We narrowed to 1,027 results for: ELL

... Anti-Pan-Neurofascin (extracellular) [A12/18R] Pan-Neurofascin (extracellular) Rat Mouse IgG2a 177439...Anti-Kv1.4 K+ channel (extracellular) [L71/5R] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 177489...Mouse IgG2a 177564 Anti-ADAM22 (extracellular) [N57/2R] ADAM22 (extracellular) Mouse Mouse IgG2a 177565 Anti-GluN2B...Anti-Kv1.4 K+ channel (extracellular) [L71/22] Kv1.4 K+ channel (extracellular) Rat Mouse IgG2a 190292...-Neurofascin (extracellular) scFv [A12/18] A12/18 scFv 2t Pan-Neurofascin (extracellular) Rat Mouse 190486...Kv1.4 K+ channel (extracellular) scFv [L71/5] L71/5 scFv Kv1.4 K+ channel (extracellular) Rat Mouse 190518... Mouse 190537 LRP4 (extracellular) scFv [N207/27] N207/27 scFv LRP4 (extracellular) Mouse Mouse 190538...

...integrase to insert the viral genome into a host cell. Scientists can take advantage of this to stably...integrate transgenes of interest into a variety of cell types. Wild-type gamma-retroviruses have been engineered...of Large T antigen for creation of immortalized cells. See the Weinberg Lab page for additional resistance...Expression of hTERT for creation of immortalized cells. See plasmid 1771 for puromycin selection, and plasmid...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified version...MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV For...tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

... pAAV-mDlx-GFP-Fishell-1 Dlx GFP Constitutive 1, 2, 5, 8, 9, rg*, PHP.eB Gordon Fishell 99130 pAAV-mDlx-NLS-mRuby2... pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre dependent 1, 2, 8, 9, rg* Gordon Fishell 83894 pAAV-hDlx-Flex-dTomato-Fishell...pAAV-hDlx-Flex-dTomato-Fishell_7 Dlx dTomato Cre dependent 1, 2, 5, 9, rg* Gordon Fishell 100043 pAAV.synP.DIO.EGFP.WPRE.hGH...pAAV-CBh-mKate2-IRES-MCS (WT-WT) CBh mKate2 Constitutive 2 Marcella Patrick 114469 pAAV-CaMKIIa-mCherry CaMKIIa mCherry...

...nucleotide variant lines in an isogenic cellular background. All cell lines and phenotypic datasets that result...CRISPR Guide CRISPR Protein Tagging Plasmids for Stem Cell Research Neurodegeneration Research Collection The...Plasmids The following plasmids can be used to create cell lines with endogenously-tagged gene variants or ...or to differentiate human iPSCs into other cell types, as noted in the purpose field: ID Plasmid Description...neurodegenerative diseases with genomic engineering: A new stem cell initiative from the NIH. Neuron . 2021 Apr 7;109...

...-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific genomic...compatible with fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting... multiple genomic loci, and compatible with live cell imaging. Compared to techniques like fluorescence... for detecting the chromatin dynamics in living cells. The fluorescent CRISPR system has been used for...repetitive loci can be efficiently visualized in living cells using a single gRNA that has multiple targeted sequences...

...kinetic properties as well as improve the expression and membrane targeting in host cells. Other microbial ...switch tools to activate, inhibit, and manipulate cellular processes. Plasmid...to blue light and allows cations to flow into the cell. Variants of channelrhodopsin have been engineered...actively move cations or anions into or out of the cell. When using these opsins as a tool to control neuronal...plasmids that use light to control a protein or cellular activity. For example, search "Cdc42", "transcription...

...interest and correspond to edited and non-edited cell populations. For more information, see the Hsiau...nucleases (e.g. base editing). Developed by the Luca Pinello Lab (Link opens in a new window) . For more details...), clone (primer design), and evaluate gRNAs, as well as predict off-targets, for CRISPR in 180+ genomes...window) A sgRNA design tool for genome editing, as well as gene regulation (repression and activation). ...identify novel gRNA target sites in a single gene, as well as a target site common to a set of similar sequences...138 KB Musunuru CRISPRs in human pluripotent stem cells pCas9_GFP ; gRNA empty vector Protocol at StemBook...Bauer Protocol for Genomic Deletions in Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder...

...expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between...application-specific validation and quality control by Addgene as well as by our trusted partner labs. Learn More Cloning... research purposes include animals or hybridoma cells, but are difficult to generate due to expense and...monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from an individual...transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted into the culture media ...

...maintenance. Worms are particularly well suited for studies in cell biology, development, and aging. Scientists.... elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging and fluorescence...microscopy experiments. The C. elegans genome is well studied and hundreds of reporters have been developed...researchers to express desired constructs in precise cell types and/or developmental timeframes. Here we highlight...detection of genome-editing events. Developed in Craig Mello's lab and described in A Co-CRISPR Strategy for Efficient...

...reporters for cell cycle analysis. Puromycin selection. Kevin Brindle and Duncan Jodrell 17448 pLenti ...Lentivirus can transduce both dividing and non-dividing cells without a significant immune response. These viruses... plasmid 46948 for additional variant. Stephen Elledge 44011 pInducer10-mir-RUP-PheS 3rd Lentiviral shRNA...et al. (2011) for additional variants. Stephen Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression...expression of hTERT, used to create immortalized cell lines. See Hayer et al. (2016) for additional selection...

...function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008 , Foley & Yeh et al., PLoS ONE... Townsend et al., Nature 2009 , Zou et al., Cell Stem Cell 2009 ). Resources A detailed protocol for practicing...ZFNs that function in zebrafish, plants, and human cells Kits...

...unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Neurexins and Neuroligins), and common epitope tags, as well as antibody plasmids to create or express plasmid-based...mission, IPI is providing access to high-quality, well-characterized antibodies in an open science model...highly conserved proteoglycans crucial for regulating cell signaling during development. The IPI collection...antibodies that target epitope tags and other common cellular proteins. ID Recombinant Antibody Description ...

...mammalian expression vectors, human embryonic kidney cells are transfected with the plasmid(s) encoding the.... This buffer is not compatible for use in live cells and will interfere with conjugation. For applications...antigen is first transiently expressed in mammalian cells. To learn which specific QC experiments were performed...email [email protected] . Immunocytochemistry (ICC) Cells are fixed and labeled with the antibody and a species-specific...Western Blotting (WB) Proteins are extracted from cells, separated by SDS-PAGE, transferred to a membrane...

...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 hM4D(Gi) - Inhibition NLS-dTomato none 1, 9, rg* Gordon Fishell 83897 pAAV-hDlx-GqDREADD-dTomato-Fishell...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 hM3D(Gq) - Activation NLS-dTomato none 1, 9, rg* Gordon Fishell 50472 pAAV-GFAP-HA-rM3D... chemically induce neuronal activity in specific cell types. See our Chemogenetics Plasmids and Chemogenetics...

... Ralstonia solanacearum Rickettsia sp. Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp...Species Anabaena sp. Bacillus sp. Burkholderia sp. Bordetella pertussis Campylobacter sp. Chlamydia sp. Clostridium...reading frames for vaccinia virus - Bennink and Yewdell Labs Yarrowia lipolytica Golden Gate tool kit :...applications like recombinant protein purification and cell-free circuit design in E. coli - Freemont Lab MoClo-YTK...

...abundant forms of posttranslational modification in cells and research into its many roles in protein function...interactions can be discovered by fluorescence-activated cell sorting (FACS). The Mode #2 phosphosite library ... Barber, K. W., Ter Haar, C. M., Rogulina, S., Amrofell, M. B., Isaacs, F. J., Rinehart, J., & Jewett,... production of recombinant phosphoproteins using cell-free protein synthesis . Nature Communications ,...TBK1 activation to promote mitophagy . Molecular Cell , 60 (1), 7–20. https://doi.org/10.1016/j.molcel...

...YPet-pBAD - Bacterial Expression ZsYellow1 529 539 8 Tetramer ZsYellow1-N1 - Mammalian Expression mPapaya1...allowing tracking of processes such as intracellular trafficking or cell cycle dynamics. Protein Excitation...Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared Long Stokes Shift...Cloning Vector - Mammalian Expression Return to top Yellow Protein Excitation (nm) Emission (nm) Brightness...

