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In Category

We narrowed to 768 results for: Dos

Showing: 721 - 740 of 768 results

  1. Typing CRISPR Systems

    Type
    Blog Post
    ...adaptive immune functions that other CRISPR types do, due to a lack of canonical CRISPR features (Pinilla-Redondo...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... a new or experienced reprogrammer? Which methods do you have experience using? Let us know about your...multiple transfection makes it difficult to control the dose of plasmid the cells receive over the whole reprogramming...

  4. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ... significant medical interest, however efforts to do so have been hindered by a lack of tools, compounds...target soluble kinase domains, but these domains often do not express well in E. coli without their partner...cells. The mutant cells were treated with WNV at a dose and duration sufficient to kill all control cells...and not specific loci. To overcome this flaw, the Zoldos lab has developed CRISPR tools for epigenome editing...and their effects on gene regulation. Professor Zoldos and colleagues from the Department of the Biology...deletion efficiency was dependent upon 1) Cas9 and gRNA dose (both of which can be modulated using the different...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...surface. Tare the weighing boat or paper. You need to do this because you don’t want to include the weight...

  6. AAV ddPCR Titration

    Type
    Protocol
    ...entire dilution plate with Microseal adhesive seal - do not press the film, just gently cover so nothing ...collecting drops along the side of the tube. Cap gently - do not push the cap in all the way, just ensure the ...

  8. Water Bath Protocol

    Type
    Protocol
    ...of the tube. This way, as items are floating, you do not need to necessarily maneuver the bottles or tubes...

  10. Video Library

    Type
    Protocol
    ...Protocol Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...Agudelo et al. prevent ouabain binding to ATP1A1 but do not compromise the ion pump’s function or cause cellular...

  12. Centrifugation

    Type
    Protocol
    ... centrifuge and be dangerous for the user. If you do not have a balanced number of samples, you may need...

  14. Immunocytochemistry

    Type
    Protocol
    ... and a negative control sample such as cells that do not express the protein of interest....titrating your antibody to determine the optimal dose. To ensure that your antibody is both functioning...

  15. General Transfection

    Type
    Protocol
    ...8x10 5 cells in a T75 flask in a volume of 15 mL. Do not add antibiotics to the media. Use cells that ...

  16. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...designed by Eric Greenwald and the Jin Zhang Lab at UCSD Do you have suggestions for other plasmids that should...Proc Natl Acad Sci U S A. 2008 Jan 8. Wolfgang Dostmann cGMP (cyclic GMP) Green fluorescent probe for ...

  17. Lentivirus Production

    Type
    Protocol
    ...10 5 cells in a T75 flask in 15 mL DMEM Complete. Do not add pen-strep to the media. Use cells that are...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...class II, DO alpha HLA-DNA, HLA-DZA, HLADZ HLA-DOB major histocompatibility complex, class II, DO beta DOB...180015. doi: 10.1038/sdata.2018.15. PMID 29485622 Do you have suggestions for other plasmids that should...
Showing: 721 - 740 of 768 results