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We narrowed to 207 results for: pcr

Showing: 151 - 200 of 207 results

  1. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

    Type
    Blog Post
    ...and these rare events are easily detectable using PCR. The system also functions in S. pneumoniae and can...created two “pCRISPomyces” systems for use in Streptomyces (Cobb et al., 2015). pCRISPomyces-1 includes ...array, while pCRISPomyces-2 contains Cas9 and a gRNA cassette. The simpler system of pCRISPomyces-2 displays...pCas9: carries Cas9 and chloramphenicol resistance pCRISPR: carries a spacer targeting the gene of interest...machinery are first electroporated with pCas9. Then, pCRISPR is introduced along with an oligonucleotide repair...advantages including shorter plasmid curing time. pCRISPomyces for editing in Streptomyces  Streptomyces bacteria...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...annealed synthetic oligos - eliminating the need for PCR or additional molecular cloning steps during plasmid...occurred at higher frequency and could be detected by PCR while large deletions were relatively rare. The vectors...target G-protein coupled receptors (GPCRs) yet one third of the GPCRs in the human genome are orphan receptors...Mammalian Expression 74334 pCR8-Magneto2.0-p2A-mCherry Gateway 74333 pCR8-Magneto2.0 Gateway 74307...are unknown. Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts...allows researchers to interrogate the druggable human GPCR-ome using a quick, affordable, and ubiquitous reporter...recruitment assay incorporating 314 codon-optimized GPCR sequences for human cell line expression. In a twist...

  5. Video Library

    Type
    Protocol
    ...commonly used technique for molecular cloning, such as PCR- or restriction enzyme-based cloning. Purifying DNA...maintaining sterility while working. AAV Titration by qPCR Use qPCR to measure the physical titer of your adeno-...adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology Protocol Agarose Gel...

  9. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...minutes at 95°C in a PCR machine or in a beaker of boiling water. If using a PCR machine, incubate the...Days post-infection mRNA knockdown (quantitative PCR) ≥3 days Protein knockdown (western blot) ≥4 days...

  11. Guide to Using Pooled Libraries

    Type
    Guide
    ...surviving cells are collected, their plasmids are PCR-amplified and sequenced using NGS to identify their...

  12. Molecular Biology Reference

    Type
    Guide
    ...single-stranded DNA sequence used as an initiation point for PCR amplification or DNA sequencing of the plasmid. Primers...

  13. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ...controlling GPCR activation have been developed to selectively study individual types of GPCRs with greater...be used for lower frequency stimulation. GPCR signaling GPCR signaling is a complicated process involving...change the membrane potential. OptoXRs are modified GPCRs. When exposed to light, they activate downstream... coupled receptors  G-protein coupled receptors (GPCR) regulate cellular function through multiple intra-cellular...have been developed for utilizing light to control GPCR activation and downstream signaling cascades (reviewed...Levitz, 2020), including light-sensitive chimeric GPCRS (OptoXRs) (Tichy et al., 2019), photoswitchable ...rhodopsin, and the intracellular domain of an endogenous GPCR, such as adrenergic receptors. When exposed to light...

  14. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ...requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have ddPCR experience...with ddPCR, but do have experience with qPCR, you could talk about how your experience with qPCR would...that by describing how you developed and used a ddPCR assay to answer an experimental question. Now, the...manager knows that you can design experiments with ddPCR, interpret the results, and develop a successful...would make it easy to master ddPCR.  Finally, it’s a good idea to state why you’re applying to the specific...

  15. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...biosensors kit By Mike Lacy G protein-coupled receptors (GPCRs) are critical components of many physiological pathways...targets. To enable large-scale profiling of many GPCRs, Mikel Garcia-Marcos’ lab recently developed a collection... detection of activation triggered by endogenous GPCR. Example applications include large-scale pharmacological...The authors used this platform to assay dozens of GPCRs across many cell types in varying conditions, revealing...2024). Direct interrogation of context-dependent GPCR activity with a universal biosensor platform. Cell...

  16. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...titer results between ddPCR and qPCR? A: At CLOVER, we have traditionally used qPCR (protocol link). We ...this to strongly bias titer. We are also adopting ddPCR, which does not a standard curve and somewhat more...

  17. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...titration by qPCR using SYBR green technology Here’s some advice on how to successfully set up your qPCR to determine...generate a standard curve, visit the AAV Titration by qPCR written protocol page. Getting started with tissue...

  18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ...signaling pathways G protein-coupled receptors (GPCRs) detect molecules outside of the cell and translate...those signals into cellular responses. For example, GPCRs and their downstream G protein partners can regulate...have a long standing interest in the biology of GPCRs and G proteins, with an emphasis on oligomerization...

  19. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...clinical and diagnostic labs, deep sequencing and qPCR methods are too costly and time intensive to process...platform on clinical stool samples by comparison to qPCR and demonstrated that it can also detect expression...mRNA expression cannot be identified with DNA-based qPCR methods, the ability to detect mRNA transcripts ...

