We narrowed to 651 results for: ESP

1. Addgene's eBook Collection

   Type

   Guide

   ...CRISPR 101 en español January 2024 | First Edition, trans. About CRISPR 101 en español January 2024... First Edition, trans. About Una traducción al español de nuestro libro electrónico, CRISPR 101. Close...

2. CRISPR 101: Off-Target Effects

   Type

   Blog Post

   ...correlate with catalytic activity at those sites, especially when DNA mismatches are involved. Two other approaches...

3. Viral Vectors 101: AAV Variables That Matter

   Type

   Blog Post

   ..., Y., & Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator...

4. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

   Type

   Blog Post

   ...Commons 4.0 license. The research groups responsible for Easi-CRISPR have also recently developed ...

5. CRISPR 101: Epigenetics and Editing the Epigenome

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   Blog Post

   ...transcriptional activation more than dCas9-VP64, especially when targeting distal enhancers. As the effects...

6. PiggyBac-ing Through the Genome Editing Field

   Type

   Blog Post

   ...for integration at regions of open chromatin, especially at promoter or exonic regions. If the cell’s ...

7. Finding Your Perfect Job After University

   Type

   Blog Post

   ... very strong beliefs in traditional medicine, especially when I knew that there was a cheap and easy treatment...

8. When Fidelity Matters: A frank discussion about ligase fidelity

   Type

   Blog Post

   ...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ...

9. RNA Interference in Plant Biology: New Tools for an Old Favorite

   Type

   Blog Post

   ... precursors of rice and Arabidopsis species, respectively. Short hairpin RNA (shRNA) is conceptually similar...

10. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    ... problem. It is estimated that mycoplasma is responsible for up to 60% of the cell culture contamination...

11. A History of Genome Engineering in Popular Culture

    Type

    Blog Post

    ...present-day frogs via genetic engineering. The corresponding movie came out in 1993 and sequels of Jurassic...

12. Deep Dive: Statistical Tests (Comparisons)

    Type

    Blog Post

    ...family-wise error and the false discovery rate, respectively. Many other tests exist to identify significant...

13. Plasmids 101: Using Transposons in the Lab

    Type

    Blog Post

    ... likely than viral vectors to induce an immune response and are also easier and cheaper to generate. Gene...

14. Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering

    Type

    Blog Post

    ...expression of the int and xis genes which are responsible for excision of mini-λ from the host’s chromosome...

15. Plan Your Experiment

    Type

    Guide

    ...manipulations used in mammalian cells and their corresponding Cas enzymes. In the next section, we will cover...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...necessary to optimize your delivery conditions, especially if you are using a difficult cell type. For more...

16. Plan Your Experiment

    Type

    Guide

    ...manipulations used in mammalian cells and their corresponding Cas enzymes. In the next section, we will cover...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...necessary to optimize your delivery conditions, especially if you are using a difficult cell type. For more...

17. Plasmids 101: Modular Cloning Applications and Kits

    Type

    Blog Post

    ...attention to protein modifications which can be especially difficult in alternative expression systems such...

18. Live and Let Dye: Self-Labeling Protein Tags

    Type

    Blog Post

    ... HaloTag reacts faster for some dyes, others, especially some nonfluorescent ligands, react more efficiently...

19. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type

    Blog Post

    ...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably...

20. How to Deposit Your Plasmids with Addgene

    Type

    Blog Post

    ...workflow.) Our spreadsheet contains columns that correspond to the plasmid data we need; you can copy and...