Skip to main content
Addgene
Showing: 61 - 80 of 625 results

  1. TALEN Engineering

    Type
    Collection
    ...desired length: the REAL (Restriction Enzyme And Ligation) assembly method (Sander et al., Nat Biotechnol...al., manuscript submitted), and the FLASH (Fast Ligation-based Automatable Solid-phase High-throughput)...

  2. p53 Pathway

    Type
    Collection
    ...acetylation, which disrupt its interaction with negative regulators, increase its stability and DNA binding...activate transcription, and they also have a dominant negative effect on functional p53 through oligomerization...

  3. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...used with Clover pGWF1 Cyan & Yellow Bacterial Gateway-compatible vector to express a gene of interest...pmTurquoise2-T2A-Venus(L68V) Brightness standard used a negative control (no FRET) with pmVenus(L68V)-mTurquoise2...

  4. Viral Vectors

    Type
    Collection
    ... While plasmids are typically used to encode, propagate, and manipulate genetic information, viruses are...research typically do not have the ability to propagate in the same way that naturally occurring viruses...

  5. Open Enzyme Collection

    Type
    Collection
    ... Open Enzyme parts have C/E overhangs for Golden Gate assembly. Plasmids Explore the collection and order...Lab Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

  6. Zhang Lab's CRISPR Frequently Asked Questions

    Type
    Collection
    ...digest should discriminate between positive and negative clones. Clones with insertion will show only linearized...Forward: CCATCCCCTTCTGTGAATGT EMX1-Reverse: GGAGATTGGAGACACGGAGA Why does the PX260 plasmid use 30nt oligos...

  7. Plan Your Experiment

    Type
    Collection
    ...your experimental goal, you are ready to start navigating the different reagents that are available for...containing the gRNA scaffold using standard restriction-ligation cloning. However, the exact cloning strategy will...

  8. Cre-lox system

    Type
    Collection
    ...pENTR/D_creERt2 Cre-ERT2 - Tamoxifen inducible; Gateway entry vector none Entry Vector Zon 27493 pBT140... pET-(-30)GFP-9xGGS-Cre-6xHis Cre fused to supernegatively charged GFP variant T7 Bacterial Liu 62657 ...

  9. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... experimental design, biosensors can enable investigation of a signaling pathway or measurement of a biomolecule... variants with different binding affinities or negative-control constructs used to characterize the sensor's...

  13. Depositor Collections

    Type
    Collection
    ...To help you find what you're looking for, we've gathered our depositor collections on one page. We hope...

  16. AAV Viral Preps

    Type
    Collection
    ... viral vector preps are purified by density centrifugation. Addgene’s comprehensive high-quality quality...

  17. All Antibodies

    Type
    Collection
    ... section of our product pages. We also include negative results when an antibody’s use is not recommended...

  18. Plasmid Collections

    Type
    Collection
    ...Addgene's repository contains 158,197 plasmids . We've gathered our curated plasmid resources, kits, and depositor...

  19. AAV Molecular Tools

    Type
    Collection
    ...Flp-dependent Flp-dependent expression of the voltage-gated Na+ channel mNaChBac and (physically separate) tdTomato...
Showing: 61 - 80 of 625 results