...Addgene’s comprehensive high-quality quality control process includes titering, purity assessment, sterility...

...plasmids to your cart, then complete the checkout process online. Click here for additional ordering information...

...opens in a new window) and an optimized sorting process. This combination allows IPI to generate antibodies...

...artificially activate or inhibit specific biological processes. Addgene’s collection contains two main classes...

...measure and manipulate cells and their biomolecular processes....

... second guide within a U6-guide cassette. This process is compatible with all pX458 family plasmids. Additional...

...the user through the REAL or REAL-Fast assembly process or lists of plasmid ID numbers required for FLASH...

... Service Information Production Our production process and quality control, with examples of quality control...

...SynBio Deposit Guide or start our online deposit process . Browse SynBio Plasmids by Category Cloning and...

...antibody production methods and quality control processes for purified recombinant antibodies....

...screen for genes involved in specific biological processes. For more information, see our Guide to Plasmid...

...Förster resonance energy transfer, or FRET , is a process by which energy is non-radiatively transferred ...

...1, which may require additional lab biosafety procedures. Additionally, because this virus integrates ...

...condensed multiplexing capabilities , as it can process multiple gRNAs under a single promoter, and can...which include Cas13a , Cas13b , and Cas13d , can process crRNA precursors to mature crRNAs. Upon ssRNA recognition...

...conventional HDR-mediated gene knock-in. The detailed procedure can be found in Sakuma et al., Nature Protocols...

... one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production. Bhadra S, et al. bioRxiv...

... tags can be used to simplify the purification process. Using plasmids in this collection, you can highly...

...neurons with vesicular stomatitis virus vectors. Proc Natl Acad Sci USA 108, 15414–15419. PubMed PMID:...Further, the connections between regions are often reciprocal, forming a neuronal loop which is activated and...

...output from the first process to the second process. For example, our bbduk process created trimmed FASTQ...pipeline, we wrote a separate Nextflow process. In Nextflow, a process defines which command or script will...specifying the output of an initial process as the input to a subsequent process, we linked each step in our ...pipeline steps into processes and then connect the steps using the output from one process as input to the... Nextflow process.  Next, we specified the input and output channels for the initial process and tested...was clear: to build one process at a time and connect each subsequent process to the existing ones. Once... our first process was working as expected, we moved forward with creating the next process and linking...

...material transfer agreement (MTA) process. Even though this process is essential to sharing plasmids around...requests and signatures via an online process, though the process has changed a lot over the last twenty...can make our process easier for others.  If you’ve still got questions about our MTA process, you can check...every time they wanted to share materials, and the process was time-consuming and lengthy. So the NIH called...called a meeting in 1990 to discuss the process, which eventually led to the creation of a standardized ...ensure that we can facilitate a fast and agile MTA process. This is important to keep up with the number of...averaged facilitating 89,477 MTAs per year.  The MTA Process at Addgene As a repository, Addgene doesn’t actually...