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We narrowed to 100 results for: dcas9, grna

Showing: 61 - 80 of 100 results

  1. CRISPR Plasmids for Genomic Visualization

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Preps Protocols gRNA Design Tools CRISPR Blog Posts Using catalytically inactive Cas9 (dCas9) fused to a ...multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs with target...fluorescent marker like GFP, researchers have turned dCas9 into a customizable DNA labeler compatible with ...fluorescence microscopy in living cells. Alternatively, gRNAs can be fused to protein-interacting RNA aptamers...it is easy to implement due to the simplicity of gRNA design, programmable for different genomic loci,...efficiently visualized in living cells using a single gRNA that has multiple targeted sequences in close proximity...

  2. Plan Your Experiment

    Type
    Collection
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit. Science... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ... proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...your gRNA is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ...Regulation Activate dCas9 fused to an activator peptide activates gene expression. Interfere dCas9 alone, or fused...methods can be used to visualize genomic loci. gRNAs Empty gRNA Vectors Select an empty vector for your experiment...experiment based on a variety of factors. gRNAs gRNA plasmids to target specific genes. Browse Plasmids... : Browse depositor protocols, find software for gRNA design and deep sequencing analysis, discover links...repressor peptide, inhibits transcription. Epigenetics dCas9 fused to an epigenetic modifier alters chromatin...powerful tool for genome-wide screening. Purify dCas9 fused to an epitope tag can be used to isolate a...

  4. CRISPR Guide

    Type
    Guide
    ... of unique gRNAs. gRNA design for CRISPR libraries is usually optimized to select for gRNAs with high ...achieved by using orthogonal dCas9s (e.g. S. pyogenes dCas9 and S. aureus dCas9) tagged with different fluorescent... specified by a particular gRNA. Epitope tag(s) are fused to dCas9 or gRNA for efficient purification ...CRISPRa CRISPR Activation; using a dCas9 or dCas9-activator with a gRNA to increase transcription of a target...CRISPRi CRISPR Interference; using a dCas9 or dCas9-repressor with a gRNA to repress/decrease transcription... guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short synthetic...commonly referred to as an sgRNA. gRNA scaffold sequence The sequence within the gRNA that is responsible for...

  5. Plan Your Experiment

    Type
    Guide
    ...genome sequence dCas9 or dCas9 fusion with transcriptional repressor (such as dCas9-KRAB) dCas9-KRAB can be...edit type, designing and cloning a gRNA, delivering Cas and the gRNA, and validating the edit. Science... system for Cas9 and the guide RNA (gRNA), the design of the gRNA sequence, and finally how to validate...modifying the genome sequence dCas9 fusion with transcriptional activator (such as dCas9-VP64) Many different ... proper gRNA design. While there are some cases where it makes sense to manually design a gRNA, in most...most cases gRNA design software is better and more efficient at designing potential gRNAs for you to choose...your gRNA is the balance between on-target and off-target activity. In a perfect world, your gRNA sequence...

  6. Lentiviral Prep Service

    Type
    Collection
    ...Resistance Activity PI 61422 dCAS9-VP64_GFP dCAS9 (D10A, H840A) none Expresses dCAS9-VP64 activator with 2A ...RNA guide (gRNA) that harbors targeting sequences. Browse our repository for a list of gRNA plasmids targeting...dCAS-VP64_Blast dCAS9 (D10A, N863A) Blasticidin 3rd generation lenti vector encoding dCAS9-VP64 with 2A ...containing 56,762 unique sgRNAs. This backbone does not contain dCas9. 56,762 unique sgRNAs targeting 18,885 ... Doench , Root 73179 Human gRNA pooled library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2...57,050 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,050 unique...57,011 unique sgRNAs along with 500 non-targeting controls. This backbone does not contain dCas9. 57,011 unique...

  7. CRISPR Plasmids - Epigenetics

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...have fused catalytically dead dCas9 to epigenetic modifiers. Design your gRNA to target a specific promoter...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts To make targeted ...

  8. CRISPR References and Information

    Type
    Collection
    ...at Addgene Church gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 ...DNA FLAG tagged dCas9 PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF,...activation dCas9 activators sgRNA dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator...Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; Cas9 ; optimized ...optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo...

