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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 368 results for: gne

Showing: 61 - 80 of 368 results

  1. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ... Hub is here to help!  The Addgene Data Hub is designed to help scientists understand how our vectors ...images demonstrating vector performance) will be assigned citable DOIs through DataCite before being made...the Data Hub. These minimum data standards are designed specifically to allow scientists to evaluate how...

  2. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...CRISPR plasmids that have been designed for use in mammalian systems....Expression The following CRISPR plasmids have been designed for use in mammalian expression systems. Cut Fully...

  3. CRISPR Plasmids - Plants

    Type
    Collection
    ...CRISPR expression plasmids that have been designed for use in plants.... Posts The following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR...

  5. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...increase the frequency of edits, researchers have designed self-cleaving plasmids that liberate the targeting...information on HDR and CRISPR. Our CRISPR 101 series was designed to help explain the basic principles driving CRISPRs...et al. PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  6. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type
    Blog Post
    ...Ludwig-Maximilians-University in Munich and Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC. The ...project while he was a Postdoc in Marc Tessier-Lavigne’s lab at the Rockefeller University in NYC.   Dylan...Dylan Kwart is a graduate student Marc Tessier-Lavigne’s lab at the Rockefeller University. He currently...

  7. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...comments section.  FX cloning kit This kit is designed to be used with Type IIS restriction enzymes and...et al. "A transcription blocker isolated from a designed repeat protein combinatorial library by in vivo...(2015): 31-37. pCri system The Gomis-Ruth lab designed the pCri system to simplify the protein purification...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski...Aggarwal et al., 2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants... HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these...

  9. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes. One consists of NLuc fused...Reporters. The LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection...acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...domain (FokI). Zinc finger nucleases were first designed in 2005. The DNA-binding domain can be engineered...editors, new amino acid recognition sites had to be designed each time we wanted to target new genomic sites... in genome editing is prime editing, which was designed in 2019. It’s based on a prime editor which is...

  11. CRISPR References and Information

    Type
    Collection
    ...organisms. Developed by the Jin-Soo Kim Lab . Cas-Designer (Link opens in a new window) Searches for targets...a a low probability of off-target effects. Cas-Designer integrates information from Cas-OFFinder and Microhomology...

  12. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...ng gDNA, and H 2 O up to 20 μl. Use the primers designed in step 2 above. Conduct PCR for “non-deletion.... Optimize PCR conditions for each primer pair designed based on testing the bulk sorted cells. Run samples...

  13. CRISPR Pooled gRNA Libraries

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    Collection
    ...Doench and Root 3rd 4 78,637 Broad GPP Humagne Set C and Humagne Set D 172650 (Set C) 172651 (Set D) Knockout...

  14. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...Feng Zhang’s lab at the Broad Institute and was designed and cloned by Neville Sanjana. This fantastic ...your gRNA of interest to mammalian cells. It was designed to have increased lentiviral titer as compared...

  15. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...is absolutely fascinating to see the living art designed by creative minds with a (I assume) very steady...Our Microbiology Resource Page CRISPR plasmids designed for use in bacteria Deposit Your Microbiology ...

  17. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ... bring this into your target organism. We have designed a Golden Gate system based on the daily needs ...produced will have different overhangs, they can be designed such that they go back together only one way and...

  20. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...Organoid EasyTag workflow combines thoughtfully designed plasmid components with Fluorescence Activated... heavy optimization. Claudia Vickers’s lab has designed novel SL fluorescent biosensors by integrating...
Showing: 61 - 80 of 368 results