We narrowed to 671 results for: FIE

...Optical Switches Plan Your Experiment References The field of optogenetics integrates optics and genetic engineering..., and the neuron is activated. In the past, the field of optogenetics was considered to include both actuators...have each since grown and developed into their own fields. In this overview, we will focus on the common ...ions into or out of the cell. Scientists have identified a variety of naturally occurring microbial opsins...variants that inhibit neurons have been created and identified in other species. By acting as light-gated chloride...

...the library information to see if it should be amplified before you conduct your screen. If you need to...negatively affect data reproducibility. Figure 1: Simplified flow chart for amplifying and using a pooled ... conduct a pooled library screen. Figure 2: A simplified outline for using lentiviral gRNA libraries in...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their cDNA...

...putative knockout allele must be experimentally verified Cut Nick Homology Directed Repair (HDR) Generate..., you can also directly deliver mature mRNA or purified protein into cells. These methods are more transient... This method is a common choice for generating modified mouse lines. Protein delivery Lastly, you can ...mRNA to protein. Instead, you directly combine purified Cas protein and in vitro transcribed gRNA to form...

...proteins. These are antibody mimics instead of modified antibodies and are not closely related to antibodies... with: a positive control, a sample previously verified to contain your protein of interest (in ELISAs...released from the complex and can be sequenced and amplified for analysis. Overview of application Process ...assays. This method is suitable for DNA:histone modifier interactions, which have strong binding between...

.... To do this, your DNA fragment must first be amplified with specific Gateway attB1 and attB2 sites attached...endonucleases. These endonucleases cut dsDNA at a specified distance away from the recognition sequence. Cutting...cloning, but instead of an in vitro reaction with purified enzymes, it takes advantage of the powerful recombination...

...different types of DREADDs that can be broadly classified by the signaling protein that the receptors couple...inhibit neuronal signaling. PSAM-based LGICs can be classified by the ion pore domain they are coupled to: PSAM-Gly...chemogenetic control in a single molecule driven by FRET-modified bioluminescence. Neurophotonics 11 (2), 021005...

...2018). Differential viral accessibility (DIVA) identifies alterations in chromatin architecture through... can infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic...

... adaptive immune system in bacteria, have been modified for genome engineering. Find essential background...

... transcribed by the RNA polymerase. Figure 1: Simplified promoter region during transcription. Created...

...Complementation Toolkit Plant Expression Benjamin Field 50 plasmids for assembling bimolecular fluorescence...

... can infect and replicate in. Viral vector The modified form of a wild-type virus used to deliver genetic...