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Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Learn what non-model organisms are, why scientists use them, and what they can teach us.

Get the basics on RNA interference (RNAi), how it compares with CRISPR, and tools for RNAi studies in plants.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.

Learn how to transform E. coli with your plasmid of interest.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

Get tips on choosing the best fluorescence microscopy technique for your next imaging experiment.

Explore empty plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest.

Learn about selecting the correct pipettor and pipette tip, how to dispense liquids, and how to handle the pipette.

Learn about new tools in Addgene's collection, including new prime editing and CRISPR applications, tools for neuroscience research, and more!

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

How to purify plasmid DNA from a bacterial culture, including protocols, tips, and FAQ

Lab protocol for transfection of HEK293 cells to produce recombinant antibody.

Tips on designing primers for PCR

Fourteen years after Cas9, CRISPR has moved beyond DNA cutting. Read Addgene's recap of CRISPR Frontiers 2026, covering programmable gene insertion, RNA-sensing cell killing, and next-gen base and prime editors.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

Protocol for creating a glycerol stock, including additional tips for long-term storage.