  9. CRISPR Plasmids - Parasites

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Eukaryotic parasites...forward genetics of these parasites. Moreover, CRISPR-dCas9 gene regulation has also been established for some...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the... - Toxoplasma gondii CRISPR genome-wide knockout gRNA pooled library designed with 10 guides against 8,158...

  10. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...genome. gRNA Empty Vectors Many gRNA empty vectors have been deposited at Addgene. To find the gRNA vector... Cas9, dCas9, or dCas9-VP64 along with 1-4 sgRNAs expressed from independent promoters. sgRNAs are cloned...Empty gRNA expression vectors for insertion of custom gRNA target sequences. CRISPR...target-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and a Cas ...pyogenes Puro Zhang phH1-gRNA 53186 Mammalian BbsI none S. pyogenes Gersbach pmU6-gRNA 53187 Mammalian BbsI...pyogenes Gersbach phU6-gRNA 53188 Mammalian BbsI none S. pyogenes Gersbach ph7SK-gRNA 53189 Mammalian BbsI...

  11. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Two classes of base... nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...editors are targeted to a specific locus by a gRNA, and they can convert cytidine to uridine within a small...

  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids...Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The table below lists gRNA plasmids designed to... questions to consider when choosing a gRNA: Does a given gRNA sequence exactly match your genomic target...Variation between a given gRNA sequence and your genomic target may reduce the gRNA activity. The variety ...pyogenes , S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible...gRNA expression vectors available from Addgene. CRISPR...

  13. CRISPR Plasmids - Xenopus

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Insert Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...) at a specific location based on a co-expressed gRNA-defined target sequence. DSBs are preferentially...surrounding the DSB is introduced along with the Cas9 and gRNA plasmids, the cell may instead repair the break ...single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can...

  14. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Type Promoter PI Publication Empty gRNA Expression Vectors Select a gRNA expression plasmid based on factors...-specific gRNA in the same cell at the same time. Single plasmids containing both the gRNA and Cas protein...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The following CRISPR...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and a specific edit close to the gRNA PAM site. When a repair template is present, the...single category (cut, nick, etc.) On the other hand, gRNA plasmids that do not co-express a Cas protein can...

  15. CRISPR History and Development for Genome Engineering

    Type
    Collection
    ...with paired gRNAs to lower off-target cleavage frequency. Truncated gRNAs: Truncated gRNAs display less...epigenetic marks. Visualize : Fluorescently labeled dCas9 or gRNAs that bind fluorescent proteins can be used ...Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow. Nat Biotechnol . ...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR revolution... 2: An overview of CRISPR and NHEJ/HDR. The Cas9/gRNA complex produces a DSB at the target genomic locus...is not only simple, but also scalable. Multiple gRNAs targeting multiple loci can be easily expressed ...it especially amenable to genetic screens. Pooled gRNA libraries can be used to identify genes that are...

  16. CRISPR Plasmids - Single-Strand Break (Nick)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands of your DNA in close...

  17. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ... protein dCas9 is expressed along a CRISPR array that contains 20 bp "addresses", where dCas9 is going...You may also like... CRISPR Guide CRISPR Protocols gRNA Design Tools CRISPR Blog Posts The David Bikard ...going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression of the RNA polymerase...for quick integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed... Bikard, D., & Van Teeffelen, S. (2018). Tuning dCas9’s ability to block transcription enables robust,...Schematic of the strain expressing two reporters and dCas9 under a P-tet promoter integrated in the chromosome...

  18. CRISPR Plasmids - RNA Editing

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Type VI CRISPR systems...

  19. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Fully functional ...double-strand break (DSB) at a specific location based on a gRNA-defined target sequence. DSBs are preferentially...flanking the DSB and 2. a specific edit close to the gRNA PAM site. When a repair template is present, the...

  20. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian...CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3 is a unique ...off-target alterations and require only a single gRNA. Cas3 must be paired with the Cascade ( C RISPR-...Cas11. Cascade is directed to target DNA using a gRNA and recruits Cas3. After nicking the non-targeted...
Showing: 61 - 80 of 100 results