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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 770 results for: VARS

Showing: 41 - 60 of 770 results

  1. CRISPR Plasmids and Resources

    Type
    Collection
    ...Addgene's CRISPR collections feature plasmids for a variety of organisms, including mammals, plants, bacteria...an empty vector for your experiment based on a variety of factors. gRNAs gRNA plasmids to target specific...

  2. Retrovirus Plasmids

    Type
    Collection
    ...immortalized cells (see Weinberg Lab page for resistance variants) Weinberg 1773 pBABE-hygro-hTERT MoMLV Expression...expression; also expresses GFP. Also see pMIG-w , a variant that has WRE, a post-transcriptional regulatory...

  3. Antibody Production

    Type
    Collection
    ...applications. The specific QC experiments performed varies for each antibody catalog item. Whenever possible...primers targeting the heavy chain and/or light chain variable regions. The PCR product is purified, sequenced...

  4. mTOR Pathway

    Type
    Collection
    ...mTORC2 The better-characterized mTORC1 responds to various signals, including amino acids, stress, oxygen,...prognosis in many cancers, including breast, colon, ovarian, and liver cancers. mTORC1 is activated downstream...

  5. Promega Plasmid Collection

    Type
    Collection
    ...which have been developed for special functions that vary from purification, visualization, and functional...specific purpose. HaloTag technology is optimized for various protein expression systems including E. coli , ...

  6. AAV Packaged on Request

    Type
    Collection
    ...standards and typical titers, though actual yields may vary by construct. Size Quantity Total Volume Minimum...processing your order. MTA Approval 1+ business days, varies by requesting organization Viral vector preps remain...

  7. p53 Pathway

    Type
    Collection
    ...: 24739573 . Uncovering the role of p53 splice variants in human malignancy: a clinical perspective. Surget...Germline TP53 mutations and Li-Fraumeni syndrome. Varley JM. Hum Mutat. 2003 Mar;21(3):313-20. PubMed PMID...

  8. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...express your gene of interest under the control of a variety of promoters. Professor Oka and the Gene Vector...can be used to generate adenovirus expressing a variety of reporters as well as the Cre and Flp recombinases...

  10. COVID-19 Resources

    Type
    Collection
    ...Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants Return to top Other Coronavirus Plasmids: SARS-CoV...Screening Jesse Bloom Tyler Starr Libraries of variant Spike protein RBD mutations for comprehensive mapping...

  12. Cre-Lox and Other Site-Specific Recombinases

    Type
    Collection
    ...Site Canonical Sequence Structure (Length) Common Variants Cre loxP ATAACTTCGTATAgcatacatTATACGAAGTTAT 13...results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination events but...

  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...create cell lines with endogenously-tagged gene variants or browse Addgene’s entire collection of Stem ...c-Myc from polycistronic cassettes KLF4 N-terminal variance modulates induced reprogramming to pluripotency...

  14. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...effective brightness of a fluorescent protein may vary depending on experimental conditions. pKa is the... to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue to...

  15. Western Blot

    Type
    Protocol
    ...science. General Guidelines Acrylamide percentage varies in SDS-PAGE gels. Use lower percentage acrylamide...buffer. Pro-Tips The volume of lysis buffer will vary depending on the size of your cell pellet but will...between 250–1000 µL . The ideal lysis buffer will vary depending on the cellular location of the protein...sample. Pro-Tip The ideal total protein loaded will vary between samples and target proteins but is typically... 1X TBST. Pro-Tip The ideal blocking buffer will vary between antibodies. Refer to the manufacturer's ...blocking buffer. Pro-Tip The optimal concentration will vary between antibodies but is typically between 1–10...

  16. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...increases reproducibility, as it eliminates the variation associated with repeated transient transfection...lots can promote or inhibit transfection. Test a variety of brands and FBS lot numbers to find one suitable...transduction efficiency. Infection efficiency will vary between cell lines. It is not recommended that lentiviral...that already contain 0.5 mL of virus solutions at various dilution. Make sure to use the polybrene-containing...transgene has integrated in different locations in the various cells in the culture. This is because lentiviral...of the lentivirus used, cells may also exhibit varying numbers of integration events. In other words, ... transgene in their genome. This can result in varying expression levels of the transgene from different...

  17. Immunocytochemistry

    Type
    Protocol
    ...will vary depending on the sample type and the protein of interest. You may need to try a variety of fixation... Pro-Tip The optimal antibody concentration will vary but generally ranges from 1-10 µg/mL. Add 500 µL... target. The optimal antibody concentration will vary between antibodies. Review the manufacturer's instructions...

  20. Zinc Finger Consortium Reagents

    Type
    Collection
    ...Joung and Daniel Voytas have deposited at Addgene various reagents for engineering and expressing zinc finger...
Showing: 41 - 60 of 770 results