteins but more stringent buffers and a sonication step may be required for hard to extract proteins... -
Affinity Purification of Recombinant Antibodies with Protein A or Protein G
TypeProtocol...Sodium azide is toxic. When handling, use proper personal protective equipment including laboratory coat... -
Western Blot
TypeProtocol...most proteins but more stringent buffers and a sonication step may be required for hard to extract proteins... -
Neurodegeneration Plasmid Collection
TypeCollection...Myc CMV Parkinson's Mark Cookson 13314 pCMVTNT PINK1 C-myc PINK1 Myc CMV Parkinson's Mark Cookson 13315 ...CMV Parkinson's Mark Cookson 13316 pcDNA-DEST47 PINK1 C-GFP PINK1 GFP CMV Parkinson's Mark Cookson 13319...CMV Parkinson's Mark Cookson 13320 pLenti6-DEST PINK1-V5 WT PINK1 V5 CMV Parkinson's Mark Cookson 13321...GST tac Parkinson's Mark Cookson 13322 pGEX5X.1 PINK1 KD PINK1 GST tac Parkinson's Mark Cookson 13917 mSecPrP-pCB6...His, Myc CMV Parkinson's Ted Dawson 17612 pRK5-Myc-Parkin PRKN Myc CMV Parkinson's Ted Dawson 17613 pRK5...GFP CMV Parkinson's Mark Cookson 25045 pDEST53-LRRK2-G2019S LRRK2 GFP CMV Parkinson's Mark Cookson 25046...GFP CMV Parkinson's Mark Cookson 25048 pDEST53-LRRK2-Y1699C LRRK2 GFP CMV Parkinson's Mark Cookson 25049... -
Penn Vector Core Partnership with Addgene
TypeCollection... James M. Wilson AV-1-PV0105 105532-AAV1 pAAV.CMV.ffLuciferase.SV40 Control James M. Wilson AV-1-PV1917...James M. Wilson AV-1-PV1963 105542-AAV1 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M. Wilson AV-1-PV2177...James M. Wilson AV-1-PV2642 105552-AAV1 pENN.AAV.hSyn.TurboRFP.WPRE.RBG Control James M. Wilson AV-1-PV2975...Control James M. Wilson AV-2-PV0101 105530-AAV2 pAAV.CMV.PI.EGFP.WPRE.bGH Control James M. Wilson AV-2-PV1963...Control James M. Wilson AV-5-PV0102 105531-AAV5 pAAV.CMV.LacZ.bGH Control James M. Wilson AV-5-PV1917 105541...James M. Wilson AV-5-PV1963 105542-AAV5 pENN.AAV.CB7.CI.eGFP.WPRE.rBG Control James M. Wilson AV-5-PV2177... James M. Wilson AV-5-PV2213 105547-AAV5 pENN.AAV.EF1a.eGFP.WPRE.rBG Control James M. Wilson AV-5-PV2369... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...when preparing and handling lentiviral particles. Personal protective clothing should be worn at all times... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...when preparing and handling lentiviral particles. Personal protective clothing should be worn at all times... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...mCherry Maria Harrison 61206 pCMU-PDESr Plasmodesmata (Plants) PDLP1 mCherry Maria Harrison 61200 pCMU-PLAr...* Michael Davidson 54491 mCherry-Lifeact-7 Actin filaments LifeAct mCherry* Michael Davidson 54610 mEGFP-Lifeact...Michael Davidson 55148 mCherry-Tubulin-C-18 Microtubules alpha-Tubulin mCherry* Michael Davidson 12298 ...mCherry Maria Harrison *Fusions to other FPs can be found by searching the complete Michael Davidson Fluorescent...Michael Davidson 55165 mCherry-ZO1-C-14 Tight junctions Zonula Occludens-1 mCherry* Michael Davidson 55001...TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagBFP James Johnson 13050 DsRed-Rab5... 38770 pEF.myc.ER-E2-Crimson Endoplasmic reticulum ER retention signal E2-Crimson Benjamin Glick 36204... -
Brain Armamentarium