  21. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...designer drugs, are G protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to... threshold and silence neurons. DREADD-activated GPCR signaling can also modify other properties such ...

  22. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ... loop regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022)...produce more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides...

  23. Trimmer Lab NeuroMab Collection

    Type
    Collection
    ...promoters (orange). Hybridoma-derived V L and V H domain PCR products are fused to a joining fragment comprising...translocation of the heavy chain across the ER membrane. PCR-mediated fusion of these three elements is followed...

  24. Cre-lox system

    Type
    Collection
    ...pBS500 EF1alpha-GFPcre Cre-GFP fusion EF-1 alpha Mammalian Sauer 11923 pBS598 EF1alpha-EGFPcre Cre-EGFP fusion...EF1alpha-EGFPcre* Cre-EGFP fusion EF-1 alpha Mammalian Sauer 11956 pBS594 promoterless EGFPcre Cre-EGFP...tet-hCMV-EGFPcre Cre-EGFP fusion Tet inducible Mammalian Sauer 11958 pBS532 promoterless GFPcre promoterless...-hCMV-GFPcre tet inducible Cre-GFP fusion tet-hCMV Mammalian Sauer 11961 pBS596 tet-hCMV-GFPcre tet inducible...Cre EF-1 alpha Mammalian Sauer 11919 pBS448 RSV-GFPcre Cre-GFP fusion Rous sarcoma virus (RSV) LTR/promoter...Tamoxifen-inducible Cre None Zebrafish Stankunas 82696 pCRE-iRFP670 Cre and iRFP670 PGK Mammalian Mullen 84032...

  25. Immunology Research Plasmids and Resources

    Type
    Collection
    ...PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2 PSMB8 proteasome...receptor 2 CYSLT2, CYSLT2R, GPCR, HG57, HPN321, KPG_011, PSEC0146, hGPCR21 DARC Duffy blood group, chemokine...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR SAA1... estrogen receptor 1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPR30, LERGU, LERGU2, LyGPR, MGC99678 GPHA2 ...repeat-containing G protein-coupled receptor 6 FLJ14471, GPCR, VTS20631 LHB luteinizing hormone beta polypeptide...relaxin/insulin-like family peptide receptor 3 GPCR135, MGC141998, MGC142000, RLN3R1, RXFPR3, SALPR RXRA...

  26. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...quality control processes as our in-stock service: ddPCR, purity testing, and sterility testing, plus viral...

  27. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...new optogenetic tool called PdCO.  This is an "optoGPCR" (coupled to Go, so it's an inhibitory opsin!)...

  32. Brain Initiative Collection

    Type
    Collection
    .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV8 .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 139505-AAV9 .... Used for photoswitchable control of inhibitory GPCR signaling cascades. Michael Bruchas 140135-AAV1 ...

  38. Hot Plasmids - August 2020

    Type
    Blog Post
    ... of the new viral preps from recent months: New GPCR activation-based norepinephrine (GRABNE) sensors...

  43. Luciferase Plasmid Collection

    Type
    Collection
    ...based on a split NanoLuc® have been used to assess GPCR-G protein interactions , protein dimerization , ... firefly luciferase and GFP James Wilson 106457 pCR3.1-Luc Firefly CMV Mammalian expression of firefly...

  44. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Parkinson's Mark Cookson 29354 pCR8-DJ1-WT PARK7 Parkinson's Mark Cookson 29355 pCR8-DJ1-D149A PARK7 Parkinson's...Parkinson's Mark Cookson 29356 pCR8-DJ1-E64D PARK7 Parkinson's Mark Cookson 29357 pCR8-DJ1-M26I PARK7 Parkinson's...-LFA EIF2AK2 GAL VWM disease Harmit Malik 20123 PCRII-zSynC SNCB Lewy body dementia Aaron Gitler 20648...SOD1 GFP CMV ALS Elizabeth Fisher 26573 CaV2.1 (pCR3.1) CACNA1A CMV Spinocerebellar ataxia 8 Diane Lipscombe...

  46. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...eabq8173. Yulong Li Norepinephrine nLight red and green GPCR-based NE indicators Sensitive multicolor indicators...Tommaso Patriarchi Norepinephrine GRAB_NE family of GPCR activation-based NE sensors A Genetically Encoded...

  47. CRISPR Guide

    Type
    Collection
    ... is necessary for Cas enzymes to bind target DNA PCR P olymerase C hain R eaction; Used to amplify a specific...

  49. Microbiology Resources

    Type
    Collection
    ...three-part fusion proteins - Planas and Santalucia Labs pCri System : Heterologous protein expression in E. coli...

  50. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...lab Cas9 species = S. pyogenes (PAM = NGG) 42875 pCRISPR BsaI E. coli, S. pneumoniae Kanamycin none, need...
Showing: 151 - 200 of 207 results