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Showing: 61 - 80 of 925 results

  1. Zinc Finger Consortium: Zinc Finger Arrays

    Type
    Collection
    ...finger nucleases (ZFNs) are effective tools for making targeted mutations in zebrafish. With support ...OZ526 OPEN OPEN tCCCATCCTAtcgttGCATGAGCTc mdka (midkine-related growth factor) OZ527 and OZ528 OPEN OPEN...engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML et al....

  2. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Gene Vector Core (Link opens in a new window) have kindly deposited a series of lentiviral and adenoviral... College of Medicine Gene Vector Core have also kindly provided this protocol detailing the process for...gene of interest from the PGK (phosphoglycerate kinase I) promoter pCDH-CMV4 72284 Express gene of interest...

  3. Genetic Code Expansion

    Type
    Collection
    ...Genetic Code Expansion You may also like... GCE4All: Making Genetic Code Expansion Accessible Using Phosphoserine...with multiple copies of the tRNA. Applications By making small changes in selected amino acids within a ...there are several things to consider. If you are working off of a previously established protocol, make ...PylT_FLAG-AbKRS-chIPYE chimeric PylRS Chimeric photo-crosslinking-lysine Mammalian TAG Simon Elsaesser 140021 ...

  4. Cre-lox system

    Type
    Collection
    ...interest by removing neoR and stop sequence; used for making transgenic mice. Plasmid 15037 also contains IRES-EGFP...interest, removes Neo and stop cassette; Contains flanking arms for Rosa26 integration; See similar plasmid...transfection efficiency, selecting desired clones, and making transgenic mice. ID Plasmid/Article Description...fluorescent proteins, resulting in the indelible marking of mouse neurons with multiple distinct colors ...

  5. Luciferase Plasmid Collection

    Type
    Collection
    ...Renilla luciferase have short protein half-lives, making them useful as transcriptional reporters. However...Gaussia luciferase is secreted and more stable, making time course experiments possible, but its short... flank the MCS for improved normalization. John Atkins 167576 mpCHECK2 Firefly/Renilla Insertion of 3'...

  6. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...dPspCas13b does not require a Protospacer Flanking Sequence (PFS), making it a very flexible editing system. ...

  7. Ginkgo Bioworks COVID-19 Collection

    Type
    Collection
    ...viral proteins as a first step for many different kinds of important experiments. In an effort to speed ...Codon-optimized expression (recode) Truncated for making pseudovirus (Ctrunc) ID Plasmid Description Mutations...

  8. Plasmid Collections

    Type
    Collection
    ... page to help you find what you're looking for. If you are looking for a non-plasmid item, browse our ...

  9. Chemogenetics Plasmids

    Type
    Collection
    ...luciferase and a light-sensing optogenetic element, making a bimodal opto-chemogenetic actuator. When the ..."KORD AAV", "LMO", "Sternson", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  10. Optogenetics Plasmid Collection

    Type
    Collection
    ...engineered or identified to increase the spectral and kinetic properties as well as improve the expression and...Cre inhibition", "Boyden 2014", or other terms. Looking for viral preps? We also offer ready-to-use AAV...

  11. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand DNA...target DNA using a gRNA and recruits Cas3. After nicking the non-targeted strand, Cas3 starts unraveling...

  12. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...plasmids and backbones with high cloning potential. By making these plasmids available as cgDNA, we hope to aid...assistance at [email protected] . Don’t See What You’re Looking For? At this time, Addgene has made a limited number...

  13. Resolute Plasmid Collection

    Type
    Collection
    ...Initiative 2 Joint Undertaking under grant agreement No 777372. This Joint Undertaking receives support ...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...Depositing Labs Hal Alper J. Chris Anderson Adam Arkin Gabor Balazsi Matthew Bennett Carolyn Bertozzi Nicolas...biological systems for novel functions. Addgene is working with leading synthetic biologists around the world...

  16. CRISPR Plasmids - Drosophila

    Type
    Collection
    ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  17. CRISPR Plasmids - Yeast

    Type
    Collection
    ...dsDNA repair templates with homology to the DNA flanking the DSB and a specific edit close to the gRNA ...error-prone non-homologous end joining (NHEJ). Double nicking strategies reduce unwanted off-target effects. ...

  18. AAVED

    Type
    Collection
    ...Guerin, and Leila Haery from Addgene will also be speaking or leading workshops. Tentative Meeting Agenda...resources for this meeting's publication, we are asking participants to share practical information in ...

  19. Microbiology Resources

    Type
    Collection
    ...accordance with export laws and biosafety regulations. Looking for E. coli or yeast plasmids? Addgene has a wide...Engineering blog post to see how Addgene depositors are making this possible in a range of organisms. Find CRISPR...

  20. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ... Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin...protocol videos available that may be helpful for working with this plasmid collection. Single cell passaging...
Showing: 61 - 80 of 925 results