TypeCollection...Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson 221463-PHPeB pAAV_BiPVe4_eGFP AAV construct expressing...pAAV_WDL0003_dTomato_nlsdTomato Gordon Fishell James M. Wilson 218792-PHPeB pAAV_BiLAMP5e3_dTomato_nlsdTomato ...Alias: pAAV_WDS0004_GCaMP6f Gordon Fishell James M. Wilson 213947-PHPeB pAAV_BiSSTe4_GCaMP6f AAV construct...interneuron-targeting enhancer Gordon Fishell James M. Wilson 213945-PHPeB pAAV_BiSSTe4_ChR2_mCherry AAV construct...interneuron-targeting enhancer Gordon Fishell James M. Wilson 213944-PHPeB pAAV_BiSSTe4_dTomato_nlsdTomato AAV...Alias: pAAV_WDP0003_GCaMP6f Gordon Fishell James M. Wilson 213943-PHPeB pAAV_BiPVe3_GCaMP6f AAV construct ...cell-targeting enhancer Gordon Fishell James M. Wilson 213941-PHPeB pAAV_BiPVe3_ChR2_mCherry AAV construct... -
Optogenetics AAV Preps
TypeCollection... Karl Deisseroth 122063 pAAV-EF1α1.1-ChrimsonR-GFP EF1a ChrimsonR GFP Constitutive 2 Edward Boyden 124650... 5, rg* Edward Boyden 59171 pAAV-Syn-ChrimsonR-tdT Syn ChrimsonR tdTomato Constitutive 1, 5, 9, rg* Edward...Edward Boyden 62723 pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato] Syn ChrimsonR tdTomato Cre dependent 1, 5 Edward...Edward Boyden 130909 AAV-CAG-FLPX-rc [ChrimsonR-tdTomato] CAG ChrimsonR tdTomato Flp dependent 8 Edward Boyden...dependent 9 Karl Deisseroth 92207 TRE-ChrimsonR-mCherry TRE ChrimsonR mCherry Constitutive 1 Alice Ting 105448... 105448 pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Syn ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Hillel ...Adesnik 124603 pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 EF1a ChrimsonR (soma-targeted) mRuby2 Cre ... -
CRISPR Guide
TypeCollection... A., Chan, M. M., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024). Improving prime editing...including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon systems...I., Yoon, Y., Song, C., Cao, Y., Gallant, J., Xue, W., Rivera-Pérez, J. A., & Sontheimer, E. J. (2019)...S., Cofsky, J. C., Kranzusch, P. J., Sontheimer, E. J., Davidson, A. R., Maxwell, K. L., & Doudna, J. ..., J., Edraki, A., Shah, M., Sontheimer, E. J., Maxwell, K. L., & Davidson, A. R. (2016). Naturally occurring... Chen, C., Nelson, J. W., Newby, G. A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu,...Ramadoss, G. N., Shi, Q., Hung, K. L., Samelson, A. J., Pogson, A. N., Kim, J. Y., Chung, A., Leonetti... -
Control AAV Preps
TypeCollection...PHP.eB James M. Wilson 105531 pAAV.CMV.LacZ.bGH CMV LacZ Constitutive 5, 8 James M. Wilson 105532 pAAV.CMV.ffLuciferase.SV40...Constitutive 8 James M. Wilson 105534 pAAV.TBG.PI.LacZ.bGH TBG LacZ Constitutive 8 James M. Wilson 105536 pAAV.TBG.PI.Null.bGH... 8 James M. Wilson 105539 pAAV.hSyn.eGFP.WPRE.bGH Syn EGFP Constitutive 1 James M. Wilson 200773 AAV-cfGFP...5 James M. Wilson 105535 pAAV.TBG.PI.eGFP.WPRE.bGH TBG EGFP Constitutive 8 James M. Wilson 105542 pENN.AAV.CB7...James M. Wilson 105543 pENN.AAV.cTNT.PI.eGFP.WPRE.rBG cTNT EGFP Constitutive 9 James M. Wilson 105544 pENN.AAV.CB7...James M. Wilson 105548 pENN.AAV.CMVs.TurboRFP.WPRE.RBG CMV TurboRFP Constitutive 1, 8 James M. Wilson 105549...James M. Wilson 105552 pENN.AAV.hSyn.TurboRFP.WPRE.RBG hSyn TurboRFP Constitutive 1 James M. Wilson 105554...