We narrowed to 637 results for: ESP
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TypeBlog Post... likely than viral vectors to induce an immune response and are also easier and cheaper to generate. Gene...
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When Fidelity Matters: A frank discussion about ligase fidelity
TypeBlog Post...fidelity") better than T4 DNA Ligase (21, 25, 26). Despite this higher fidelity, Taq DNA Ligase can still ... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...expression of the int and xis genes which are responsible for excision of mini-λ from the host’s chromosome... -
Deep Dive: Statistical Tests (Comparisons)
TypeBlog Post...family-wise error and the false discovery rate, respectively. Many other tests exist to identify significant... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post... HaloTag reacts faster for some dyes, others, especially some nonfluorescent ligands, react more efficiently... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog Post...attention to protein modifications which can be especially difficult in alternative expression systems such... -
A Primer on Optogenetics: Introduction and Opsin Delivery
TypeBlog Post...any neuroscience project or topic of interest. Despite its power and versatility, the technique is remarkably... -
Plan Your Experiment
TypeGuide...manipulations used in mammalian cells and their corresponding Cas enzymes. In the next section, we will cover...transgene expression with AAVs can be long-lived, despite recombinant AAVs not integrating into the host ...necessary to optimize your delivery conditions, especially if you are using a difficult cell type. For more... -
How to Deposit Your Plasmids with Addgene
TypeBlog Post...workflow.) Our spreadsheet contains columns that correspond to the plasmid data we need; you can copy and... -
CRISPR 101: Cytosine Transversion Editors
TypeBlog Post...strand that does not base pair with the guide RNA. Despite being called the "non-target strand" in CRISPR ... -
CRISPR 101: Cytosine and Adenine Base Editors
TypeBlog Post...NHEJ), HDR occurs at a relatively low frequency, especially in nondividing cells. Scientists everywhere wistfully... -
Using AAV for Neuronal Tracing
TypeBlog Post...single synaptic step, thus preventing further widespread expansion of RABV. This improvement resulted ... -
15 Hot Plasmids from 2017
TypeBlog Post...function or cause cellular growth delays. With eSpCas9(1.1) and coselection, Agudelo et al. achieved editing... -
CRISPR Guide
TypeGuide...Overview Bacteria have an interesting adaptive immune response that detects and eliminates foreign DNA, which...fidelity enzymes, and their mechanisms, include: eSpCas9(1.1) - weaken interactions between the HNH/RuvC...sequence The sequence within the gRNA that is responsible for Cas9 binding, it does not include the 20 ... -
Gamma-Retroviral Vector Guide
TypeGuide...entry. Biosafety should always be considered with respect to the precise nature of experiments being performed...of a molecule or substance to induce an immune response in the body. in cis In the context of viral vector... -
Molecular Biology Reference
TypeGuide..., and guanine (abbreviated to A, T, C, and G respectively) that are organized into a double stranded helix... selective pressure to retain the plasmid DNA, despite the added replication burden, as bacteria without... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...for spatial proteomic mapping in living cells. Despite these advances, the Ting lab’s first-generation...