...#103005) . Note on PHP.eB tropism and specific mouse lines: Enhanced CNS tropism exhibited by the PHP.eB serotype...element Calcium sensor GCaMP Dimidschstein 100854 pAAV.Syn.NES-jRGECO1a.WPRE.SV40 Syn Calcium sensor RGECO ...

...are used to knockout, activate or repress target genes. Number of gRNAs - A given CRISPR library may target...target the entire genome or a specific class of genes (subpools). Species - the gRNAs within a given CRISPR...

...Harvard Medical School Klaus Hahn , UNC Richard Hynes , MIT Rick Horwitz , University of Virginia Denise...

...Browse plasmids expressing genes relevant to Parkinson's disease created by the Michael J. Fox Foundation...

...plasmids for transcriptional repression of target genes....

...expression of recombinant phosphoproteins - Rinehart FreeGenes Project Open Enzyme Collection Screening Multiplexed...

...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 51306 pUC57-Simple-gRNA backbone T7...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759 pT7-gRNA T7 BsmBI In vitro transcription...

...Stockholm Bacterial Expression Plasmids (empty backbones/controls): ID Plasmid GenBank Key Features 26092...

...developed class of tools are Luminopsins (LMOs), or luminescent opsins: fusion proteins of a light-emitting luciferase...

...constitutive overexpression of FPs and tagging of genes of interest with FPs in yeast. Characterization ...

...format include a variety of popular plasmids and backbones with high cloning potential. By making these plasmids...

...biosafety of viral vectors, and general biosafety guidelines. Addgene Blog: Viral Vector Posts Content from...

...project has received funding from the Innovative Medicines Initiative 2 Joint Undertaking under grant agreement...

...factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids by Model Organism Bacteria CRISPR...

...editing systems include targeted AID-mediated mutagenesis (TAM) from the Chang lab and CRISPR-X from the...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 47549 pDD162 (Peft-3::Cas9 + Empty...

...extracellular protein interactions. Sun Y, Gallagher-Jones M, Barker C, Wright GJ. Analytical Biochem. 2012...

.... Since this starter cell now has all the viral genes present, the virus can spread from this cell to ...

...then be transfected into mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral...

...may ship at room temperature. However, Addgene determines ideal shipping conditions for each order based...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49410 pCFD3-dU6:3gRNA dU6:3 BbsI Injection...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49014 pRPR1_gRNA_ handle_RPR1t pRPR1...

... account. We will notify you by email within 1 business day regarding your account verification. Once ...

...2019 Aug 28;5(8):eaaw1822. Scheeren and Verdoes Backbones for mouse recombinant antibodies A panel of recombinant...

...catabolic processes including autophagy and lysosome biogenesis. mTORC2 does not respond to nutrients, but it...

...Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae...

... plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences...

...combination with Cre-dependent transgenic mouse lines or co-infection with Cre dependent viral vectors...

...Wong BK, Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, Goldowitz D, Wasserman WW, Simpson ...

...However, polybrene is toxic to some cell lines. In these cell lines, substitute protamine sulfate for polybrene...the RNAi consortium for targeting human and mouse genes. Protocols... directed against 15,000 human and 15,000 mouse genes. Addgene is working with the TRC to make this shRNA...that can be accessed after a free registration. Guidelines for designing siRNAs with effective gene silencing...polymerase III. Note that these were the prevailing guidelines when the pLKO.1 vector was created. Additional... least 3 nucleotide mismatches to all unrelated genes. TIP: Addgene recommends that you select multiple...Vector The following steps were the prevailing guidelines when the pLKO.1 vector was created. For alternative...

...container Fire blanket Fire extinguisher Guidelines BSL-1 Guidelines Before You Work Right after entering ...chemical hygiene officer. BSL-2 Guidelines Remember, the BSL-1 laboratory guidelines above are expected to be...These lab safety guidelines provide steps to ensure you are working in BSL-1 and BSL-2 labs safely. Protocols...be followed in addition to BSL-2 guidelines below, including PPE protocols . Working in a BSL-2 laboratory...

... but can be adapted to a variety of target cell lines and selection markers. Note this assay requires ...Millipore TR-1003-G 1X PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...transduction efficiency. Titer will vary between cell lines. It is not recommended that lentiviral supernatants... to multiple freeze-thaw cycles. This protocol outlines the seeding of the cells at the same time as the... the virus-mediated transduction. For some cell lines, transduction is optimized if the cells are seeded...

...to support reproducibility in science. General Guidelines Acrylamide percentage varies in SDS-PAGE gels...Nonfat milk powder 96-well microtiter plate Chemiluminescence substrate Plastic wrap Primary antibody Secondary... at RT on a shaking platform. Prepare the chemiluminescence substrate by mixing 1:1 reagent A to reagent...reagent B. Gently incubate the membrane in the chemiluminescence reagent for 5 min at RT . Cover the membrane...with clear plastic and use a gel imager with chemiluminescence detection or a dark room to detect the bands...

...hundreds of µL, the second is tens, and the third is ones. Therefore 100 µL would read as 100 from top to ...P20: The first digit is tens of µL, the second is ones, and the third (red) digit is tenths. Therefore,...2.2 µL would read as 022. P2: The first digit is ones of µL, the second red digit is tenths, and the third...at the bottom. For the P1000, this represents the ones. For the P200, this represents the first decimal...

...Then streak horizontal lines in another section of the plate, and then diagonal lines in another section ...very popular technique is to draw in discontinuous lines. Start by streaking a vertical line of bacteria ...

...DNA in other lanes contaminating your sample. To accomplish this, it is best to skip lanes between samples...couple simple ways to increase the resolution (crispness) of your DNA bands include: a) running the gel...

...restriction enzyme -based cloning to test individual clones before use of more expensive forms of plasmid verification... want to know how to differentiate between two clones that differ only in the orientation of the insert...Simply run the digest products on a gel and those clones with the appropriately sized products should have...

...Addgene's Business Development Manager, discusses his transition from the Scientist Team to Business Team,...CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR ...