We narrowed to 1,082 results for: TOR;
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TypeBlog Post...acoustic reporter genes for real-time in vivo monitoring of tumors and tumor-homing bacteria. Nat Biotechnol...
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CRISPR-based Genome Editing Systems For Editing in E. coli
TypeBlog Post.... The pDonor plasmid pRE57-Ter with multiple terminators can serve to interrupt genes (or gene clusters... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post...to RNA deaminase ADAR2 to create a specific RNA editor. Cas13 enzymes are advantageous because they do... -
Career Insights: Technical Support Specialist
TypeBlog Post... and exciting to listen to the many individual stories and get (sometimes refreshing) insights into how... -
Lab to Office Culture Shock
TypeBlog Post...industry, nonprofit organization, law office, journal editor, etc.--so everyone’s experience will be a little... -
Healthcare Consulting: A Door to the Business of Life Sciences
TypeBlog Post...given therapeutic approach brings to patients, doctors, and insurance providers, and is crucial to how... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...clone the PCR products into a pBluescript cloning vector and sequence the resulting plasmid. It can take... -
Pouring LB Agar Plates
TypeProtocol...your plates are ready for use or storage at 4 ℃. You should not store your plates for longer than 1 month...material to reduce condensation. The plates are then stored at 4 ℃ until use. Once your plates have solidified... -
CRISPR 101: Validating Your Genome Edit
TypeBlog Post...performing a single base pair edit with a prime editor. What next? When generating a knock-in mutation... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...to drive cell type-specific delivery of a viral vector (Strebinger et al., 2023). All this flexibility... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...currently a research scientist in the Department of Laboratory Medicine at the University of Washington. She... -
Protocol - How to Streak a Plate
TypeProtocol...loop Bunsen burner (or other small flame source) Incubator Marker Reagents LB agar plate (with appropriate...resistance and your initials. Labeling within a laboratory setting is important for organization, and it... -
Protocol - Over-Agar Antibiotic Plating
TypeProtocol...spreading of antibiotic makes it easy for an investigator to conveniently plate and select transformed...spreading Bunsen burner (or other small flame source) Incubator Reagents 6 cm diameter LB/agar plates without ... -
Water Bath Protocol
TypeProtocol... lab! Introduction A water bath is a piece of laboratory equipment that helps bring your materials to .... Water baths can also be placed in walk-in refrigerators to achieve a temperature between 4°C and room... -
Deep Dive: qPCR
TypeBlog Post...19246619.Downey N. Interpreting melt curves: An indicator, not a diagnosis. IDT, 2014. Dwight Z; Palais ... -
Coomassie Purity Stain of Recombinant Antibodies
TypeProtocol...a blank chart to your spreadsheet. In the Chart Editor , select the Chart Type drop down menu and select...calculate this for you as follows: In the Chart Editor , select the Customize tab. Select the Series drop... -
Western Blot
TypeProtocol...microcentrifuge tube. Use the lysate immediately or store at -80 °C until ready to use. Section 2: Determine...the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the plastic tray. Place... -
DNA Quantification
TypeProtocol... to protein (260/280) is generally used as an indicator of the purity of DNA samples. These days, many... -
Protocol - How to Perform Sequence Analysis
TypeProtocol...important for your experiments. There are a number of factors to consider when selecting sequencing primers, ... -
Weighing Reagents Protocol
TypeProtocol... reagents for a stock mixture is an essential laboratory technique, as imprecise measurements can affect...