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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 363 results for: gne

Showing: 61 - 80 of 363 results

  1. Human Germline Editing Using CRISPR

    Type
    Blog Post
    ...donated skin, blood and semen samples. Ma et al. designed plasmids containing Cas9 and two different gRNAs... at 3 days post fertilization. Since Ma et al. designed a repair template with two synonymous mutations...thus quieting some fears about the creation of “designer babies.” The differences in editing between iPS...

  2. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    ...machine-learning model to help them construct “designer” channelrhodopsins with desired characteristics...experiments. The sequences in this library were designed using structural information about parental channelrhodopsins...existing channelrhodopsins CoChR and/or ChR2(H134R). Designer channelrhodopsins paired with a systemic AAV allow...

  3. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651 (Set D) Knockout...

  4. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    ...ability to correctly assemble a lacI/lacZ cassette (designed by NEB for use in optimization of assembly systems...methodology. To further push the test system, we redesigned for 12 and 24 fragments.  This redesign was guided...fragment versions of the lacI/lacZ cassette were designed and synthesized. In conjunction with BsaI-HFv2...

  5. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...We should note that these vectors are primarily designed for bicistronic expression; however, many could...the table above (and their related plasmids) are designed to have one or more of the genes replaced.  Additionally...save on your desktop. The Plasmids 101 eBook is designed to educate all levels of scientists and plasmid...

  6. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...biorender.com   Another method of harvesting uses magnetic beads. In this method, beads are incubated with...suspension. Antibodies bind ligands on the beads and a magnet is used to separate the beads from the media (Brechmann... Chotteau V. Antibody capture process based on magnetic beads from very high cell density suspension. ...

  7. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... Hub is here to help!  The Addgene Data Hub is designed to help scientists understand how our vectors ...images demonstrating vector performance) will be assigned citable DOIs through DataCite before being made...the Data Hub. These minimum data standards are designed specifically to allow scientists to evaluate how...

  8. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate the targeting...information on HDR and CRISPR. Our CRISPR 101 series was designed to help explain the basic principles driving CRISPRs...et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  9. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC. The ...project while he was a Postdoc in Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC.   Dylan...Dylan Kwart is a graduate student Marc Tessier-Lavigne’s lab at the Rockefeller University. He currently...

  10. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...comments section.  FX cloning kit This kit is designed to be used with Type IIS restriction enzymes and...et al. "A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo...(2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein purification...

  11. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski...Aggarwal et al., 2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants... HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these...

  12. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes. One consists of NLuc fused...Reporters. The LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection...acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET...

  13. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...domain (FokI). Zinc finger nucleases were first designed in 2005. The DNA-binding domain can be engineered...editors, new amino acid recognition sites had to be designed each time we wanted to target new genomic sites... in genome editing is prime editing, which was designed in 2019. It’s based on a prime editor which is...

  14. Validated gRNA Sequences

    Type
    Collection
    ...pyogenes, S. aureus etc.) was this gRNA sequence designed for? A given gRNA sequence may only be compatible... to indicate the Cas9 application the gRNA was designed to accomplish. Validated gRNA Sequence Datatable...

  15. CRISPR Guide

    Type
    Collection
    ...Increasing Cas9 PAM flexibility CRISPR systems designed for more precise edits require PAM sequences in...with base editing. While many base editors are designed to work in a very narrow window proximal to the...to target paralogs or lncRNAs . Guide RNAs are designed in silico and synthesized (Figure 8A), then cloned...

  16. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...Feng Zhang’s lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic ...your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer as compared...

  17. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...

  19. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ... bring this into your target organism. We have designed a Golden Gate system based on the daily needs ...produced will have different overhangs, they can be designed such that they go back together only one way and...
Showing: 61 - 80 of 363 results