...be used for screening transcription factors for cellular programming or cancer drug resistance....Zhang Lab Plasmids The Human TFome Library Stem Cells Protocols & References MORF Plasmids MORF Pooled...transcription factors for applications such as cellular programming or cancer drug resistance. Protocols...Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F. Cell 2023 Jan 5; 186(1):209-229. PubMed (Link opens in...

...important to normal cell signaling and physiology, and, when dysregulated, can enable cancer cell survival. Although...that is essential for regulating the growth of the cell. p53 p53, the most frequently mutated gene in human...somatic variants found in lung adenocarcinoma as well as variants found across cancer types as part of...

...then be used to infect permissive cells that express ACE2, the cellular receptor for SARS-CoV-2, in a single...firefly luciferase to simultaneously measure the # of cells containing reactivated latent provirus and the overall...strength of viral transcriptional response in these cells. Warner Greene 105621 Lenti-luciferase-P2A-Neo Lentiviral...

...George Church Cynthia Collins James Collins Tom Ellis Drew Endy Tobias Erb Susan Golden John Gray Jeff... Keasling Taek Soon Lee Joshua Leonard Han Lim Wendell Lim Timothy Lu Michel Maharbiz Thorsten Mascher...Sensing and Signaling Browse plasmids related to intercellular signaling and environmental sensing. Strains... Circuit Transfer C. Collins Lab Quorum Sensing Ellis Lab GeneGuard Endy Lab Logic Gates , BIOFAB Promoter...

...or genetic elements of interest to introduce in cells. A packaging plasmid (or Rep/Cap plasmid) containing...or herpes simplex virus, to replicate in the host cell and complete the lytic cycle. To avoid the use of...provided by helper plasmids and specific packaging cell lines. Helper plasmids generally encode the adenoviral... General Transfection AAV Production in HEK293T Cells AAV Purification by Iodixanol Gradient Ultracentrifugation...

...and cloning backbones for red, yellow, and turquoise FRET pairs Gadella et al. (2023) mScarlet3: a brilliant...influenced by many factors, such as the organism or cell type, relative position and orientation of the fluorophores... (Unpublished) FRET Indicators - Live and Fixed Cells Additional Resources at Addgene Learn more on the...

...-mDlx-ChR2-mCherry-Fishell-3 Dlx ChR2 mCherry Constitutive 1, 9, rg* Gordon Fishell 135634 pAAV-S5E2-ChR2...Optogenetics AAV Optogenetic tools can be used to control cell activity in living tissue with light. Optogenetics...Fluorophore GFP Red-wavelength fluorescent protein Yellow-wavelength fluorescent protein Blue-wavelength ...

... Modeling Neuroendocrine Tumors Using Adult Stem Cell-Derived Organoids Matthew Meyerson Dana-Farber Cancer...Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou Harvard University...University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley Bernstein Broad Institute...

...Collection Empty Backbones Blue Cyan Green mEmerald Yellow Orange Red Photoactivatable The lab of Michael ...plasmids are available to industry scientists as well as academic and nonprofit labs. Want to learn more...mEmerald Plasmid Description Industry Return to top Yellow Plasmid Description Industry Return to top Orange...

...Institute for Cell Science Plasmid Collection E11 Bio PRISM Collection New England BioLabs Cell-Imaging Plasmid...Botman-Teusink Yeast FP Collection Protein Expression Cell Migration Consortium Malate Dehydrogenase CUREs ...

...to activate programmed cell death or dormancy for phage-infected bacterial cells so as to limit the spread...population. Type VI enzymes that function in mammalian cells can be used to attentuate RNA levels. In mammalian...

...Cas protein and a target-specific gRNA in the same cell at the same time. All-in-one plasmids ensure that...both the Cas and gRNA are delivered to the same cells, but are limited to the function of the Cas protein...an additional plasmid containing a Cas enzyme or cells or organisms in which Cas has been integrated into...

...Tesson et al., Nat Biotechnol. 2011 ) Human somatic cells ( Miller et al., Nat Biotechnol. 2011 ; Reyon and...., Nat Biotechnol 2012 ) Human pluripotent stem cells ( Hockemeyer et al., Nat Biotechnol. 2011 ) TALE...TALE transcriptional activators in human cells ( Maeder et al., Nat Methods 2013 ) More detailed information...